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dc.contributor.author Sepúlveda, Diana Elizabeth
dc.contributor.author Cabeza Meckert, Patricia
dc.contributor.author Locatelli, Paola
dc.contributor.author Olea, Fernanda Daniela
dc.contributor.author Perez, Nestor Gustavo
dc.contributor.author Pinilla, Oscar Andres
dc.contributor.author Diaz, Romina Gisel
dc.contributor.author Crottogini, Alberto José
dc.contributor.author Laguens, Rubén
dc.date.available 2018-02-14T18:27:08Z
dc.date.issued 2014-11
dc.identifier.citation Sepúlveda, Diana Elizabeth; Cabeza Meckert, Patricia; Locatelli, Paola; Olea, Fernanda Daniela; Perez, Nestor Gustavo; et al.; Activated macrophages as a feeder layer for growth of resident cardiac progenitor cells; Springer; Cytotechnology (dordrecht); 68; 4; 11-2014; 665-674
dc.identifier.issn 0920-9069
dc.identifier.uri http://hdl.handle.net/11336/36443
dc.description.abstract The adult heart contains a population of cardiac progenitor cells (CPCs). Growing and collecting an adequate number of CPCs demands complex culture media containing growth factors. Since activated macrophages secrete many growth factors, we investigated if activated isolated heart cells seeded on a feeder layer of activated peritoneal macrophages (PM) could result in CPCs and if these, in turn, could exert cardioprotection in rats with myocardial infarction (MI). Heart cells of inbred Wistar rats were isolated by collagenase digestion and cultured on PM obtained 72 h after intraperitoneal injection of 12 ml thioglycollate. Cells (1 × 106) exhibiting CPC phenotype (immunohistochemistry) were injected in the periphery of rat MI 10 min after coronary artery occlusion. Control rats received vehicle. Three weeks later, left ventricular (LV) function (echocardiogram) was assessed, animals were euthanized and the hearts removed for histological studies. Five to six days after seeding heart cells on PM, spherical clusters composed of small bright and spherical cells expressing mostly c-Kit and Sca-1 antigens were apparent. After explant, those clusters developed cobblestone-like monolayers that expressed smooth muscle actin and sarcomeric actin and were successfully transferred for more than ten passages. When injected in the MI periphery, many of them survived at 21 days after coronary ligature, improved LV ejection fraction and decreased scar size as compared with control rats. CPC-derived cells with cardiocyte and smooth muscle phenotypes can be successfully grown on a feeder layer of activated syngeneic PM. These cells decreased scar size and improved heart function in rats with MI.
dc.format application/pdf
dc.language.iso eng
dc.publisher Springer
dc.rights info:eu-repo/semantics/restrictedAccess
dc.rights.uri https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.subject Cardiac progenitor cells
dc.subject Macrophage
dc.subject Myocardial infarction
dc.subject Rat
dc.subject.classification Otras Ciencias Biológicas
dc.subject.classification Ciencias Biológicas
dc.subject.classification CIENCIAS NATURALES Y EXACTAS
dc.title Activated macrophages as a feeder layer for growth of resident cardiac progenitor cells
dc.type info:eu-repo/semantics/article
dc.type info:ar-repo/semantics/artículo
dc.type info:eu-repo/semantics/publishedVersion
dc.date.updated 2018-02-14T13:19:42Z
dc.journal.volume 68
dc.journal.number 4
dc.journal.pagination 665-674
dc.journal.pais Alemania
dc.journal.ciudad Berlin
dc.description.fil Fil: Sepúlveda, Diana Elizabeth. Universidad Favaloro; Argentina
dc.description.fil Fil: Cabeza Meckert, Patricia. Universidad Favaloro; Argentina
dc.description.fil Fil: Locatelli, Paola. Universidad Favaloro; Argentina
dc.description.fil Fil: Olea, Fernanda Daniela. Universidad Favaloro; Argentina
dc.description.fil Fil: Perez, Nestor Gustavo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - la Plata. Centro de Investigaciones Cardiovasculares "Dr. Horacio Eugenio Cingolani". Universidad Nacional de la Plata. Facultad de Ciencias Médicas. Centro de Investigaciones Cardiovasculares "Dr. Horacio Eugenio Cingolani"; Argentina
dc.description.fil Fil: Pinilla, Oscar Andres. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - la Plata. Centro de Investigaciones Cardiovasculares "Dr. Horacio Eugenio Cingolani". Universidad Nacional de la Plata. Facultad de Ciencias Médicas. Centro de Investigaciones Cardiovasculares "Dr. Horacio Eugenio Cingolani"; Argentina
dc.description.fil Fil: Diaz, Romina Gisel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - la Plata. Centro de Investigaciones Cardiovasculares "Dr. Horacio Eugenio Cingolani". Universidad Nacional de la Plata. Facultad de Ciencias Médicas. Centro de Investigaciones Cardiovasculares "Dr. Horacio Eugenio Cingolani"; Argentina
dc.description.fil Fil: Crottogini, Alberto José. Universidad Favaloro; Argentina
dc.description.fil Fil: Laguens, Rubén. Universidad Favaloro; Argentina
dc.journal.title Cytotechnology (dordrecht)
dc.relation.alternativeid info:eu-repo/semantics/altIdentifier/doi/http://dx.doi.org/10.1007/s10616-014-9814-0
dc.relation.alternativeid info:eu-repo/semantics/altIdentifier/url/https://link.springer.com/article/10.1007%2Fs10616-014-9814-0
dc.conicet.fuente individual


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info:eu-repo/semantics/restrictedAccess Excepto donde se diga explícitamente, este item se publica bajo la siguiente descripción: Creative Commons Attribution-NonCommercial-ShareAlike 2.5 Unported (CC BY-NC-SA 2.5)