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dc.contributor.author
Carrera Silva, Eugenio Antonio  
dc.contributor.author
Silvestroni, A.  
dc.contributor.author
Leblanc, Jean Guy Joseph  
dc.contributor.author
Piard, J. C.  
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Savoy, Graciela  
dc.contributor.author
Sesma, Fernando Juan Manuel  
dc.date.available
2018-02-19T17:12:36Z  
dc.date.issued
2006-11  
dc.identifier.citation
Carrera Silva, Eugenio Antonio; Silvestroni, A.; Leblanc, Jean Guy Joseph; Piard, J. C.; Savoy, Graciela; et al.; A thermostable α-galactosidase from Lactobacillus fermentum CRL722: Genetic characterization and main properties; Springer; Current Microbiology; 53; 5; 11-2006; 374-378  
dc.identifier.issn
0343-8651  
dc.identifier.uri
http://hdl.handle.net/11336/36718  
dc.description.abstract
α-Galactosidase (α-Gal) enzyme, which is encoded by the melA gene hydrolyzes α-1,6 galactoside linkages found in sugars, such as raffinose and stachyose. These α-galacto-oligosaccharides (α-GOS), which are found in large quantities in vegetables, such as soy, can cause gastrointestinal disorders in sensitive individuals because monogastric animals (including humans) do not posses α-Gal in the gut. The use of microbial α-Gal is a promising alternative to eliminate α-GOS in soy-derived products. Using degenerate primers, the melA gene from Lactobacillus (L.) fermentum CRL722 was identified. The complete genomic sequence of melA (2223 bp), and of the genes flanking melA, were obtained using a combination of polymerase chain reaction-based techniques, and showed strong similarities with the α-Gal gene of thermophilic microorganisms. The α-Gal gene from L. fermentum CRL722 was cloned and the protein purified from cell-free extracts of the native and recombinant strains using various techniques (ion exchange chromatography, salt precipitation, sodium dodecyl sulfate-polyacrylamide gel electrophoresis, and ultra-filtration); Its main biochemical properties were determined. The enzyme was active at moderately high temperatures (55°C) and stable at wide ranges of temperatures and pH. The thermostable α-Gal from L. fermentum CRL722 could thus be used for technological applications, such as the removal of α-GOS found in soy products. The complete melA gene could also be inserted in other micro-organisms, that can survive in the harsh conditions of the gut to degrade α-GOS in situ. Both strategies would improve the overall acceptability of soy-derived products by improving their nutritional value. © 2006 Springer Science+Business Media, Inc.  
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application/pdf  
dc.language.iso
eng  
dc.publisher
Springer  
dc.rights
info:eu-repo/semantics/openAccess  
dc.rights.uri
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/  
dc.subject
No Corresponde  
dc.subject.classification
Inmunología  
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Medicina Básica  
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CIENCIAS MÉDICAS Y DE LA SALUD  
dc.title
A thermostable α-galactosidase from Lactobacillus fermentum CRL722: Genetic characterization and main properties  
dc.type
info:eu-repo/semantics/article  
dc.type
info:ar-repo/semantics/artículo  
dc.type
info:eu-repo/semantics/publishedVersion  
dc.date.updated
2017-08-24T18:59:04Z  
dc.journal.volume
53  
dc.journal.number
5  
dc.journal.pagination
374-378  
dc.journal.pais
Alemania  
dc.description.fil
Fil: Carrera Silva, Eugenio Antonio. Universidad Nacional de Córdoba; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucuman. Centro de Referencia Para Lactobacilos; Argentina  
dc.description.fil
Fil: Silvestroni, A.. University of Washington. School of Medicine; Estados Unidos. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucuman. Centro de Referencia Para Lactobacilos; Argentina  
dc.description.fil
Fil: Leblanc, Jean Guy Joseph. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucuman. Centro de Referencia Para Lactobacilos; Argentina  
dc.description.fil
Fil: Piard, J. C.. Institut National de la Recherche Agronomique; Francia  
dc.description.fil
Fil: Savoy, Graciela. Universidad Nacional de Tucumán; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucuman. Centro de Referencia Para Lactobacilos; Argentina  
dc.description.fil
Fil: Sesma, Fernando Juan Manuel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucuman. Centro de Referencia Para Lactobacilos; Argentina  
dc.journal.title
Current Microbiology  
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/doi/http://dx.doi.org/10.1007/s00284-005-0442-y