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dc.contributor.author
Garbarino Azúa, Darío Jose  
dc.contributor.author
Saucedo, Lucia  
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Giordana, S.  
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Magri, M.L.  
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Buffone, Mariano Gabriel  
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Neuspiller, F.  
dc.contributor.author
Vazquez, Monica Hebe  
dc.contributor.author
Marin Briggiler, Clara Isabel  
dc.date.available
2018-02-12T15:51:25Z  
dc.date.issued
2017-09  
dc.identifier.citation
Garbarino Azúa, Darío Jose; Saucedo, Lucia; Giordana, S.; Magri, M.L.; Buffone, Mariano Gabriel; et al.; Fibroblast growth factor 2 (FGF2) is present in human spermatozoa and is related with sperm motility: the use of recombinant FGF2 to improve motile sperm recovery; Wiley-liss, Div John Wiley & Sons Inc; Andrology; 5; 5; 9-2017; 990-998  
dc.identifier.issn
2047-2919  
dc.identifier.uri
http://hdl.handle.net/11336/36420  
dc.description.abstract
Fibroblast growth factors (FGFs) and their receptors (FGFRs) regulate several functions of somatic cells. In a previous work, we reported FGFR expression in human spermatozoa and their involvement in motility. This study aimed to evaluate the presence and localization of fibroblast growth factor 2 (FGF2) in human spermatozoa, to determine the relationship of FGF2 levels with conventional semen parameters and to assess the effect of recombinant FGF2 (rFGF2) on sperm recovery in a selection procedure. Western immunoblotting analysis using an antibody against FGF2 revealed an 18-kDa band in sperm protein extracts. The protein was immunolocalized in the sperm flagellum and acrosomal region, as well as in all germ cells. Sperm FGF2 levels, assessed by flow cytometry, showed a positive (p < 0.05) correlation with sperm concentration, motility, total sperm number and total motile cells per ejaculate. Moreover, samples with abnormal motility depicted diminished (p < 0.01) FGF2 levels compared to those with normal motility. Spermatozoa exposed to rFGF2 bound the protein, exhibited higher (p < 0.05) total and motile sperm recoveries, and increased (p < 0.01) kinematic parameters after the swim-up. Findings herein presented lead to consider sperm FGF2 level as a potential marker of sperm quality, and rFGF2 as a supplement for improving sperm recovery in selection techniques.  
dc.format
application/pdf  
dc.language.iso
eng  
dc.publisher
Wiley-liss, Div John Wiley & Sons Inc  
dc.rights
info:eu-repo/semantics/openAccess  
dc.rights.uri
https://creativecommons.org/licenses/by-nc-nd/2.5/ar/  
dc.subject
Fgf2  
dc.subject
Fibroblast Growth Factor 2  
dc.subject
Motility  
dc.subject
Spermatozoa  
dc.subject
Swim-Up  
dc.subject.classification
Biología Reproductiva  
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Ciencias Biológicas  
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CIENCIAS NATURALES Y EXACTAS  
dc.title
Fibroblast growth factor 2 (FGF2) is present in human spermatozoa and is related with sperm motility: the use of recombinant FGF2 to improve motile sperm recovery  
dc.type
info:eu-repo/semantics/article  
dc.type
info:ar-repo/semantics/artículo  
dc.type
info:eu-repo/semantics/publishedVersion  
dc.date.updated
2018-02-05T13:42:47Z  
dc.identifier.eissn
2047-2927  
dc.journal.volume
5  
dc.journal.number
5  
dc.journal.pagination
990-998  
dc.journal.pais
Reino Unido  
dc.description.fil
Fil: Garbarino Azúa, Darío Jose. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biología y Medicina Experimental. Fundación de Instituto de Biología y Medicina Experimental. Instituto de Biología y Medicina Experimental; Argentina  
dc.description.fil
Fil: Saucedo, Lucia. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biología y Medicina Experimental. Fundación de Instituto de Biología y Medicina Experimental. Instituto de Biología y Medicina Experimental; Argentina  
dc.description.fil
Fil: Giordana, S.. Instituto Valenciano de Infertilidad ; Argentina  
dc.description.fil
Fil: Magri, M.L.. Instituto Valenciano de Infertilidad ; Argentina  
dc.description.fil
Fil: Buffone, Mariano Gabriel. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biología y Medicina Experimental. Fundación de Instituto de Biología y Medicina Experimental. Instituto de Biología y Medicina Experimental; Argentina  
dc.description.fil
Fil: Neuspiller, F.. Instituto Valenciano de Infertilidad ; Argentina  
dc.description.fil
Fil: Vazquez, Monica Hebe. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biología y Medicina Experimental. Fundación de Instituto de Biología y Medicina Experimental. Instituto de Biología y Medicina Experimental; Argentina  
dc.description.fil
Fil: Marin Briggiler, Clara Isabel. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biología y Medicina Experimental. Fundación de Instituto de Biología y Medicina Experimental. Instituto de Biología y Medicina Experimental; Argentina  
dc.journal.title
Andrology  
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/url/http://onlinelibrary.wiley.com/doi/10.1111/andr.12398/abstract  
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/doi/http://dx.doi.org/10.1111/andr.1239  
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/pmid/28732140