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dc.contributor.author
Liffourrena, Andres Sebastian
dc.contributor.author
Lucchesi, Gloria Ines
dc.date.available
2018-01-19T14:30:57Z
dc.date.issued
2014-03
dc.identifier.citation
Liffourrena, Andres Sebastian; Lucchesi, Gloria Ines; Degradation of trimethylamine by immobilized cells of Pseudomonas putida A (ATCC 12633); Elsevier; International Biodeterioration and Biodegradation; 90; 3-2014; 88-92
dc.identifier.issn
0964-8305
dc.identifier.uri
http://hdl.handle.net/11336/33938
dc.description.abstract
Pseudomonas putida A (ATCC 12633) capable of degrading trimethylamine (TMA) was immobilized in calcium alginate. The TMA-degrading capacity of immobilized cells was compared to free cells in batch culture by changing temperature, pH, nutrient supplementation, and initial TMA concentration. Although immobilized cells showed good removal efficiencies in wider ranges of temperature (15 °C–35 °C) and pH (6.5–8.5) than free cells, the optimal conditions for TMA removal for both free and immobilized cells were 30 °C, in buffered medium (pH 7.5) without addition of nutrients. Immobilized cells degraded up 885 mg l−1 of TMA completely whereas the free cells degraded only 295 mg l−1. For all the TMA concentrations evaluated with immobilized cells (295–885 mg l−1), after 24 h, the degradation rate started to decline. This effect was attributed to the accumulation of intracellular TMA into the cells (8.3–15.3 mg l−1) which is sufficient to inhibit the first enzyme of the aerobic degradation of TMA by P. putida, the TMA dehydrogenase. The fact that the immobilized cells showed a high stability as regards their viability and degradability of high TMA concentrations revealed a good potential of this system for treating of TMA-contaminated site.
dc.format
application/pdf
dc.language.iso
eng
dc.publisher
Elsevier
dc.rights
info:eu-repo/semantics/restrictedAccess
dc.rights.uri
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.subject
Trimethylamine
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Pseudomonas Putida
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Biodegradation
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Alginate
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Otras Ciencias Biológicas
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Ciencias Biológicas
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CIENCIAS NATURALES Y EXACTAS
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Bioremediación, Diagnóstico Biotecnológico en Gestión Medioambiental
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Biotecnología del Medio Ambiente
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INGENIERÍAS Y TECNOLOGÍAS
dc.title
Degradation of trimethylamine by immobilized cells of Pseudomonas putida A (ATCC 12633)
dc.type
info:eu-repo/semantics/article
dc.type
info:ar-repo/semantics/artículo
dc.type
info:eu-repo/semantics/publishedVersion
dc.date.updated
2018-01-18T21:02:07Z
dc.journal.volume
90
dc.journal.pagination
88-92
dc.journal.pais
Países Bajos
dc.journal.ciudad
Amsterdam
dc.description.fil
Fil: Liffourrena, Andres Sebastian. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas Fisicoquímicas y Naturales. Departamento de Biología Molecular; Argentina
dc.description.fil
Fil: Lucchesi, Gloria Ines. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas Fisicoquímicas y Naturales. Departamento de Biología Molecular; Argentina
dc.journal.title
International Biodeterioration and Biodegradation
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/doi/http://dx.doi.org/10.1016/j.ibiod.2014.02.008
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/url/http://www.sciencedirect.com/science/article/pii/S0964830514000535