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dc.contributor.author
Qüesta, Julia I.
dc.contributor.author
Walbot, Virginia
dc.contributor.author
Casati, Paula
dc.date.available
2016-01-07T20:01:43Z
dc.date.issued
2013-11
dc.identifier.citation
Qüesta, Julia I.; Walbot, Virginia; Casati, Paula; UV-B radiation induces Mu element somatic transposition in maize; Oxford University Press; Molecular Plant; 6; 11-2013; 2004-2007
dc.identifier.issn
1674-2052
dc.identifier.uri
http://hdl.handle.net/11336/3426
dc.description.abstract
The maize Mutator (MuDR/Mu) transposon family is the most active DNA transposon inplants (Lisch 2002). The Mu family contains diverse elements, all sharing similar ~215 bpterminal inverted repeats (TIRs). MuDR is the autonomous element, and a transcriptionallyactive MuDR is required for transposition of the non-autonomous Mu elements (Chomet et al.,1991). MuDR contains two genes, mudrA and mudrB (Figure 1A); mudrA encodes the MURAtransposase, and mudrB encodes a protein with unknown function. To avoid the deleterious effects of transposons, plants have acquired mechanisms to epigenetically silence them. Silenced Mu elements become heavily methylated, and their reactivation is very rare. To date, only UV-B radiation treatments have reactivated silenced Mu (Walbot 1999). We previously demonstrated that UV-B radiation modifies the chromatin at MuDR/Mu loci, triggering the expression of the transposase (Questa et al., 2010). MURA transposase interacts in vitro with a 32 bp domain, highly conserved in all mobile Mu TIRs (Benito and Walbot 1997). Furthermore, MURA can bind unmethylated, methylated and hemimethylated forms of its target sequence, therefore, the production of MURA is the rate-limiting step in reactivation of silent Mu elements. In the present work, we aim to elucidate the in vivo binding of MURA to its target sequence, and whether this interaction is affected by UV-B radiation.
dc.format
application/pdf
dc.language.iso
eng
dc.publisher
Oxford University Press
dc.rights
info:eu-repo/semantics/openAccess
dc.rights.uri
https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
dc.subject
Mutator
dc.subject
Transposon
dc.subject
Uv-B
dc.subject
Maiz
dc.subject.classification
Bioquímica y Biología Molecular
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Ciencias Biológicas
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CIENCIAS NATURALES Y EXACTAS
dc.title
UV-B radiation induces Mu element somatic transposition in maize
dc.type
info:eu-repo/semantics/article
dc.type
info:ar-repo/semantics/artículo
dc.type
info:eu-repo/semantics/publishedVersion
dc.date.updated
2016-03-30 10:35:44.97925-03
dc.journal.volume
6
dc.journal.pagination
2004-2007
dc.journal.pais
Reino Unido
dc.journal.ciudad
Oxford
dc.description.fil
Fil: Qüesta, Julia I.. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Rosario. Centro de Estudios Fotosintéticos y Bioquímicos (i); Argentina
dc.description.fil
Fil: Walbot, Virginia. University Of Stanford; Estados Unidos
dc.description.fil
Fil: Casati, Paula. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Rosario. Centro de Estudios Fotosintéticos y Bioquímicos (i); Argentina
dc.journal.title
Molecular Plant
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/doi/10.1093/mp/sst112
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/url/http://www.sciencedirect.com/science/article/pii/S1674205214602835
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/doi/http://dx.doi.org/10.1093/mp/sst112
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