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dc.contributor.author
Gupta, Alok K.
dc.contributor.author
Abatedaga, Maria Ines de Los Angeles
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Pichersky, Eran
dc.contributor.author
Schiestl, Florian P.
dc.date.available
2018-02-06T20:37:09Z
dc.date.issued
2014-09
dc.identifier.citation
Gupta, Alok K.; Abatedaga, Maria Ines de Los Angeles; Pichersky, Eran; Schiestl, Florian P.; Eugenol synthase genes in floral scent variation in Gymnadenia species; Springer Heidelberg; Functional & Integrative Genomics; 14; 4; 9-2014; 779-788
dc.identifier.issn
1438-793X
dc.identifier.uri
http://hdl.handle.net/11336/35887
dc.description.abstract
Floral signaling, especially through floral scent, is often highly complex, and little is known about the molecular mechanisms and evolutionary causes of this complexity. In this study, we focused on the evolution of “floral scent genes” and the associated changes in their functions in three closely related orchid species of the genus Gymnadenia. We developed a benchmark repertoire of 2,571 expressed sequence tags (ESTs) in Gymnadenia odoratissima. For the functional characterization and evolutionary analysis, we focused on eugenol synthase, as eugenol is a widespread and important scent compound. We obtained complete coding complementary DNAs (cDNAs) of two copies of putative eugenol synthase genes in each of the three species. The proteins encoded by these cDNAs were characterized by expression and testing for activity in Escherichia coli. While G. odoratissima and Gymnadenia conopsea enzymes were found to catalyze the formation of eugenol only, the Gymnadenia densiflora proteins synthesize eugenol, as well as a smaller amount of isoeugenol. Finally, we showed that the eugenol and isoeugenol producing gene copies of G. densiflora are evolutionarily derived from the ancestral genes of the other species producing only eugenol. The evolutionary switch from production of one to two compounds evolved under relaxed purifying selection. In conclusion, our study shows the molecular bases of eugenol and isoeugenol production and suggests that an evolutionary transition in a single gene can lead to an increased complexity in floral scent emitted by plants.
dc.format
application/pdf
dc.language.iso
eng
dc.publisher
Springer Heidelberg
dc.rights
info:eu-repo/semantics/openAccess
dc.rights.uri
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.subject
Ests
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Eugenol
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Floral Scents
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Gymnadenia
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Pollination
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Isoeugenol
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Voc
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Otras Ciencias Biológicas
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Ciencias Biológicas
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CIENCIAS NATURALES Y EXACTAS
dc.title
Eugenol synthase genes in floral scent variation in Gymnadenia species
dc.type
info:eu-repo/semantics/article
dc.type
info:ar-repo/semantics/artículo
dc.type
info:eu-repo/semantics/publishedVersion
dc.date.updated
2018-02-06T20:05:49Z
dc.journal.volume
14
dc.journal.number
4
dc.journal.pagination
779-788
dc.journal.pais
Alemania
dc.journal.ciudad
Berlin
dc.description.fil
Fil: Gupta, Alok K.. Universitat Zurich; Suiza. Plant Ecological Genetics. Zurich; Suiza
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Fil: Abatedaga, Maria Ines de Los Angeles. University of Michigan; Estados Unidos
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Fil: Pichersky, Eran. University of Michigan; Estados Unidos. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro de Investigaciones y Transferencia de Santiago del Estero. Universidad Nacional de Santiago del Estero. Centro de Investigaciones y Transferencia de Santiago del Estero; Argentina
dc.description.fil
Fil: Schiestl, Florian P.. Universitat Zurich; Suiza
dc.journal.title
Functional & Integrative Genomics
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/doi/http://dx.doi.org/10.1007/s10142-014-0397-9
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/url/https://link.springer.com/article/10.1007%2Fs10142-014-0397-9
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