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dc.contributor.author
Furuse, Yuki
dc.contributor.author
Finethy, Ryan
dc.contributor.author
Saka, Hector Alex
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dc.contributor.author
Xet Mull, Ana M.
dc.contributor.author
Sisk, Dana M.
dc.contributor.author
Jurcic Smith, Kristen L.
dc.contributor.author
Lee, Sunhee
dc.contributor.author
Coers, Jörn
dc.contributor.author
Valdivia, Raphael H.
dc.contributor.author
Tobin, David M.
dc.contributor.author
Cullen, Bryan R.
dc.date.available
2018-01-30T18:13:48Z
dc.date.issued
2014-09
dc.identifier.citation
Furuse, Yuki; Finethy, Ryan; Saka, Hector Alex; Xet Mull, Ana M.; Sisk, Dana M.; et al.; Search for MicroRNAs Expressed by Intracellular Bacterial Pathogens in Infected Mammalian Cells; Public Library of Science; Plos One; 9; 9; 9-2014; 1-13; e106434
dc.identifier.issn
1932-6203
dc.identifier.uri
http://hdl.handle.net/11336/35043
dc.description.abstract
MicroRNAs are expressed by all multicellular organisms and play a critical role as post-transcriptional regulators of gene expression. Moreover, different microRNA species are known to influence the progression of a range of different diseases, including cancer and microbial infections. A number of different human viruses also encode microRNAs that can attenuate cellular innate immune responses and promote viral replication, and a fungal pathogen that infects plants has recently been shown to express microRNAs in infected cells that repress host cell immune responses and promote fungal pathogenesis. Here, we have used deep sequencing of total expressed small RNAs, as well as small RNAs associated with the cellular RNA-induced silencing complex RISC, to search for microRNAs that are potentially expressed by intracellular bacterial pathogens and translocated into infected animal cells. In the case of Legionella and Chlamydia and the two mycobacterial species M. smegmatis and M. tuberculosis, we failed to detect any bacterial small RNAs that had the characteristics expected for authentic microRNAs, although large numbers of small RNAs of bacterial origin could be recovered. However, a third mycobacterial species, M. marinum, did express an ∼23-nt small RNA that was bound by RISC and derived from an RNA stem-loop with the characteristics expected for a pre-microRNA. While intracellular expression of this candidate bacterial microRNA was too low to effectively repress target mRNA species in infected cultured cells in vitro, artificial overexpression of this potential bacterial pre-microRNA did result in the efficient repression of a target mRNA. This bacterial small RNA therefore represents the first candidate microRNA of bacterial origin.
dc.format
application/pdf
dc.language.iso
eng
dc.publisher
Public Library of Science
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dc.rights
info:eu-repo/semantics/openAccess
dc.rights.uri
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.subject
Micrornas
dc.subject
Intracellular
dc.subject
Bacteria
dc.subject
Pathogens
dc.subject.classification
Otras Ciencias Biológicas
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dc.subject.classification
Ciencias Biológicas
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dc.subject.classification
CIENCIAS NATURALES Y EXACTAS
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dc.title
Search for MicroRNAs Expressed by Intracellular Bacterial Pathogens in Infected Mammalian Cells
dc.type
info:eu-repo/semantics/article
dc.type
info:ar-repo/semantics/artículo
dc.type
info:eu-repo/semantics/publishedVersion
dc.date.updated
2018-01-29T19:44:55Z
dc.journal.volume
9
dc.journal.number
9
dc.journal.pagination
1-13; e106434
dc.journal.pais
Estados Unidos
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dc.journal.ciudad
San Francisco
dc.description.fil
Fil: Furuse, Yuki. University of Duke; Estados Unidos
dc.description.fil
Fil: Finethy, Ryan. University of Duke; Estados Unidos
dc.description.fil
Fil: Saka, Hector Alex. University of Duke; Estados Unidos. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
dc.description.fil
Fil: Xet Mull, Ana M.. University of Duke; Estados Unidos
dc.description.fil
Fil: Sisk, Dana M.. University of Duke; Estados Unidos
dc.description.fil
Fil: Jurcic Smith, Kristen L.. University of Duke; Estados Unidos
dc.description.fil
Fil: Lee, Sunhee. University of Duke; Estados Unidos
dc.description.fil
Fil: Coers, Jörn. University of Duke; Estados Unidos
dc.description.fil
Fil: Valdivia, Raphael H.. University of Duke; Estados Unidos
dc.description.fil
Fil: Tobin, David M.. University of Duke; Estados Unidos
dc.description.fil
Fil: Cullen, Bryan R.. University of Duke; Estados Unidos
dc.journal.title
Plos One
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dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/url/https://www.ncbi.nlm.nih.gov/pmc/articles/pmid/25184567/
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/doi/http://dx.doi.org/10.1371/journal.pone.0106434
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/url/http://journals.plos.org/plosone/article?id=10.1371/journal.pone.0106434
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