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dc.contributor.author
Arena, Miriam Elisabet
dc.contributor.author
Martínez Pastur, Guillermo José
dc.contributor.author
Benavides, Maria Patricia
dc.contributor.author
Zappacosta, Diego Carlos
dc.contributor.author
Eliasco, Eleonora
dc.contributor.author
Curvetto, Nestor Raul
dc.date.available
2018-01-17T17:23:45Z
dc.date.issued
2003-12
dc.identifier.citation
Arena, Miriam Elisabet; Curvetto, Nestor Raul; Zappacosta, Diego Carlos; Benavides, Maria Patricia; Martínez Pastur, Guillermo José; Eliasco, Eleonora; et al.; Peroxidase and polyamine activity variation during the in vitro rooting of Berberis buxifolia; Rsnz Publishing; New Zealand Journal of Botany; 41; 3; 12-2003; 475-485
dc.identifier.issn
0028-825X
dc.identifier.uri
http://hdl.handle.net/11336/33625
dc.description.abstract
Berberis buxifolia is a Patagonian shrub with great economic potential for tinctorial, pharmacological, and food industries. Clonal propagation is possible through in vitro culture and is also useful for metabolite production. However, this species is difficult to root, and to improve this, more knowledge of rhizogenesis processes is needed. Polyamines and peroxidases are useful biochemical markers during analysis of rooting phases for correlation with tissue morphological changes. Therefore, endogenous polyamine (putrescine, spermidine, spermine) changes, peroxidase activity evolution, and morphological development were studied to characterise the in vitro rhizogenesis of microshoots of B. buxifolia and, thus, to define the rooting phases. Polyamine and peroxidase changed significantly during the rooting period, and had opposite behaviours which were directly related to the IBA media content. The lower polyamine concentration and the higher peroxidase activity were found in a treatment with a dark period during the first four days and with IBA in the culture medium. Putrescine was the most abundant polyamine found in B. buxifolia tissues, 14- to 18-fold more than spermidine and spermine, respectively. Therefore, these compounds were used to define the rooting phases: an induction phase (0 to 4–7 days) followed by an expression phase (4–7 to 28 days). The observed changes in the biochemical markers could be correlated with microscopic and macroscopic tissue observations in the microshoots, and the time course of rooting percentage. Successive culture media can be developed including polyamines, or other compounds and environmental conditions, which positively modify the studied biochemical markers behaviour.
dc.format
application/pdf
dc.language.iso
eng
dc.publisher
Rsnz Publishing
dc.rights
info:eu-repo/semantics/restrictedAccess
dc.rights.uri
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.subject
Rhizogenesis
dc.subject
Rooting Markers
dc.subject
Histology
dc.subject
Micropropagation
dc.subject
Patagonia
dc.subject
Berberis Buxifolia
dc.subject.classification
Otras Biotecnología Agropecuaria
dc.subject.classification
Biotecnología Agropecuaria
dc.subject.classification
CIENCIAS AGRÍCOLAS
dc.title
Peroxidase and polyamine activity variation during the in vitro rooting of Berberis buxifolia
dc.type
info:eu-repo/semantics/article
dc.type
info:ar-repo/semantics/artículo
dc.type
info:eu-repo/semantics/publishedVersion
dc.date.updated
2017-11-16T15:05:58Z
dc.journal.volume
41
dc.journal.number
3
dc.journal.pagination
475-485
dc.journal.pais
Nueva Zelanda
dc.journal.ciudad
Wellington
dc.description.fil
Fil: Arena, Miriam Elisabet. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Austral de Investigaciones Científicas; Argentina
dc.description.fil
Fil: Martínez Pastur, Guillermo José. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Austral de Investigaciones Científicas; Argentina
dc.description.fil
Fil: Benavides, Maria Patricia. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
dc.description.fil
Fil: Zappacosta, Diego Carlos. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Centro de Recursos Naturales Renovables de la Zona Semiárida. Universidad Nacional del Sur. Centro de Recursos Naturales Renovables de la Zona Semiárida; Argentina
dc.description.fil
Fil: Eliasco, Eleonora. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Austral de Investigaciones Científicas; Argentina
dc.description.fil
Fil: Curvetto, Nestor Raul. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Centro de Recursos Naturales Renovables de la Zona Semiárida. Universidad Nacional del Sur. Centro de Recursos Naturales Renovables de la Zona Semiárida; Argentina
dc.journal.title
New Zealand Journal of Botany
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/url/http://www.tandfonline.com/doi/abs/10.1080/0028825X.2003.9512864
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/doi/http://dx.doi.org/10.1080/0028825X.2003.9512864


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  • Articulos(CADIC) [669]
    Articulos de CENTRO AUSTRAL DE INVESTIGACIONES CIENTIFICAS
  • Articulos(CERZOS) [474]
    Articulos de CENTRO REC.NAT.RENOVABLES DE ZONA SEMIARIDA(I)

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