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dc.contributor.author
Marconi, Patricia Laura
dc.contributor.author
Alvarez, Maria Alejandra
dc.date.available
2018-01-17T20:45:34Z
dc.date.issued
2014-07
dc.identifier.citation
Marconi, Patricia Laura; Alvarez, Maria Alejandra; The expression of the 14D9 catalytic antibody in suspended cells of Nicotiana tabacum cultures increased by the addition of protein stabilizers and by transference from erlenmeyer flasks to a 2-L bioreactor; Wiley; Biotechnology Progress; 30; 5; 7-2014; 1185-1189
dc.identifier.issn
8756-7938
dc.identifier.uri
http://hdl.handle.net/11336/33713
dc.description.abstract
The effect of two protein stabilizers (polyvinylpyrrolidone [PVP] and gelatine) on growth and 14D9 yield of Nicotiana tabacum cell suspension cultures (Ab-KDEL and sec-Ab) was analyzed. The addition of PVP at a concentration of 1.0 g L−1 produced the highest total 14D9 yield (biomass + culture medium) in the Ab-KDEL line (4.82% total soluble protein [TSP]). With the addition of gelatine, the highest total 14D9 yield (2.48% TSP) was attained in the Ab-KDEL line at 5.0 g L−1 gelatine. When the Ab-KDEL suspended cells were cultured in a 2-L bioreactor, the highest 14D9 yield was 8.1% TSP at a 5% w/v inoculum size, which was the best 14D9 yield so far obtained in the platforms tested (E. coli, N. tabacum leaves and seeds, N. tabacum hairy roots, and cell suspension cultures).
dc.format
application/pdf
dc.language.iso
eng
dc.publisher
Wiley
dc.rights
info:eu-repo/semantics/openAccess
dc.rights.uri
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.subject
Catalytic Antibody 14d9
dc.subject
Cell Suspension Cultures
dc.subject
Hairy Roots
dc.subject
In Vitro Plant Cell Cultures
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Nicotiana Tabacum
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Protein Additives
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Bioreactor
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Otras Biotecnología Agropecuaria
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Biotecnología Agropecuaria
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CIENCIAS AGRÍCOLAS
dc.title
The expression of the 14D9 catalytic antibody in suspended cells of Nicotiana tabacum cultures increased by the addition of protein stabilizers and by transference from erlenmeyer flasks to a 2-L bioreactor
dc.type
info:eu-repo/semantics/article
dc.type
info:ar-repo/semantics/artículo
dc.type
info:eu-repo/semantics/publishedVersion
dc.date.updated
2018-01-16T18:02:01Z
dc.identifier.eissn
1520-6033
dc.journal.volume
30
dc.journal.number
5
dc.journal.pagination
1185-1189
dc.journal.pais
Estados Unidos
dc.journal.ciudad
Hoboken
dc.description.fil
Fil: Marconi, Patricia Laura. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Maimónides. Facultad de Ciencias de la Salud. Carreras de Farmacia y Bioquímica. Cátedra de Farmacobotánica y Farmacognoscia; Argentina
dc.description.fil
Fil: Alvarez, Maria Alejandra. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Maimónides. Facultad de Ciencias de la Salud. Carreras de Farmacia y Bioquímica. Cátedra de Farmacobotánica y Farmacognoscia; Argentina
dc.journal.title
Biotechnology Progress
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/url/http://onlinelibrary.wiley.com/doi/10.1002/btpr.1940/abstract
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/doi/http://dx.doi.org/10.1002/btpr.1940
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