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dc.contributor.author
Goldschmidt, Ezequiel Darío
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dc.contributor.author
Ielpi, Marcelo
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dc.contributor.author
Loresi, Monica Alejandra
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dc.contributor.author
D'adamo, Maximiliano
dc.contributor.author
Giunta, Diego Hernan
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dc.contributor.author
Carrizo, Antonio
dc.contributor.author
Ajler, Pablo
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dc.contributor.author
Yampolsky, Claudio
dc.contributor.author
Argibay, Pablo
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dc.date.available
2018-01-18T21:01:09Z
dc.date.issued
2014-04
dc.identifier.citation
Goldschmidt, Ezequiel Darío; Ielpi, Marcelo; Loresi, Monica Alejandra; D'adamo, Maximiliano; Giunta, Diego Hernan; et al.; Assessing the role of selected growth factors and cytostatic agents in an in vitro model of human dura mater healing; Taylor & Francis; Neurological Research; 36; 12; 4-2014; 1040-1046
dc.identifier.issn
0161-6412
dc.identifier.uri
http://hdl.handle.net/11336/33896
dc.description.abstract
OBJECTIVES: Cerebrospinal fluid (CSF) leaks are a common concern in skull base surgery. Appropriate dural healing is crucial to prevent CSF leaks but the entire process has been barely understood so far. Here, we review the impact of growth factors and chemotherapeutic agents on an explant culture of human dural fibroblasts and a 3D subculture grown in a collagen mesh scaffold.
METHODS: Human dural specimens were harvested during surgical procedures where they would not be further used therapeutically or diagnostically. Explant cultures were grown in Petri dishes, and subcultures were grown in collagen mesh scaffolds. Insulin, fibroblast growth factor type 2 (FGF-2), and human serum were analyzed for their effect as growth factors, whereas mitomycin C, vincristine, and colchicine were analyzed for their role as inhibitors. Cell count was used as a parameter to assess the effects of these factors. In addition, the effects of human serum were assessed using collagen mesh scaffolds.
RESULTS: Insulin, FGF-2, and human serum increased culture cell count; human serum also achieved an increased number of viable fibroblasts embedded in a collagen mesh. Mitomycin C, which is a mitosis inhibitor, showed no significant effect on cell count, whereas colchicine and vincristine, which inhibit both mitosis and migration, resulted in cell growth suppression.
DISCUSSION: In our model, dural defect closure is achieved through cell migration rather than through cell growth. Adding growth factors to the dural suture line or into a collagen mesh might prove useful to stimulate dural closure.
dc.format
application/pdf
dc.language.iso
eng
dc.publisher
Taylor & Francis
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dc.rights
info:eu-repo/semantics/openAccess
dc.rights.uri
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.subject
Dura Mater
dc.subject
Csf Leaks
dc.subject
Chemotherapy
dc.subject
Growth Factors
dc.subject.classification
Inmunología
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dc.subject.classification
Medicina Básica
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dc.subject.classification
CIENCIAS MÉDICAS Y DE LA SALUD
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dc.title
Assessing the role of selected growth factors and cytostatic agents in an in vitro model of human dura mater healing
dc.type
info:eu-repo/semantics/article
dc.type
info:ar-repo/semantics/artículo
dc.type
info:eu-repo/semantics/publishedVersion
dc.date.updated
2018-01-11T13:46:15Z
dc.journal.volume
36
dc.journal.number
12
dc.journal.pagination
1040-1046
dc.journal.pais
Reino Unido
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dc.journal.ciudad
Londres
dc.description.fil
Fil: Goldschmidt, Ezequiel Darío. Hospital Italiano; Argentina
dc.description.fil
Fil: Ielpi, Marcelo. Hospital Italiano. Instituto de Ciencias Básicas y Medicina Experimental; Argentina
dc.description.fil
Fil: Loresi, Monica Alejandra. Hospital Italiano. Instituto de Ciencias Básicas y Medicina Experimental; Argentina
dc.description.fil
Fil: D'adamo, Maximiliano. Hospital Italiano. Instituto de Ciencias Básicas y Medicina Experimental; Argentina
dc.description.fil
Fil: Giunta, Diego Hernan. Hospital Italiano; Argentina
dc.description.fil
Fil: Carrizo, Antonio. Hospital Italiano; Argentina
dc.description.fil
Fil: Ajler, Pablo. Hospital Italiano; Argentina
dc.description.fil
Fil: Yampolsky, Claudio. Hospital Italiano; Argentina
dc.description.fil
Fil: Argibay, Pablo. Hospital Italiano. Instituto de Ciencias Básicas y Medicina Experimental; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
dc.journal.title
Neurological Research
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dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/url/http://www.tandfonline.com/doi/abs/10.1179/1743132814Y.0000000429
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/doi/http://dx.doi.org/10.1179/1743132814Y.0000000429
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