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dc.contributor.author
Omarini, Alejandra Beatriz
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dc.contributor.author
Plagemann, Ina
dc.contributor.author
Schimanski, Silke
dc.contributor.author
Krings, Ulrich
dc.contributor.author
Berger, Ralf G.
dc.date.available
2018-02-07T13:09:19Z
dc.date.issued
2014-08
dc.identifier.citation
Omarini, Alejandra Beatriz; Plagemann, Ina ; Schimanski, Silke; Krings, Ulrich ; Berger, Ralf G.; Crosses between monokaryons of Pleurotus sapidus or Pleurotus florida show an improved biotransformation of (+)-valencene to (+)-nootkatone; Elsevier; Bioresource Technology; 171; 8-2014; 113-119
dc.identifier.issn
0960-8524
dc.identifier.uri
http://hdl.handle.net/11336/35925
dc.description.abstract
Several hundred monokaryotic and new dikaryotic strains derived thereof were established from (+)-valencene tolerant Pleurotus species. When grouped according to their growth rate on agar plates and compared to the parental of Pleurotus sapidus 69, the slowly growing monokaryons converted (+)-valencene more efficiently to the grapefruit flavour compound (+)-nootkatone. The fast growing monokaryons and the slow × slow and the fast × fast dikaryotic crosses showed similar or inferior yields. Some slow × fast dikaryons, however, exceeded the biotransformation capability of the parental dikaryon significantly. The activity of the responsible enzyme, lipoxygenase, showed a weak correlation with the yields of (+)-nootkatone indicating that the determination of enzyme activity using the primary substrate linoleic acid may be misleading in predicting the biotransformation efficiency. This exploratory study indicated that a classical genetics approach resulted in altered and partly improved terpene transformation capability (plus 60%) and lipoxygenase activity of the strains.
dc.format
application/pdf
dc.language.iso
eng
dc.publisher
Elsevier
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dc.rights
info:eu-repo/semantics/openAccess
dc.rights.uri
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.subject
Pleurotus
dc.subject
Basidiomycota
dc.subject
Monokaryon
dc.subject
(+)-Valencene
dc.subject
Biotransformation
dc.subject.classification
Otras Ciencias Biológicas
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dc.subject.classification
Ciencias Biológicas
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dc.subject.classification
CIENCIAS NATURALES Y EXACTAS
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dc.title
Crosses between monokaryons of Pleurotus sapidus or Pleurotus florida show an improved biotransformation of (+)-valencene to (+)-nootkatone
dc.type
info:eu-repo/semantics/article
dc.type
info:ar-repo/semantics/artículo
dc.type
info:eu-repo/semantics/publishedVersion
dc.date.updated
2018-02-06T20:06:29Z
dc.journal.volume
171
dc.journal.pagination
113-119
dc.journal.pais
Países Bajos
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dc.journal.ciudad
Amsterdam
dc.description.fil
Fil: Omarini, Alejandra Beatriz. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Leibniz Universität Hannover. Hannover; Alemania
dc.description.fil
Fil: Plagemann, Ina. Leibniz Universität Hannover. Hannover; Alemania
dc.description.fil
Fil: Schimanski, Silke. Leibniz Universität Hannover. Hannover; Alemania
dc.description.fil
Fil: Krings, Ulrich. Leibniz Universität Hannover. Hannover; Alemania
dc.description.fil
Fil: Berger, Ralf G.. Leibniz Universität Hannover. Hannover; Alemania
dc.journal.title
Bioresource Technology
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dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/doi/http://dx.doi.org/10.1016/j.biortech.2014.08.061
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/url/https://www.sciencedirect.com/science/article/pii/S0960852414011778
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