Artículo
B Chromosomes in the Tree Frog Hypsiboas albopunctatus (Anura: Hylidae)
Ferro, Juan Martín
; Marti, Dardo Andrea
; Bidau, Claudio Juan
; Suarez, Pablo
; Nagamachi, Cleusa Y.; Pieczarka, Júlio C.; Baldo, Juan Diego
Fecha de publicación:
12/2012
Editorial:
Herpetologists League
Revista:
Herpetologica
ISSN:
0018-0831
Idioma:
Inglés
Tipo de recurso:
Artículo publicado
Clasificación temática:
Resumen
Supernumerary or B chromosomes are one of the main causes for numerical chromosomal variation in higher eukaryotes. These extragenetic elements have been studied for more than a century, with the goal of trying to understand their origin, and how they survive as a polymorphism in natural populations. Hypsiboas albopunctatus is a nocturnal hylid frog distributed in the central–eastern part of South America. Previously, variation in chromosome numbers was described for a population from Rio Claro, São Paulo, Brazil, in which a single small-sized, metacentric B chromosome was present in ca. 40% of the analyzed individuals (n = 17). We herein describe the presence of B chromosomes in populations of H. albopunctatus from northeastern Argentina (Corrientes and Misiones provinces), with unusual morphological and structural characteristics. The frequency of B chromosomes varied significantly among analyzed populations. We found four diploid numbers (2N = 22, 22 + 1B, 22 + 2B, and 22 + 3B), and in a few individuals mitotic instability occurred. C banding revealed variations in the heterochromatin (DAPI+) pattern between Bs with similar morphology, indicating the existence of two new structural variants of these supernumerary elements in H. albopunctatus (B1 and B2). Nucleolar organizer regions marked positively on the eighth pair, coincident with the location of ribosomal DNA as demonstrated with fluorescent in situ hybridization, but Bs did not mark positive with these two techniques. Also, fluorescent in situ hybridization with telomeric probes showed no differences in location and intensity between Bs and autosomal chromosomes. The present communication is the first case of B chromosome polymorphisms in hylid frogs and the sixth reported in Anura.
Palabras clave:
Fish
,
Hypsiboas Albopunctatus
,
Polymorphism
,
Supernumerary Chromosome
Archivos asociados
Licencia
Identificadores
Colecciones
Articulos(CCT - NORDESTE)
Articulos de CTRO.CIENTIFICO TECNOL.CONICET - NORDESTE
Articulos de CTRO.CIENTIFICO TECNOL.CONICET - NORDESTE
Articulos(IBS)
Articulos de INSTITUTO DE BIOLOGIA SUBTROPICAL
Articulos de INSTITUTO DE BIOLOGIA SUBTROPICAL
Citación
Ferro, Juan Martín; Marti, Dardo Andrea; Bidau, Claudio Juan; Suarez, Pablo; Nagamachi, Cleusa Y.; et al.; B Chromosomes in the Tree Frog Hypsiboas albopunctatus (Anura: Hylidae); Herpetologists League; Herpetologica; 68; 4; 12-2012; 482-490
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