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dc.contributor.author Wang, Xing Jia
dc.contributor.author Dyson, Matthew T.
dc.contributor.author Mondillo, Carolina
dc.contributor.author Patrignani, Zoraida
dc.contributor.author Pignataro, Omar Pedro
dc.contributor.author Stocco, Douglas M.
dc.date.available 2018-02-08T21:09:04Z
dc.date.issued 2002-02-25
dc.identifier.citation Wang, Xing Jia; Dyson, Matthew T.; Mondillo, Carolina; Patrignani, Zoraida; Pignataro, Omar Pedro; et al.; Interaction between arachidonic acid and cAMP signaling pathways enhance steroidogenesis and StAR gene expression in MA-10 Leydig tumor cells; Elsevier Ireland; Molecular and Cellular Endocrinology; 188; 1; 25-2-2002; 55-63
dc.identifier.issn 0303-7207
dc.identifier.uri http://hdl.handle.net/11336/36241
dc.description.abstract Previous studies have demonstrated that trophic hormone stimulation induced cyclic AMP (cAMP) formation and arachidonic acid (AA) release from phospholipids and that both these compounds were required for steroid biosynthesis and steroidogenic acute regulatory (StAR) gene expression in MA-10 mouse Leydig tumor cells. The present study further investigates the synergistic effects of the AA and cAMP interaction on steroidogenesis. To demonstrate cAMP-induced AA release, MA-10 cells were pre-loaded with 3H-AA and subsequently treated with dibutyryl cyclic AMP (dbcAMP). Stimulation with dbcAMP significantly induced AA release in MA-10 cells to a level 145.7% higher than that of controls. Lowering intracellular cAMP concentration by expressing a cAMP-phosphodiesterase significantly reduced human chorionic gonadotrophin (hCG)-induced AA release. The dbcAMP-induced AA release was inhibited significantly by the phospholipase A(2) (PLA(2)) inhibitor dexamethasone (Dex) and also by the protein kinase A (PKA) inhibitor H89, suggesting the involvement of PKA phosphorylation and/or PLA(2) activation in cAMP-induced AA release. The effect of the interaction between AA and cAMP on StAR gene expression and steroid production was also investigated. While 0.2 mM dbcAMP induced only very low levels of StAR protein, StAR mRNA, StAR promoter activity and steroid production, all of these parameters increased dramatically as AA concentration in the culture medium was increased from 0 to 200 microM. Importantly, AA was not able to induce a significant increase in steroidogenesis at any concentration when used in the absence of dbcAMP. However, when used in concert with submaximal concentrations of dbcAMP (0.05 mm to 0.5 mm), AA was capable of stimulating StAR gene expression and increasing steroid production significantly. The results from this study demonstrate that AA and cAMP act in a highly synergistic manner to increase the sensitivity of steroid production to trophic hormone stimulation and probably do so by increasing StAR gene expression.
dc.format application/pdf
dc.language.iso eng
dc.publisher Elsevier Ireland
dc.rights info:eu-repo/semantics/openAccess
dc.rights.uri https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.subject.classification Bioquímica y Biología Molecular
dc.subject.classification Ciencias Biológicas
dc.subject.classification CIENCIAS NATURALES Y EXACTAS
dc.subject.classification Patología
dc.subject.classification Medicina Básica
dc.subject.classification CIENCIAS MÉDICAS Y DE LA SALUD
dc.title Interaction between arachidonic acid and cAMP signaling pathways enhance steroidogenesis and StAR gene expression in MA-10 Leydig tumor cells
dc.type info:eu-repo/semantics/article
dc.type info:ar-repo/semantics/artículo
dc.type info:eu-repo/semantics/publishedVersion
dc.date.updated 2017-12-28T13:41:30Z
dc.identifier.eissn 1872-8057
dc.journal.volume 188
dc.journal.number 1
dc.journal.pagination 55-63
dc.journal.pais Irlanda
dc.journal.ciudad Limerick
dc.description.fil Fil: Wang, Xing Jia. University of Texas; Estados Unidos
dc.description.fil Fil: Dyson, Matthew T.. University of Texas; Estados Unidos
dc.description.fil Fil: Mondillo, Carolina. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biología y Medicina Experimental. Fundación de Instituto de Biología y Medicina Experimental. Instituto de Biología y Medicina Experimental; Argentina
dc.description.fil Fil: Patrignani, Zoraida. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biología y Medicina Experimental. Fundación de Instituto de Biología y Medicina Experimental. Instituto de Biología y Medicina Experimental; Argentina
dc.description.fil Fil: Pignataro, Omar Pedro. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biología y Medicina Experimental. Fundación de Instituto de Biología y Medicina Experimental. Instituto de Biología y Medicina Experimental; Argentina
dc.description.fil Fil: Stocco, Douglas M.. University of Texas; Estados Unidos
dc.journal.title Molecular and Cellular Endocrinology
dc.relation.alternativeid info:eu-repo/semantics/altIdentifier/url/https://www.sciencedirect.com/science/article/pii/S0303720701007481
dc.relation.alternativeid info:eu-repo/semantics/altIdentifier/doi/http://dx.doi.org/10.1016/S0303-7207(01)00748-1
dc.relation.alternativeid info:eu-repo/semantics/altIdentifier/pmid/11911946
dc.conicet.fuente unificacion


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info:eu-repo/semantics/openAccess Excepto donde se diga explícitamente, este item se publica bajo la siguiente descripción: Creative Commons Attribution-NonCommercial-ShareAlike 2.5 Unported (CC BY-NC-SA 2.5)