Artículo
Alcohol induction of liver nuclear ethanol and N-nitrosodimethylamine metabolism to reactive metabolites
Diaz Gomez, Maria Isabel
; Valles, E.; Fanelli, Silvia Laura
; Delgado, Aurora Maria
; Castro, Gerardo Daniel
; Castro, Jose Alberto
Fecha de publicación:
01/2002
Editorial:
Wiley
Revista:
Teratogenesis Carcinogenesis And Mutagenesis
ISSN:
0270-3211
Idioma:
Inglés
Tipo de recurso:
Artículo publicado
Clasificación temática:
Resumen
In previous studies from our laboratory we reported the presence in highly purified liver nuclei, free of contamination with other organelles, of an ethanol metabolizing system (NEMS) able to lead to acetaldehyde and 1-hydroxyethyl free radicals (1HEt). In the present study we tested whether this NEMS is inducible by chronic alcohol administration to rats and whether these nuclei also have increased ability to bioactivate N-nitrosodimethylamine (NDMA). Sprague Dawley male rats (125-150g) were fed with a nutritionally adequate liquid diet containing alcohol to provide 36% of total energy (standard Lieber-De Carli rat diet), for 28 days. Controls received an isocaloric diet without alcohol. Animals were sacrificed, livers were excised and microsomes and purified nuclear fractions were prepared. Both microsomes and nuclei from treated animals had significantly increased ability compared to controls, to biotransform ethanol to acetaldehyde using NADPH as cofactor under an air atmosphere. Both organelles also exhibited significantly increased capacity compared to controls, to bioactivate NDMA to formaldehyde and to reactive metabolites that bind covalently to proteins. Nuclear preparations from control animals were also able to metabolize NDMA to formaldehyde and reactive metabolites. Results indicate that liver nuclei may have a CYP2E1 able to bioactivate both NDMA and EtOH and that these processes are being induced by chronic alcohol drinking. The bioactivation of these xenobiotics to reactive metabolites in the neighborhood of nuclear proteins and DNA might have significant toxicological implications.
Palabras clave:
Ethanol
,
1-Hydroxyethyl Free Radicals
,
N-Nitrosodimethylamine
,
Liver Nuclei
Archivos asociados
Licencia
Identificadores
Colecciones
Articulos(ILPLA)
Articulos de INST.DE LIMNOLOGIA "DR. RAUL A. RINGUELET"
Articulos de INST.DE LIMNOLOGIA "DR. RAUL A. RINGUELET"
Articulos(SEDE CENTRAL)
Articulos de SEDE CENTRAL
Articulos de SEDE CENTRAL
Articulos(UNIDEF)
Articulos de UNIDAD DE INVESTIGACION Y DESARROLLO ESTRATEGICOS PARA LA DEFENSA
Articulos de UNIDAD DE INVESTIGACION Y DESARROLLO ESTRATEGICOS PARA LA DEFENSA
Citación
Diaz Gomez, Maria Isabel; Valles, E.; Fanelli, Silvia Laura; Delgado, Aurora Maria; Castro, Gerardo Daniel; et al.; Alcohol induction of liver nuclear ethanol and N-nitrosodimethylamine metabolism to reactive metabolites; Wiley; Teratogenesis Carcinogenesis And Mutagenesis; 22; 2; 1-2002; 139-145
Compartir
Altmétricas