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dc.contributor.author
Campos, F. S.
dc.contributor.author
Franco, A. C.
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Oliveira, M. T.
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Firpo, R.
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Strelczuk, G.
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Fontoura, F. E.
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Kulmann, M. I. R.
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Maidana, Silvina Soledad
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Romera, Sonia
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Spilki, F. R.
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Silva, A. D.
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Hübner, S. O.
dc.contributor.author
Roehe, P. M.
dc.date.available
2018-01-29T18:30:10Z
dc.date.issued
2014-03
dc.identifier.citation
Campos, F. S.; Franco, A. C.; Oliveira, M. T.; Firpo, R.; Strelczuk, G.; et al.; Detection of bovine herpesvirus 2 and bovine herpesvirus 4 DNA in trigeminal ganglia of naturally infected cattle by polymerase chain reaction; Elsevier; Veterin Microbiology; 171; 1-2; 3-2014; 182-188
dc.identifier.issn
1873-2542
dc.identifier.uri
http://hdl.handle.net/11336/34919
dc.description.abstract
Establishment of latent infection within specific tissues in the host is a common biological feature of the herpesviruses. In the case of bovine herpesvirus 2 (BoHV-2), latency is established in neuronal tissues, while bovine herpesvirus 4 (BoHV-4) and ovine herpesvirus 2 (OvHV-2) latent virus targets on cells of the monocytic lineage. This study was conducted in quest of BoHV-2, BoHV-4 and OvHV-2 DNA in two hundred trigeminal ganglia (TG) specimens, derived from one hundred clinically healthy cattle, majority of them naturally infected with bovine herpesvirus 1 (BoHV-1) and bovine herpesvirus 5 (BoHV-5). Total DNA extracted from ganglia was analyzed by polymerase chain reaction (PCR) designed to amplify part of the genes coding for BoHV-2, and BoHV-4 glycoprotein B and, for OvHV-2, the gene coding for phosphoribosylformylglycinamidine synthase-like protein. BoHV-2 DNA was detected in TG samples of two (2%) and BoHV-4 DNA in nine (9%) of the animals, whereas OvHV-2 DNA could not be detected in any of the TG DNA. The two animals in which BoHV-2 DNA was identified were also co-infected with BoHV-1 and BoHV-5. Within the nine animals in which BoHV-4 DNA was detected, six were also co-infected with BoHV-1 and BoHV-5. This report provides for the first time evidence that viral DNA from BoHV-2 and BoHV-4 can be occasionally detected in TG of naturally infected cattle. Likewise, in this report we provided for the first time evidence that the co-infection of cattle with three distinct bovine herpesviruses might be a naturally occurring phenomenon.
dc.format
application/pdf
dc.language.iso
eng
dc.publisher
Elsevier
dc.rights
info:eu-repo/semantics/openAccess
dc.rights.uri
https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
dc.subject
Bovine Herpesvirus
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Ovine Herpesvirus
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Pcr
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Latency
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Internal Control
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Otras Ciencias Biológicas
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Ciencias Biológicas
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CIENCIAS NATURALES Y EXACTAS
dc.title
Detection of bovine herpesvirus 2 and bovine herpesvirus 4 DNA in trigeminal ganglia of naturally infected cattle by polymerase chain reaction
dc.type
info:eu-repo/semantics/article
dc.type
info:ar-repo/semantics/artículo
dc.type
info:eu-repo/semantics/publishedVersion
dc.date.updated
2018-01-29T14:04:26Z
dc.journal.volume
171
dc.journal.number
1-2
dc.journal.pagination
182-188
dc.journal.pais
Países Bajos
dc.journal.ciudad
Ámsterdam
dc.description.fil
Fil: Campos, F. S.. Universidade Federal do Rio Grande do Sul; Brasil
dc.description.fil
Fil: Franco, A. C.. Universidade Federal do Rio Grande do Sul; Brasil
dc.description.fil
Fil: Oliveira, M. T.. Universidade Federal do Rio Grande do Sul; Brasil
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Fil: Firpo, R.. Universidade Federal do Rio Grande do Sul; Brasil
dc.description.fil
Fil: Strelczuk, G.. Universidade Federal do Rio Grande do Sul; Brasil
dc.description.fil
Fil: Fontoura, F. E.. Universidade Federal do Rio Grande do Sul; Brasil
dc.description.fil
Fil: Kulmann, M. I. R.. Universidade Federal do Rio Grande do Sul; Brasil
dc.description.fil
Fil: Maidana, Silvina Soledad. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigación en Ciencias Veterinarias y Agronómicas. Instituto de Virología; Argentina
dc.description.fil
Fil: Romera, Sonia. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigación en Ciencias Veterinarias y Agronómicas. Instituto de Virología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
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Fil: Spilki, F. R.. Universidade Feevale; Brasil
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Fil: Silva, A. D.. Ministerio da Agricultura Pecuaria e Abastecimento de Brasil. Empresa Brasileira de Pesquisa Agropecuaria; Brasil
dc.description.fil
Fil: Hübner, S. O.. Universidade Federal de Pelotas; Brasil
dc.description.fil
Fil: Roehe, P. M.. Universidade Federal do Rio Grande do Sul; Brasil. Instituto de Pesquisa Veterinária Desiderio Finamor; Brasil
dc.journal.title
Veterin Microbiology


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