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dc.contributor.author Campos, F. S.
dc.contributor.author Franco, A. C.
dc.contributor.author Oliveira, M. T.
dc.contributor.author Firpo, R.
dc.contributor.author Strelczuk, G.
dc.contributor.author Fontoura, F. E.
dc.contributor.author Kulmann, M. I. R.
dc.contributor.author Maidana, Silvina Soledad
dc.contributor.author Romera, Sonia
dc.contributor.author Spilki, F. R.
dc.contributor.author Silva, A. D.
dc.contributor.author Hübner, S. O.
dc.contributor.author Roehe, P. M.
dc.date.available 2018-01-29T18:30:10Z
dc.date.issued 2014-03
dc.identifier.citation Campos, F. S.; Franco, A. C.; Oliveira, M. T.; Firpo, R.; Strelczuk, G.; et al.; Detection of bovine herpesvirus 2 and bovine herpesvirus 4 DNA in trigeminal ganglia of naturally infected cattle by polymerase chain reaction; Elsevier; Veterin Microbiology; 171; 1-2; 3-2014; 182-188
dc.identifier.issn 1873-2542
dc.identifier.uri http://hdl.handle.net/11336/34919
dc.description.abstract Establishment of latent infection within specific tissues in the host is a common biological feature of the herpesviruses. In the case of bovine herpesvirus 2 (BoHV-2), latency is established in neuronal tissues, while bovine herpesvirus 4 (BoHV-4) and ovine herpesvirus 2 (OvHV-2) latent virus targets on cells of the monocytic lineage. This study was conducted in quest of BoHV-2, BoHV-4 and OvHV-2 DNA in two hundred trigeminal ganglia (TG) specimens, derived from one hundred clinically healthy cattle, majority of them naturally infected with bovine herpesvirus 1 (BoHV-1) and bovine herpesvirus 5 (BoHV-5). Total DNA extracted from ganglia was analyzed by polymerase chain reaction (PCR) designed to amplify part of the genes coding for BoHV-2, and BoHV-4 glycoprotein B and, for OvHV-2, the gene coding for phosphoribosylformylglycinamidine synthase-like protein. BoHV-2 DNA was detected in TG samples of two (2%) and BoHV-4 DNA in nine (9%) of the animals, whereas OvHV-2 DNA could not be detected in any of the TG DNA. The two animals in which BoHV-2 DNA was identified were also co-infected with BoHV-1 and BoHV-5. Within the nine animals in which BoHV-4 DNA was detected, six were also co-infected with BoHV-1 and BoHV-5. This report provides for the first time evidence that viral DNA from BoHV-2 and BoHV-4 can be occasionally detected in TG of naturally infected cattle. Likewise, in this report we provided for the first time evidence that the co-infection of cattle with three distinct bovine herpesviruses might be a naturally occurring phenomenon.
dc.format application/pdf
dc.language.iso eng
dc.publisher Elsevier
dc.rights info:eu-repo/semantics/openAccess
dc.rights.uri https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
dc.subject Bovine herpesvirus
dc.subject Ovine herpesvirus
dc.subject PCR
dc.subject Latency
dc.subject Internal control
dc.subject.classification Otras Ciencias Biológicas
dc.subject.classification Ciencias Biológicas
dc.subject.classification CIENCIAS NATURALES Y EXACTAS
dc.title Detection of bovine herpesvirus 2 and bovine herpesvirus 4 DNA in trigeminal ganglia of naturally infected cattle by polymerase chain reaction
dc.type info:eu-repo/semantics/article
dc.type info:ar-repo/semantics/artículo
dc.type info:eu-repo/semantics/publishedVersion
dc.date.updated 2018-01-29T14:04:26Z
dc.journal.volume 171
dc.journal.number 1-2
dc.journal.pagination 182-188
dc.journal.pais Países Bajos
dc.journal.ciudad Ámsterdam
dc.description.fil Fil: Campos, F. S.. Universidade Federal do Rio Grande do Sul; Brasil
dc.description.fil Fil: Franco, A. C.. Universidade Federal do Rio Grande do Sul; Brasil
dc.description.fil Fil: Oliveira, M. T.. Universidade Federal do Rio Grande do Sul; Brasil
dc.description.fil Fil: Firpo, R.. Universidade Federal do Rio Grande do Sul; Brasil
dc.description.fil Fil: Strelczuk, G.. Universidade Federal do Rio Grande do Sul; Brasil
dc.description.fil Fil: Fontoura, F. E.. Universidade Federal do Rio Grande do Sul; Brasil
dc.description.fil Fil: Kulmann, M. I. R.. Universidade Federal do Rio Grande do Sul; Brasil
dc.description.fil Fil: Maidana, Silvina Soledad. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigación en Ciencias Veterinarias y Agronómicas. Instituto de Virología; Argentina
dc.description.fil Fil: Romera, Sonia. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigación en Ciencias Veterinarias y Agronómicas. Instituto de Virología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
dc.description.fil Fil: Spilki, F. R.. Universidade Feevale; Brasil
dc.description.fil Fil: Silva, A. D.. Ministerio da Agricultura Pecuaria e Abastecimento de Brasil. Empresa Brasileira de Pesquisa Agropecuaria; Brasil
dc.description.fil Fil: Hübner, S. O.. Universidade Federal de Pelotas; Brasil
dc.description.fil Fil: Roehe, P. M.. Universidade Federal do Rio Grande do Sul; Brasil. Instituto de Pesquisa Veterinária Desiderio Finamor; Brasil
dc.journal.title Veterin Microbiology
dc.conicet.fuente unificacion


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info:eu-repo/semantics/openAccess Excepto donde se diga explícitamente, este item se publica bajo la siguiente descripción: Atribución-NoComercial-SinDerivadas 2.5 Argentina (CC BY-NC-ND 2.5 AR)