Artículo

PCR-based prediction of type I β-exotoxin production in Bacillus thuringiensis strains

Sauka, Diego HermanIcon ; Perez, Melisa PaulaIcon ; Lopez, Nanci N.; Onco, María InésIcon ; Berretta, Marcelo FacundoIcon ; Benintende, Graciela Beatriz
Fecha de publicación: 10/2014
Editorial: Academic Press Inc Elsevier Science
Revista: Journal of Invertebrate Pathology
ISSN: 0022-2011
Idioma: Inglés
Tipo de recurso: Artículo publicado
Clasificación temática:

Resumen

Some Bacillus thuringiensis strains secrete type I β-exotoxin, which is a non-specific insecticidal and thermostable adenine nucleoside oligosaccharide. Toxicity bioassays and HPLC are traditional methods for detecting β-exotoxin. With the aim of establish a first rapid approach for prediction of type I β-exotoxin production, two PCR-based methods were successfully evaluated in B. thuringiensis strains and native isolates. In order to validate a reliable technology, results obtained by this method were correlated with that obtained from Musca domestica bioassays.
Palabras clave: Bacillus Thuringiensis , Β-Exotoxin , Thue Gene , Pcr
 
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info:eu-repo/semantics/openAccess Excepto donde se diga explícitamente, este item se publica bajo la siguiente descripción: Creative Commons Attribution-NonCommercial-ShareAlike 2.5 Unported (CC BY-NC-SA 2.5)
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URI: http://hdl.handle.net/11336/36540
DOI: http://dx.doi.org/10.1016/j.jip.2014.08.001
URL: https://www.sciencedirect.com/science/article/pii/S002220111400113X
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Citación
Sauka, Diego Herman; Perez, Melisa Paula; Lopez, Nanci N.; Onco, María Inés; Berretta, Marcelo Facundo; et al.; PCR-based prediction of type I β-exotoxin production in Bacillus thuringiensis strains; Academic Press Inc Elsevier Science; Journal of Invertebrate Pathology; 122; 10-2014; 28-31
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