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dc.contributor.author
Rozenblum, Tomas Guido
dc.contributor.author
Kaufman, Tomás
dc.contributor.author
Vitullo, Alfredo Daniel
dc.date.available
2018-02-09T18:39:50Z
dc.date.issued
2014-01
dc.identifier.citation
Rozenblum, Tomas Guido; Kaufman, Tomás; Vitullo, Alfredo Daniel; Myelin Basic Protein and a Multiple Sclerosis-related MBP-peptide Bind to Oligonucleotides; The American Society of Gene & Cell Therapy; Molecular Therapy-Nucleic Acids; 3; 1-2014; 1-9; e192
dc.identifier.issn
2162-2531
dc.identifier.uri
http://hdl.handle.net/11336/36348
dc.description.abstract
Aptamer ligands for myelin basic protein (MBP) were obtained using the systematic evolution of ligand by exponential enrichment (SELEX) method. Two clones were isolated from a pool of oligonucleotides and tested for MBP targeting. Using purified MBP, we demonstrated the binding activity of the aptamers and we also showed the affinity of MBP for oligonucleotides of specific length. Moreover, one selected aptamer competitively inhibited the binding of an MBP-specific antibody to MBP and the aptamer was found more sensitive than a commercial antibody. In addition, we showed the ability of the aptamer to detect myelin-rich regions in paraffin-embedded mouse brain tissue. Therefore, the MBP-binding activity of the selected oligonucleotide may prove useful as a tool for life science and medical research for myelin detection and might be a good lead for testing it in autoimmune diseases such as multiple sclerosis.
dc.format
application/pdf
dc.language.iso
eng
dc.publisher
The American Society of Gene & Cell Therapy
dc.rights
info:eu-repo/semantics/openAccess
dc.rights.uri
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.subject
Aptamers
dc.subject
Histochemistry
dc.subject
Mltiple Sclerosis
dc.subject
Myelin Basic Protein
dc.subject.classification
Otras Biotecnologías de la Salud
dc.subject.classification
Biotecnología de la Salud
dc.subject.classification
CIENCIAS MÉDICAS Y DE LA SALUD
dc.title
Myelin Basic Protein and a Multiple Sclerosis-related MBP-peptide Bind to Oligonucleotides
dc.type
info:eu-repo/semantics/article
dc.type
info:ar-repo/semantics/artículo
dc.type
info:eu-repo/semantics/publishedVersion
dc.date.updated
2018-02-08T21:12:57Z
dc.journal.volume
3
dc.journal.pagination
1-9; e192
dc.journal.pais
Estados Unidos
dc.description.fil
Fil: Rozenblum, Tomas Guido. Universidad Maimonides. Facultad de Ciencias de la Salud; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
dc.description.fil
Fil: Kaufman, Tomás. Universidad Maimonides. Facultad de Ciencias de la Salud; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
dc.description.fil
Fil: Vitullo, Alfredo Daniel. Universidad Maimonides. Facultad de Ciencias de la Salud; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
dc.journal.title
Molecular Therapy-Nucleic Acids
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/doi/http://dx.doi.org/10.1038/mtna.2014.43
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/url/https://www.sciencedirect.com/science/article/pii/S2162253116303316


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