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dc.contributor.author
Wang, Xing Jia
dc.contributor.author
Dyson, Matthew T.
dc.contributor.author
Mondillo, Carolina
dc.contributor.author
Patrignani, Zoraida
dc.contributor.author
Pignataro, Omar Pedro
dc.contributor.author
Stocco, Douglas M.
dc.date.available
2018-02-08T21:09:04Z
dc.date.issued
2002-02-25
dc.identifier.citation
Wang, Xing Jia; Dyson, Matthew T.; Mondillo, Carolina; Patrignani, Zoraida; Pignataro, Omar Pedro; et al.; Interaction between arachidonic acid and cAMP signaling pathways enhance steroidogenesis and StAR gene expression in MA-10 Leydig tumor cells; Elsevier Ireland; Molecular and Cellular Endocrinology; 188; 1; 25-2-2002; 55-63
dc.identifier.issn
0303-7207
dc.identifier.uri
http://hdl.handle.net/11336/36241
dc.description.abstract
Previous studies have demonstrated that trophic hormone stimulation induced cyclic AMP (cAMP) formation and arachidonic acid (AA) release from phospholipids and that both these compounds were required for steroid biosynthesis and steroidogenic acute regulatory (StAR) gene expression in MA-10 mouse Leydig tumor cells. The present study further investigates the synergistic effects of the AA and cAMP interaction on steroidogenesis. To demonstrate cAMP-induced AA release, MA-10 cells were pre-loaded with 3H-AA and subsequently treated with dibutyryl cyclic AMP (dbcAMP). Stimulation with dbcAMP significantly induced AA release in MA-10 cells to a level 145.7% higher than that of controls. Lowering intracellular cAMP concentration by expressing a cAMP-phosphodiesterase significantly reduced human chorionic gonadotrophin (hCG)-induced AA release. The dbcAMP-induced AA release was inhibited significantly by the phospholipase A(2) (PLA(2)) inhibitor dexamethasone (Dex) and also by the protein kinase A (PKA) inhibitor H89, suggesting the involvement of PKA phosphorylation and/or PLA(2) activation in cAMP-induced AA release. The effect of the interaction between AA and cAMP on StAR gene expression and steroid production was also investigated. While 0.2 mM dbcAMP induced only very low levels of StAR protein, StAR mRNA, StAR promoter activity and steroid production, all of these parameters increased dramatically as AA concentration in the culture medium was increased from 0 to 200 microM. Importantly, AA was not able to induce a significant increase in steroidogenesis at any concentration when used in the absence of dbcAMP. However, when used in concert with submaximal concentrations of dbcAMP (0.05 mm to 0.5 mm), AA was capable of stimulating StAR gene expression and increasing steroid production significantly. The results from this study demonstrate that AA and cAMP act in a highly synergistic manner to increase the sensitivity of steroid production to trophic hormone stimulation and probably do so by increasing StAR gene expression.
dc.format
application/pdf
dc.language.iso
eng
dc.publisher
Elsevier Ireland
dc.rights
info:eu-repo/semantics/openAccess
dc.rights.uri
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.subject.classification
Bioquímica y Biología Molecular
dc.subject.classification
Ciencias Biológicas
dc.subject.classification
CIENCIAS NATURALES Y EXACTAS
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Patología
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Medicina Básica
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CIENCIAS MÉDICAS Y DE LA SALUD
dc.title
Interaction between arachidonic acid and cAMP signaling pathways enhance steroidogenesis and StAR gene expression in MA-10 Leydig tumor cells
dc.type
info:eu-repo/semantics/article
dc.type
info:ar-repo/semantics/artículo
dc.type
info:eu-repo/semantics/publishedVersion
dc.date.updated
2017-12-28T13:41:30Z
dc.identifier.eissn
1872-8057
dc.journal.volume
188
dc.journal.number
1
dc.journal.pagination
55-63
dc.journal.pais
Irlanda
dc.journal.ciudad
Limerick
dc.description.fil
Fil: Wang, Xing Jia. University of Texas; Estados Unidos
dc.description.fil
Fil: Dyson, Matthew T.. University of Texas; Estados Unidos
dc.description.fil
Fil: Mondillo, Carolina. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biología y Medicina Experimental. Fundación de Instituto de Biología y Medicina Experimental. Instituto de Biología y Medicina Experimental; Argentina
dc.description.fil
Fil: Patrignani, Zoraida. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biología y Medicina Experimental. Fundación de Instituto de Biología y Medicina Experimental. Instituto de Biología y Medicina Experimental; Argentina
dc.description.fil
Fil: Pignataro, Omar Pedro. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biología y Medicina Experimental. Fundación de Instituto de Biología y Medicina Experimental. Instituto de Biología y Medicina Experimental; Argentina
dc.description.fil
Fil: Stocco, Douglas M.. University of Texas; Estados Unidos
dc.journal.title
Molecular and Cellular Endocrinology
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/url/https://www.sciencedirect.com/science/article/pii/S0303720701007481
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/doi/http://dx.doi.org/10.1016/S0303-7207(01)00748-1
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/pmid/11911946


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