Show simple item record

dc.contributor.author Da Cruz Cabral, Lucía Mariana
dc.contributor.author Rodriguero, Marcela Silvina
dc.contributor.author Stenglein, Sebastian Alberto
dc.contributor.author Nielsen, Kristian Fog
dc.contributor.author Patriarca, Andrea Rosana
dc.date.available 2018-01-18T18:29:38Z
dc.date.issued 2017-09
dc.identifier.citation Da Cruz Cabral, Lucía Mariana; Rodriguero, Marcela Silvina; Stenglein, Sebastian Alberto; Nielsen, Kristian Fog; Patriarca, Andrea Rosana; Characterization of small-spored Alternaria from Argentinean crops through a polyphasic approach; Elsevier Science; International Journal of Food Microbiology; 257; 9-2017; 206-215
dc.identifier.issn 0168-1605
dc.identifier.uri http://hdl.handle.net/11336/33824
dc.description.abstract Small-spored Alternaria have been isolated from a wide variety of food crops, causing both economic losses and human health risk due to the metabolites produced. Their taxonomy has been discussed widely, but no scientific consensus has been established in this field to date. Argentina is a major exporter of agricultural products, so it is essential to thoroughly understand the physiological behaviour of this pathogen in a food safety context. Thus, the objective of this work was to characterize small-spored Alternaria spp. obtained from tomato fruits, pepper fruits, wheat grains and blueberries from Argentina by a polyphasic approach involving metabolomic and phylogenetic analyses based on molecular and morphological characters. Morphological analysis divided the population studied into three groups; A. arborescens sp.-grp., A. tenuissima sp.-grp., and A. alternata sp.-grp. However, when these characters were simultaneously analysed with molecular data, no clearly separated groups were obtained. Haplotype network and phylogenetic analysis (both Bayesian and maximum parsimony) of a conserved region yielded the same result, suggesting that all isolates belong to the same species. Furthermore, no correlation could be established between morphological species-groups and a metabolite or group of metabolites synthesized. Thus, the whole set of analyses carried out in the present work supports the hypothesis that these small-spored Alternaria isolates from food belong to the same species. Identification at species level through classical morphology or modern molecular techniques does not seem to be a useful tool to predict toxicological risk in food matrices. The detection of any small-spored Alternaria from Section Alternaria (D.P. Lawr., Gannibal, Peever & B.M. Pryor 2013) in food implies a potential toxicological risk.
dc.format application/pdf
dc.language.iso eng
dc.publisher Elsevier Science
dc.rights info:eu-repo/semantics/restrictedAccess
dc.rights Atribución-NoComercial-CompartirIgual 2.5 Argentina (CC BY-NC-SA 2.5 AR)
dc.rights.uri https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.subject ALTERNARIA
dc.subject ENDOPOLYGALACTURONASE GENE
dc.subject POLYPHASIC APPROACH
dc.subject METABOLITES PROFILE
dc.subject FOOD
dc.subject.classification Alimentos y Bebidas
dc.subject.classification Otras Ingenierías y Tecnologías
dc.subject.classification INGENIERÍAS Y TECNOLOGÍAS
dc.title Characterization of small-spored Alternaria from Argentinean crops through a polyphasic approach
dc.type info:eu-repo/semantics/article
dc.type info:ar-repo/semantics/artículo
dc.type info:eu-repo/semantics/publishedVersion
dc.date.updated 2017-12-19T18:48:49Z
dc.journal.volume 257
dc.journal.pagination 206-215
dc.journal.pais Países Bajos
dc.journal.ciudad Amsterdam
dc.description.fil Fil: Da Cruz Cabral, Lucía Mariana. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Química Orgánica; Argentina
dc.description.fil Fil: Rodriguero, Marcela Silvina. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Ecología, Genética y Evolución; Argentina
dc.description.fil Fil: Stenglein, Sebastian Alberto. Universidad Nacional del Centro de la Provincia de Buenos Aires. Facultad de Agronomía; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mar del Plata. Instituto de Investigaciones en Biodiversidad y Biotecnología; Argentina
dc.description.fil Fil: Nielsen, Kristian Fog. Technical University of Denmark; Dinamarca
dc.description.fil Fil: Patriarca, Andrea Rosana. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Química Orgánica; Argentina
dc.journal.title International Journal of Food Microbiology
dc.relation.alternativeid info:eu-repo/semantics/altIdentifier/url/http://www.sciencedirect.com/science/article/pii/S0168160517302945
dc.relation.alternativeid info:eu-repo/semantics/altIdentifier/doi/http://dx.doi.org/10.1016/j.ijfoodmicro.2017.06.026
dc.conicet.fuente Crossref


Archivos asociados

Icon
Blocked Acceso no disponible

This item appears in the following Collection(s)

Show simple item record

info:eu-repo/semantics/restrictedAccess Excepto donde se diga explícitamente, este item se publica bajo la siguiente descripción: Creative Commons Attribution-NonCommercial-ShareAlike 2.5 Unported (CC BY-NC-SA 2.5)