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dc.contributor.author
Weidinger, S.  
dc.contributor.author
Mayerhofer, Artur  
dc.contributor.author
Frungieri, Monica Beatriz  
dc.contributor.author
Meineke, V.  
dc.contributor.author
Ring, J.  
dc.contributor.author
Köhn, F. M.  
dc.date.available
2018-02-08T20:52:37Z  
dc.date.issued
2003  
dc.identifier.citation
Weidinger, S.; Mayerhofer, Artur; Frungieri, Monica Beatriz; Meineke, V.; Ring, J.; et al.; Mast cell-sperm interaction: evidence for tryptase and proteinase-activated receptors in the regulation of sperm motility; Oxford University Press; Human Reproduction; 18; 12; 2003; 2519-2524  
dc.identifier.issn
0268-1161  
dc.identifier.uri
http://hdl.handle.net/11336/36230  
dc.description.abstract
The detection of significant levels of tryptase in human seminal plasma and follicular fluid and of tryptase-positive mast cells (MCs) in the wall of human Fallopian tubes lead us to hypothesize that tryptase may exert regulatory actions on human spermatozoa. Immunoelectronmicroscopy revealed proteinase-activated receptor 2 (PAR-2) in the membranes of the acrosomal region and midpiece of human spermatozoa. These PAR-2 were functional, as exposure of spermatozoa from healthy men (n = 12) with regular standard semen parameters to human recombinant tryptase significantly decreased motility in a dose- and time-dependent fashion. Motile spermatozoa (WHO a + b) were significantly decreased within 10 min of incubation with 1.000 ng/ml tryptase (P = 0.045). After 30 and 60 min, significant reduction of motility was also observed in the presence of lower tryptase concentrations (100 ng/ml, P = 0.037; 10 ng/ml, P = 0.046). The inhibitory effects of tryptase progressed throughout an observation period of 180 min. Furthermore, tryptase effects were reversible after washing procedures and could be inhibited by pretreatment with anti-tryptase antibody or anti-PAR-2 antiserum.  
dc.format
application/pdf  
dc.language.iso
eng  
dc.publisher
Oxford University Press  
dc.rights
info:eu-repo/semantics/openAccess  
dc.rights.uri
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/  
dc.subject
Fertility  
dc.subject
Humano  
dc.subject
Sperm  
dc.subject
Tryptase  
dc.subject.classification
Inmunología  
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Medicina Básica  
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CIENCIAS MÉDICAS Y DE LA SALUD  
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Biología Reproductiva  
dc.subject.classification
Ciencias Biológicas  
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CIENCIAS NATURALES Y EXACTAS  
dc.title
Mast cell-sperm interaction: evidence for tryptase and proteinase-activated receptors in the regulation of sperm motility  
dc.type
info:eu-repo/semantics/article  
dc.type
info:ar-repo/semantics/artículo  
dc.type
info:eu-repo/semantics/publishedVersion  
dc.date.updated
2017-12-28T13:40:41Z  
dc.identifier.eissn
1460-2350  
dc.journal.volume
18  
dc.journal.number
12  
dc.journal.pagination
2519-2524  
dc.journal.pais
Reino Unido  
dc.journal.ciudad
Oxford  
dc.description.fil
Fil: Weidinger, S.. Technische Universität München; Alemania  
dc.description.fil
Fil: Mayerhofer, Artur. Universität München; Alemania  
dc.description.fil
Fil: Frungieri, Monica Beatriz. Universität München; Alemania. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biología y Medicina Experimental. Fundación de Instituto de Biología y Medicina Experimental. Instituto de Biología y Medicina Experimental; Argentina. Universidad de Buenos Aires. Facultad de Medicina; Argentina  
dc.description.fil
Fil: Meineke, V.. Military Academy Munich; Alemania  
dc.description.fil
Fil: Ring, J.. Technische Universität München; Alemania  
dc.description.fil
Fil: Köhn, F. M.. Technische Universität München; Alemania  
dc.journal.title
Human Reproduction  
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/url/https://academic.oup.com/humrep/article/18/12/2519/2913425  
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/doi/http://dx.doi.org/10.1093/humrep/deg476  
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/pmid/14645166