Mostrar el registro sencillo del ítem
dc.contributor.author
Zarate, Gabriela del Valle
![Se ha confirmado la validez de este valor de autoridad por un usuario](/themes/CONICETDigital/images/authority_control/invisible.gif)
dc.contributor.author
Nader, Maria Elena Fatima
![Se ha confirmado la validez de este valor de autoridad por un usuario](/themes/CONICETDigital/images/authority_control/invisible.gif)
dc.date.available
2018-02-15T19:36:23Z
dc.date.issued
2006-08
dc.identifier.citation
Zarate, Gabriela del Valle; Nader, Maria Elena Fatima; Viability and biological properties of probiotic vaginal lactobacilli after lyophilization and refrigerated storage into gelatin capsules; Elsevier; Process Biochemistry; 41; 8; 8-2006; 1779-1785
dc.identifier.issn
1359-5113
dc.identifier.uri
http://hdl.handle.net/11336/36578
dc.description.abstract
In the present study we investigated the survival and stability of probiotic properties of three vaginal lactobacilli after freeze drying with the addition of individual and combined compounds commonly used as pharmaceutical excipients and/or starters cryoprotectants, and subsequent refrigerated storage during 15 months. Lactobacilli, suspended in lactose, skim milk, ascorbic acid and combinations of them, were freeze-dried, packed into gelatin capsules and stored at 5 °C under darkness. At regular intervals throughout storage, freeze-dried samples were rehydrated and viability, adhesion to vaginal epithelial cells and antimicrobial activities were evaluated. The Lactobacillus tested conserved high viability up to 12 months in capsules containing ascorbic acid or combined excipients whereas lyophilization and storage with lactose or skim milk significantly decreased their survival. Abilities to produce lactic acid, H2O2 and bacteriocin were affected to different extents depending on the condition assayed. Lyophilization and storage also reduced the capacity of lactobacilli to adhere to vaginal epithelial cells but this property was partially restored after the first subculture in broth. The results obtained suggests that suitable selection of excipients that could also act as protecting agents of LAB during storage should be a valuable step in the development of a probiotic formulation with a stable shelf-life.
dc.format
application/pdf
dc.language.iso
eng
dc.publisher
Elsevier
![Se ha confirmado la validez de este valor de autoridad por un usuario](/themes/CONICETDigital/images/authority_control/invisible.gif)
dc.rights
info:eu-repo/semantics/openAccess
dc.rights.uri
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.subject
Lactobacillus
dc.subject
Lyophilization
dc.subject
Probiotic
dc.subject
Storage
dc.subject
Survival
dc.subject
Vagina
dc.subject.classification
Otras Ciencias Biológicas
![Se ha confirmado la validez de este valor de autoridad por un usuario](/themes/CONICETDigital/images/authority_control/invisible.gif)
dc.subject.classification
Ciencias Biológicas
![Se ha confirmado la validez de este valor de autoridad por un usuario](/themes/CONICETDigital/images/authority_control/invisible.gif)
dc.subject.classification
CIENCIAS NATURALES Y EXACTAS
![Se ha confirmado la validez de este valor de autoridad por un usuario](/themes/CONICETDigital/images/authority_control/invisible.gif)
dc.title
Viability and biological properties of probiotic vaginal lactobacilli after lyophilization and refrigerated storage into gelatin capsules
dc.type
info:eu-repo/semantics/article
dc.type
info:ar-repo/semantics/artículo
dc.type
info:eu-repo/semantics/publishedVersion
dc.date.updated
2017-08-24T18:59:29Z
dc.journal.volume
41
dc.journal.number
8
dc.journal.pagination
1779-1785
dc.journal.pais
Países Bajos
![Se ha confirmado la validez de este valor de autoridad por un usuario](/themes/CONICETDigital/images/authority_control/invisible.gif)
dc.journal.ciudad
Amsterdam
dc.description.fil
Fil: Zarate, Gabriela del Valle. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucuman. Centro de Referencia Para Lactobacilos; Argentina
dc.description.fil
Fil: Nader, Maria Elena Fatima. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucuman. Centro de Referencia Para Lactobacilos; Argentina
dc.journal.title
Process Biochemistry
![Se ha confirmado la validez de este valor de autoridad por un usuario](/themes/CONICETDigital/images/authority_control/invisible.gif)
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/doi/http://dx.doi.org/10.1016/j.procbio.2006.03.024
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/url/https://www.sciencedirect.com/science/article/pii/S135951130600122X
Archivos asociados