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dc.contributor.author
Yannarelli, Gustavo Gabriel
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dc.contributor.author
Tsoporis, James N.
dc.contributor.author
Desjardins, Jean Francois
dc.contributor.author
Wang, Xing Hua
dc.contributor.author
Pourdjabbar, Ali
dc.contributor.author
Viswanathan, Sowmya
dc.contributor.author
Parker, Thomas G.
dc.contributor.author
Keating, Armand
dc.date.available
2018-01-18T21:00:43Z
dc.date.issued
2013-11
dc.identifier.citation
Yannarelli, Gustavo Gabriel; Tsoporis, James N.; Desjardins, Jean Francois; Wang, Xing Hua; Pourdjabbar, Ali; et al.; Donor Mesenchymal Stromal Cells (MSCs) Undergo Variable Cardiac Reprogramming in Vivo and Predominantly Co-Express Cardiac and Stromal Determinants after Experimental Acute Myocardial Infarction; Springer; Stem Cell Reviews And Reports; 10; 2; 11-2013; 304-315
dc.identifier.issn
1550-8943
dc.identifier.uri
http://hdl.handle.net/11336/33892
dc.description.abstract
We previously showed the emergence of predominantly non-fused murine cells co-expressing cardiac and stromal determinants in co-cultures of murine mesenchymal stromal cells (MSCs) and rat embryonic cardiomyocytes. To determine whether a similar phenotype is detectable in vivo in ischemic myocardium, we infused green fluorescence protein (GFP)-marked MSCs intravenously into wild-type mice in an acute myocardial infarction (AMI) model generated by ischemia/reperfusion (I/R) or fixed coronary artery ligation. We found that infused GFP+ cells were confined strictly to ischemic areas and represented approximately 10% of total cellularity. We showed that over 60% of the cells co-expressed collagen type IV and troponin T or myosin heavy chain, characteristic of MSCs and cardiomyocytes, respectively, and were CD45(-). Nonetheless, up to 25% of the GFP+ donor cells expressed one of two cardiomyocyte markers, either myosin heavy chain or troponin T, in the absence of MSC determinants. We also observed a marked reduction in OCT4 expression in MSCs pre-infusion compared with those lodged in the myocardium, suggesting reduced stem cell properties. Despite the low frequency of lodged donor MSCs, left-ventricular end-diastolic pressure was significantly better in experimental versus saline animals for both AMI (12.10±1.81 vs. 20.50±1.53 mmHg, p=0.001) and I/R models (8.75±2.95 vs. 17.53±3.85 mmHg, p=0.004) when measured 21 days after MSC infusion and is consistent with a paracrine effect. Our data indicate that donor MSCs undergo variable degrees of cardiomyocyte reprogramming with the majority co-expressing cardiomyocyte and stromal markers. Further studies are needed to elucidate the factors mediating the extent of cardiomyocyte reprogramming and importance of the cellular changes on tissue repair.
dc.format
application/pdf
dc.language.iso
eng
dc.publisher
Springer
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dc.rights
info:eu-repo/semantics/openAccess
dc.rights.uri
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.subject
Mesenchymal Stromal Cells
dc.subject
Cardiomyocyte Reprograming
dc.subject
Acute Myocardial Infarction
dc.subject.classification
Inmunología
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dc.subject.classification
Medicina Básica
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dc.subject.classification
CIENCIAS MÉDICAS Y DE LA SALUD
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dc.title
Donor Mesenchymal Stromal Cells (MSCs) Undergo Variable Cardiac Reprogramming in Vivo and Predominantly Co-Express Cardiac and Stromal Determinants after Experimental Acute Myocardial Infarction
dc.type
info:eu-repo/semantics/article
dc.type
info:ar-repo/semantics/artículo
dc.type
info:eu-repo/semantics/publishedVersion
dc.date.updated
2018-01-16T18:04:31Z
dc.identifier.eissn
1558-6804
dc.journal.volume
10
dc.journal.number
2
dc.journal.pagination
304-315
dc.journal.pais
Estados Unidos
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dc.journal.ciudad
Nueva York
dc.description.fil
Fil: Yannarelli, Gustavo Gabriel. University of Toronto; Canadá. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
dc.description.fil
Fil: Tsoporis, James N.. St. Michael’s Hospital. Li Ka Shing Knowledge Institute. Keenan Research Centre. Department of Medicine. Division of Cardiology; Canadá
dc.description.fil
Fil: Desjardins, Jean Francois. St. Michael’s Hospital. Li Ka Shing Knowledge Institute. Keenan Research Centre. Department of Medicine. Division of Cardiology; Canadá
dc.description.fil
Fil: Wang, Xing Hua. University of Toronto; Canadá
dc.description.fil
Fil: Pourdjabbar, Ali. St. Michael’s Hospital. Li Ka Shing Knowledge Institute. Keenan Research Centre. Department of Medicine. Division of Cardiology; Canadá
dc.description.fil
Fil: Viswanathan, Sowmya. University of Toronto; Canadá
dc.description.fil
Fil: Parker, Thomas G.. St. Michael’s Hospital. Li Ka Shing Knowledge Institute. Keenan Research Centre. Department of Medicine. Division of Cardiology; Canadá
dc.description.fil
Fil: Keating, Armand. University of Toronto; Canadá
dc.journal.title
Stem Cell Reviews And Reports
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dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/url/https://link.springer.com/article/10.1007%2Fs12015-013-9483-y
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/doi/http://dx.doi.org/10.1007/s12015-013-9483-y
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