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dc.contributor.author
Montanari, Sonia
dc.contributor.author
Wang, Xing-Hua
dc.contributor.author
Yannarelli, Gustavo Gabriel
dc.contributor.author
Dayan, Victor
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Berger, Thorsten
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Zocche, Larissa
dc.contributor.author
Kobayashi, Eiji
dc.contributor.author
Viswanathan, Sowmya
dc.contributor.author
Keating, Armand
dc.date.available
2018-02-15T14:14:50Z
dc.date.issued
2014-10
dc.identifier.citation
Montanari, Sonia; Wang, Xing-Hua; Yannarelli, Gustavo Gabriel; Dayan, Victor; Berger, Thorsten; et al.; Development and characterization of a new inbred transgenic rat strain expressing DsRed monomeric fluorescent protein; Springer; Transgenic Research; 23; 5; 10-2014; 779-793
dc.identifier.issn
0962-8819
dc.identifier.uri
http://hdl.handle.net/11336/36511
dc.description.abstract
The inbred rat is a suitable model for studying human disease and because of its larger size is more amenable to complex surgical manipulation than the mouse. While the rodent fulfills many of the criteria for transplantation research, an important requirement is the ability to mark and track donors cells and assess organ viability. However, tracking ability is limited by the availability of transgenic (Tg) rats that express suitable luminescent or fluorescent proteins. Red fluorescent protein cloned from Discosoma coral (DsRed) has several advantages over other fluorescent proteins, including in vivo detection in the whole animal and ex vivo visualization in organs as there is no interference with autofluorescence. We generated and characterized a novel inbred Tg Lewis rat strain expressing DsRed monomeric (DsRed mono) fluorescent protein under the control of a ubiquitously expressed ROSA26 promoter. DsRed mono Tg rats ubiquitously expressed the marker gene as detected by RT-PCR but the protein was expressed at varying levels in different organs. Conventional skin grafting experiments showed acceptance of DsRed monomeric Tg rat skin on wild-type rats for more than 30 days. Cardiac transplantation of DsRed monomeric Tg rat hearts into wild-type recipients further showed graft acceptance and long-term organ viability (>6 months). The DsRed monomeric Tg rat provides marked cells and/or organs that can be followed for long periods without immune rejection and therefore is a suitable model to investigate cell tracking and organ transplantation
dc.format
application/pdf
dc.language.iso
eng
dc.publisher
Springer
dc.rights
info:eu-repo/semantics/restrictedAccess
dc.rights.uri
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.subject
Mesenchymal Stromal Cells
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Dsred Monomeric Protein
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Heterotopic Heart Transplantation
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Immunogenicity
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Skin Grafting
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Transgenic Rat
dc.subject.classification
Otras Biotecnologías de la Salud
dc.subject.classification
Biotecnología de la Salud
dc.subject.classification
CIENCIAS MÉDICAS Y DE LA SALUD
dc.title
Development and characterization of a new inbred transgenic rat strain expressing DsRed monomeric fluorescent protein
dc.type
info:eu-repo/semantics/article
dc.type
info:ar-repo/semantics/artículo
dc.type
info:eu-repo/semantics/publishedVersion
dc.date.updated
2018-02-14T19:28:13Z
dc.journal.volume
23
dc.journal.number
5
dc.journal.pagination
779-793
dc.journal.pais
Alemania
dc.journal.ciudad
Berlin
dc.description.fil
Fil: Montanari, Sonia. University Health Network. Toronto; Canadá. University of Toronto; Canadá. Princess Margaret Cancer Centre. Toronto; Canadá
dc.description.fil
Fil: Wang, Xing-Hua. University Health Network. Toronto; Canadá
dc.description.fil
Fil: Yannarelli, Gustavo Gabriel. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. University Health Network. Toronto; Canadá
dc.description.fil
Fil: Dayan, Victor. University Health Network. Toronto; Canadá
dc.description.fil
Fil: Berger, Thorsten. University Health Network. Toronto; Canadá
dc.description.fil
Fil: Zocche, Larissa. University Health Network. Toronto; Canadá
dc.description.fil
Fil: Kobayashi, Eiji. Jichi Medical School. Tochigi; Japón
dc.description.fil
Fil: Viswanathan, Sowmya. University Health Network. Toronto; Canadá
dc.description.fil
Fil: Keating, Armand. University of Toronto; Canadá. University Health Network. Toronto; Canadá
dc.journal.title
Transgenic Research
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/doi/http://dx.doi.org/10.1007/s11248-014-9814-z
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/url/https://link.springer.com/article/10.1007%2Fs11248-014-9814-z