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dc.contributor.author Gonzalez Deniselle, Maria Claudia
dc.contributor.author López Costa, Juan Jose
dc.contributor.author Pecci Saavedra, Jorge
dc.contributor.author Pietranera, Luciana
dc.contributor.author Gonzalez, Susana Laura
dc.contributor.author Garay, Laura Ines
dc.contributor.author Guennoun, Rachida
dc.contributor.author Schumacher, Michael
dc.contributor.author de Nicola, Alejandro Federico
dc.date.available 2018-02-11T22:34:30Z
dc.date.issued 2002
dc.identifier.citation Gonzalez Deniselle, Maria Claudia; López Costa, Juan Jose; Pecci Saavedra, Jorge; Pietranera, Luciana; Gonzalez, Susana Laura; et al.; Progesterone neuroprotection in the Wobbler mouse, a genetic model of spinal cord motor neuron disease; Elsevier Science; Neurobiology of Disease; 11; 3; 2002; 457-468
dc.identifier.issn 0969-9961
dc.identifier.uri http://hdl.handle.net/11336/36412
dc.description.abstract Motor neuron degeneration characterizes the spinal cord of patients with amyotrophic lateral sclerosis and the Wobbler mouse mutant. Considering that progesterone (PROG) provides neuroprotection in experimental ischemia and injury, its potential role in neurodegeneration was studied in the murine model. Two-month-old symptomatic Wobbler mice were left untreated or received sc a 20-mg PROG implant for 15 days. Both light and electron microscopy of Wobbler mice spinal cord showed severely affected motor neurons with profuse cytoplasmic vacuolation of the endoplasmic reticulum and/or Golgi apparatus and ruptured mitochondria with damaged cristae, a profile indicative of a type II cytoplasmic form of cell death. In contrast to untreated mice, neuropathology was less severe in Wobbler mice receiving PROG; including a reduction of vacuolation and of the number of vacuolated cells and better conservation of the mitochondrial ultrastructure. In biochemical studies, we determined the mRNA for the α3 subunit of Na,K-ATPase, a neuronal enzyme controlling ion fluxes, neurotransmission, membrane potential, and nutrient uptake. In untreated Wobbler mice, mRNA levels in motor neurons were reduced by half compared to controls, whereas PROG treatment of Wobbler mice restored the expression of α3 subunit Na,K-ATPase mRNA. Therefore, PROG was able to rescue motor neurons from degeneration, based on recovery of histopathological abnormalities and of mRNA levels of the sodium pump. However, because the gene mutation in Wobbler mice is still unknown, further studies are needed to unveil the action of PROG and the mechanism of neuronal death in this genetic model of neurodegeneration.
dc.format application/pdf
dc.language.iso eng
dc.publisher Elsevier Science
dc.rights info:eu-repo/semantics/restrictedAccess
dc.rights.uri https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.subject NEUROPROTECTION
dc.subject SPINAL CORD
dc.subject PROGESTERONE
dc.subject NEURODEGENERATION
dc.subject WOBBLER MICE
dc.subject AMYOTROPHIC LATERAL SCLEROSIS
dc.subject.classification Neurociencias
dc.subject.classification Medicina Básica
dc.subject.classification CIENCIAS MÉDICAS Y DE LA SALUD
dc.title Progesterone neuroprotection in the Wobbler mouse, a genetic model of spinal cord motor neuron disease
dc.type info:eu-repo/semantics/article
dc.type info:ar-repo/semantics/artículo
dc.type info:eu-repo/semantics/publishedVersion
dc.date.updated 2017-12-28T17:49:44Z
dc.journal.volume 11
dc.journal.number 3
dc.journal.pagination 457-468
dc.journal.pais Estados Unidos
dc.description.fil Fil: Gonzalez Deniselle, Maria Claudia. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biología y Medicina Experimental. Fundación de Instituto de Biología y Medicina Experimental. Instituto de Biología y Medicina Experimental; Argentina. Universidad de Buenos Aires. Facultad de Medicina. Departamento de Bioquímica Humana; Argentina
dc.description.fil Fil: López Costa, Juan Jose. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Biología Celular y Neurociencia ; Argentina
dc.description.fil Fil: Pecci Saavedra, Jorge. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Biología Celular y Neurociencia ; Argentina
dc.description.fil Fil: Pietranera, Luciana. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biología y Medicina Experimental. Fundación de Instituto de Biología y Medicina Experimental. Instituto de Biología y Medicina Experimental; Argentina. Universidad de Buenos Aires. Facultad de Medicina. Departamento de Bioquímica Humana; Argentina
dc.description.fil Fil: Gonzalez, Susana Laura. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biología y Medicina Experimental. Fundación de Instituto de Biología y Medicina Experimental. Instituto de Biología y Medicina Experimental; Argentina. Universidad de Buenos Aires. Facultad de Medicina. Departamento de Bioquímica Humana; Argentina
dc.description.fil Fil: Garay, Laura Ines. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biología y Medicina Experimental. Fundación de Instituto de Biología y Medicina Experimental. Instituto de Biología y Medicina Experimental; Argentina. Universidad de Buenos Aires. Facultad de Medicina. Departamento de Bioquímica Humana; Argentina
dc.description.fil Fil: Guennoun, Rachida. Inserm; Francia
dc.description.fil Fil: Schumacher, Michael. Inserm; Francia
dc.description.fil Fil: de Nicola, Alejandro Federico. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biología y Medicina Experimental. Fundación de Instituto de Biología y Medicina Experimental. Instituto de Biología y Medicina Experimental; Argentina. Universidad de Buenos Aires. Facultad de Medicina. Departamento de Bioquímica Humana; Argentina
dc.journal.title Neurobiology of Disease
dc.relation.alternativeid info:eu-repo/semantics/altIdentifier/url/https://www.sciencedirect.com/science/article/pii/S0969996102905644
dc.relation.alternativeid info:eu-repo/semantics/altIdentifier/doi/http://dx.doi.org/10.1006/nbdi.2002.0564
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  • Articulos(IBCN) [211]
    Articulos de INST.DE BIOLO.CEL.Y NEURCS."PROF.E.DE ROBERTIS"
  • Articulos(IBYME) [1050]
    Articulos de INST.DE BIOLOGIA Y MEDICINA EXPERIMENTAL (I)

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info:eu-repo/semantics/restrictedAccess Excepto donde se diga explícitamente, este item se publica bajo la siguiente descripción: Creative Commons Attribution-NonCommercial-ShareAlike 2.5 Unported (CC BY-NC-SA 2.5)