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dc.contributor.author
Felitti, Silvina Andrea
dc.contributor.author
Acuña, Carlos Alberto
dc.contributor.author
Ortiz, Juan Pablo Amelio
dc.contributor.author
Quarin, Camilo Luis
dc.date.available
2016-01-06T14:50:11Z
dc.date.issued
2015-07
dc.identifier.citation
Felitti, Silvina Andrea; Acuña, Carlos Alberto; Ortiz, Juan Pablo Amelio; Quarin, Camilo Luis; Transcriptome analysis of seed development in apomictic Paspalum notatum; Wiley Blackwell Publishing, Inc; Annals of Applied Biology; 167; 1; 7-2015; 36-54
dc.identifier.issn
0003-4746
dc.identifier.uri
http://hdl.handle.net/11336/3369
dc.description.abstract
The seed developmental process involves various tissues with several ploidy levels and different genetic origins. Therefore, its characterisation at the transcriptome level is certainly a challenge. The hypothesis of endosperm balance number (EBN) postulates that each species has an effective number that is important for normal endosperm and seed development to occur. Understanding endosperm formation in apomictic plants is crucial for the perspective of transferring apomixis to sexual species of agronomic interest. Since sexual tetraploid Paspalum plants fit the EBN premise, the EBN insensitivity observed in apomictic plants might be a requirement for the spread of pseudogamous apomixis. Crosses using several cytotypes of Paspalum notatum were made in order to induce the development of seeds with different maternal/paternal genomic ratios in the endosperm. A transcriptome characterisation of ovaries 3 h after pollination was performed using cDNA-AFLP methodology. Forty-six of the 100 differentially expressed transcript-derived fragments (DETDFs) were specifically found in crosses in which apomictic plants were used as the female parent and presented a predicted m: p ratio in the endosperm that was different to the 2:1 requirement of the EBN. Moreover, 12 of the DETDFs presented identity with proteins that were differentially expressed in response to changes in the levels of extracellular ATP (eATP) in Arabidopsis cell suspension cultures. eATP is an important molecular switch in plants that tightly controls organellar energy metabolism and activates gene expression controlling specific growth and developmental programmes. The results suggest that eATP-mediated signalling could be involved in the regulation of endosperm development.
dc.format
application/pdf
dc.language.iso
eng
dc.publisher
Wiley Blackwell Publishing, Inc
dc.rights
info:eu-repo/semantics/openAccess
dc.rights.uri
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.subject
Endosperm
dc.subject
Endosperm Balance Number
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Extracellular Atp
dc.subject
Paspalum Notatum
dc.subject
Pseudogamy
dc.subject
Transcriptomics
dc.subject.classification
Genética y Herencia
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Ciencias Biológicas
dc.subject.classification
CIENCIAS NATURALES Y EXACTAS
dc.subject.classification
Ciencias de las Plantas, Botánica
dc.subject.classification
Ciencias Biológicas
dc.subject.classification
CIENCIAS NATURALES Y EXACTAS
dc.title
Transcriptome analysis of seed development in apomictic Paspalum notatum
dc.type
info:eu-repo/semantics/article
dc.type
info:ar-repo/semantics/artículo
dc.type
info:eu-repo/semantics/publishedVersion
dc.date.updated
2016-03-30 10:35:44.97925-03
dc.journal.volume
167
dc.journal.number
1
dc.journal.pagination
36-54
dc.journal.pais
Reino Unido
dc.journal.ciudad
Londres
dc.description.fil
Fil: Felitti, Silvina Andrea. Universidad Nacional de Rosario. Facultad de Ciencias Agrarias. Laboratorio de Biologia Molecular; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Cientifico Tecnológico Rosario; Argentina
dc.description.fil
Fil: Acuña, Carlos Alberto. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Nordeste. Instituto de Botánica del Nordeste (i); Argentina
dc.description.fil
Fil: Ortiz, Juan Pablo Amelio. Universidad Nacional de Rosario. Facultad de Ciencias Agrarias. Laboratorio de Biologia Molecular; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Nordeste. Instituto de Botánica del Nordeste (i); Argentina
dc.description.fil
Fil: Quarin, Camilo Luis. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Nordeste. Instituto de Botánica del Nordeste (i); Argentina
dc.journal.title
Annals of Applied Biology
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/doi/http://dx.doi.org/DOI:10.1111/aab.12206
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/url/https://onlinelibrary.wiley.com/doi/10.1111/aab.12206
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