Artículo
Role of UTX in Retinoic Acid Receptor-Mediated Gene Regulation in Leukemia
Rocha Viegas, Luciana
; Villa, Raffaella; Gutierrez, Arantxa; Iriondo, Oihana; Shiekhattar, Ramin; Di Croce, Luciano
Fecha de publicación:
10/2014
Editorial:
American Society for Microbiology
Revista:
Molecular and Cellular Biology
ISSN:
0270-7306
Idioma:
Inglés
Tipo de recurso:
Artículo publicado
Clasificación temática:
Resumen
Human UTX, a member of the Jumonji C family of proteins, associates with mixed-lineage leukemia 3/4 complexes. Stimulation with retinoic acid leads to the recruitment of UTX-containing complexes to HOX genes, which results in demethylation of histone H3 lysine 27 and concomitant methylation of histone H3 lysine 4. Here, we show that UTX interacts with the retinoic acid receptor α (RARα) and that this interaction is essential for proper differentiation of leukemic U937 cells in response to retinoic acid. UTX occupies the promoters of several RAR target genes and regulates their transcriptional output by modulating ASH2L complex recruitment. Overexpression of UTX in promyelocytic NB4 cells results in enhanced cellular differentiation upon retinoic acid treatment. Our results show that UTX is important for RAR-mediated transcription and provide insight into the critical role of cross talk between histone H3 lysine 4 methylation and histone H3 lysine 27 demethylation during cellular differentiation.
Palabras clave:
Utx
,
Rar
,
Leukemia
,
Differentiation
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Articulos(IFIBYNE)
Articulos de INST.DE FISIOL., BIOL.MOLECULAR Y NEUROCIENCIAS
Articulos de INST.DE FISIOL., BIOL.MOLECULAR Y NEUROCIENCIAS
Citación
Rocha Viegas, Luciana; Shiekhattar, Ramin; Di Croce, Luciano; Iriondo, Oihana; Gutierrez, Arantxa; Villa, Raffaella; et al.; Role of UTX in Retinoic Acid Receptor-Mediated Gene Regulation in Leukemia; American Society for Microbiology; Molecular and Cellular Biology; 34; 19; 10-2014; 3765-3775
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