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dc.contributor.author
Uyua, Noelia Mariel  
dc.contributor.author
Manrique, Julieta Marina  
dc.contributor.author
Jones, Leandro Roberto  
dc.date.available
2018-02-05T20:53:59Z  
dc.date.issued
2014-06  
dc.identifier.citation
Uyua, Noelia Mariel; Manrique, Julieta Marina; Jones, Leandro Roberto; An optimized DNA extraction protocol for benthic Didymosphenia geminata; Elsevier Science; Journal Of Microbiological Methods; 104; 6-2014; 12-18  
dc.identifier.issn
0167-7012  
dc.identifier.uri
http://hdl.handle.net/11336/35693  
dc.description.abstract
Didymosphenia geminata mats display few cells in relation to extracellular material and contain polysaccharides and heavy metals that interfere with molecular studies. We describe an optimized DNA extraction protocol that help to overcome these difficulties. Our protocol outperformed five previously described DNA extraction techniques.  
dc.format
application/pdf  
dc.language.iso
eng  
dc.publisher
Elsevier Science  
dc.rights
info:eu-repo/semantics/openAccess  
dc.rights.uri
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/  
dc.subject
Didymosphenia  
dc.subject
Dna Extraction  
dc.subject
Pcr  
dc.subject
Stalk  
dc.subject
Benthic  
dc.subject
Invasive  
dc.subject.classification
Otras Ciencias Biológicas  
dc.subject.classification
Ciencias Biológicas  
dc.subject.classification
CIENCIAS NATURALES Y EXACTAS  
dc.title
An optimized DNA extraction protocol for benthic Didymosphenia geminata  
dc.type
info:eu-repo/semantics/article  
dc.type
info:ar-repo/semantics/artículo  
dc.type
info:eu-repo/semantics/publishedVersion  
dc.date.updated
2018-02-05T20:22:33Z  
dc.journal.volume
104  
dc.journal.pagination
12-18  
dc.journal.pais
Países Bajos  
dc.journal.ciudad
Amsterdam  
dc.description.fil
Fil: Uyua, Noelia Mariel. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional de la Patagonia "San Juan Bosco". Facultad de Ciencias Naturales - Sede Trelew; Argentina  
dc.description.fil
Fil: Manrique, Julieta Marina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional de la Patagonia "San Juan Bosco". Facultad de Ciencias Naturales - Sede Trelew; Argentina  
dc.description.fil
Fil: Jones, Leandro Roberto. Universidad Nacional de la Patagonia "San Juan Bosco". Facultad de Ciencias Naturales - Sede Trelew; Argentina  
dc.journal.title
Journal Of Microbiological Methods  
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/doi/http://dx.doi.org/10.1016/j.mimet.2014.06.007  
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/url/https://www.sciencedirect.com/science/article/pii/S0167701214001626