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dc.contributor.author
Bencke Malato, Marta
dc.contributor.author
Cabreira, Caroline
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Wiebke Strohm, Beatriz
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Bücker Neto, Lauro
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Mancini, Estefania
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Osorio, Marina B.
dc.contributor.author
Homrich, Milena S.
dc.contributor.author
Turchetto Zolet, Andreia C.
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De Carvalho, Mayra C.C.G.
dc.contributor.author
Stolf, Renata
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Weber, Ricardo L.M.
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Westergaard, Gastón
dc.contributor.author
Castagnaro, Atilio Pedro
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Abdelnoor, Ricardo V.
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Marcelino Guimarães, Francismar C.
dc.contributor.author
Margis Pinheiro, Márcia
dc.contributor.author
Bodanese Zanettini, Maria H.
dc.date.available
2018-02-15T19:35:13Z
dc.date.issued
2014-09
dc.identifier.citation
Bencke Malato, Marta; Cabreira, Caroline; Wiebke Strohm, Beatriz; Bücker Neto, Lauro; Mancini, Estefania; et al.; Genome-wide annotation of the soybean WRKY family and functional characterization of genes involved in response to Phakopsora pachyrhizi infection; BioMed Central; BMC Plant Biology; 14; 1; 9-2014; 1-18
dc.identifier.issn
1471-2229
dc.identifier.uri
http://hdl.handle.net/11336/36572
dc.description.abstract
Background: Many previous studies have shown that soybean WRKY transcription factors are involved in the plant response to biotic and abiotic stresses. Phakopsora pachyrhizi is the causal agent of Asian Soybean Rust, one of the most important soybean diseases. There are evidences that WRKYs are involved in the resistance of some soybean genotypes against that fungus. The number of WRKY genes already annotated in soybean genome was underrepresented. In the present study, a genome-wide annotation of the soybean WRKY family was carried out and members involved in the response to P. pachyrhizi were identified.Results: As a result of a soybean genomic databases search, 182 WRKY-encoding genes were annotated and 33 putative pseudogenes identified. Genes involved in the response to P. pachyrhizi infection were identified using superSAGE, RNA-Seq of microdissected lesions and microarray experiments. Seventy-five genes were differentially expressed during fungal infection. The expression of eight WRKY genes was validated by RT-qPCR. The expression of these genes in a resistant genotype was earlier and/or stronger compared with a susceptible genotype in response to P. pachyrhizi infection. Soybean somatic embryos were transformed in order to overexpress or silence WRKY genes. Embryos overexpressing a WRKY gene were obtained, but they were unable to convert into plants. When infected with P. pachyrhizi, the leaves of the silenced transgenic line showed a higher number of lesions than the wild-type plants.Conclusions: The present study reports a genome-wide annotation of soybean WRKY family. The participation of some members in response to P. pachyrhizi infection was demonstrated. The results contribute to the elucidation of gene function and suggest the manipulation of WRKYs as a strategy to increase fungal resistance in soybean plants.
dc.format
application/pdf
dc.language.iso
eng
dc.publisher
BioMed Central
dc.rights
info:eu-repo/semantics/openAccess
dc.rights.uri
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.subject
Asian Soybean Rust
dc.subject
Functional Analysis
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Fungus Resistance
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Genetic Transformation
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Glycine Max
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Transcription Factors
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Otras Biotecnología Agropecuaria
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Biotecnología Agropecuaria
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CIENCIAS AGRÍCOLAS
dc.title
Genome-wide annotation of the soybean WRKY family and functional characterization of genes involved in response to Phakopsora pachyrhizi infection
dc.type
info:eu-repo/semantics/article
dc.type
info:ar-repo/semantics/artículo
dc.type
info:eu-repo/semantics/publishedVersion
dc.date.updated
2017-12-11T19:39:56Z
dc.journal.volume
14
dc.journal.number
1
dc.journal.pagination
1-18
dc.journal.pais
Reino Unido
dc.journal.ciudad
Londres
dc.description.fil
Fil: Bencke Malato, Marta. Universidade Federal do Rio Grande do Sul; Brasil
dc.description.fil
Fil: Cabreira, Caroline. Universidade Federal do Rio Grande do Sul; Brasil
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Fil: Wiebke Strohm, Beatriz. Universidade Federal do Rio Grande do Sul; Brasil
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Fil: Bücker Neto, Lauro. Universidade Federal do Rio Grande do Sul; Brasil
dc.description.fil
Fil: Mancini, Estefania. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Rosario. Instituto de Agrobiotecnología de Rosario; Argentina
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Fil: Osorio, Marina B.. Universidade Federal do Rio Grande do Sul; Brasil
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Fil: Homrich, Milena S.. Universidade Federal do Rio Grande do Sul; Brasil
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Fil: Turchetto Zolet, Andreia C.. Universidade Federal do Rio Grande do Sul; Brasil
dc.description.fil
Fil: De Carvalho, Mayra C.C.G.. Ministerio da Agricultura Pecuaria e Abastecimento de Brasil. Empresa Brasileira de Pesquisa Agropecuaria; Brasil
dc.description.fil
Fil: Stolf, Renata. Ministerio da Agricultura Pecuaria e Abastecimento de Brasil. Empresa Brasileira de Pesquisa Agropecuaria; Brasil
dc.description.fil
Fil: Weber, Ricardo L.M.. Universidade Federal do Rio Grande do Sul; Brasil
dc.description.fil
Fil: Westergaard, Gastón. Instituto de Agrobiotecnología de Rosario (indear); Argentina
dc.description.fil
Fil: Castagnaro, Atilio Pedro. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Instituto de Tecnología Agroindustrial del Noroeste Argentino. Provincia de Tucumán. Ministerio de Desarrollo Productivo. Estación Experimental Agroindustrial ; Argentina
dc.description.fil
Fil: Abdelnoor, Ricardo V.. Ministerio da Agricultura Pecuaria e Abastecimento de Brasil. Empresa Brasileira de Pesquisa Agropecuaria; Brasil
dc.description.fil
Fil: Marcelino Guimarães, Francismar C.. Ministerio da Agricultura Pecuaria e Abastecimento de Brasil. Empresa Brasileira de Pesquisa Agropecuaria; Brasil
dc.description.fil
Fil: Margis Pinheiro, Márcia. Universidade Federal do Rio Grande do Sul; Brasil
dc.description.fil
Fil: Bodanese Zanettini, Maria H.. Universidade Federal do Rio Grande do Sul; Brasil
dc.journal.title
BMC Plant Biology
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/url/https://bmcplantbiol.biomedcentral.com/articles/10.1186/s12870-014-0236-0
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/doi/http://dx.doi.org/10.1186/s12870-014-0236-0
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