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dc.contributor.author
Pappalardo, Juan Sebastian
dc.contributor.author
Langellotti, Cecilia Ana
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Di Giacomo, Sebastián
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Olivera, Valeria
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Quattrocchi, Valeria
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Zamorano, Patricia Ines
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Hartner, William C.
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Levchenko, Tatyana S.
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Torchilin, Vladimir P.
dc.date.available
2018-01-15T19:47:58Z
dc.date.issued
2014-02
dc.identifier.citation
Olivera, Valeria; Di Giacomo, Sebastián; Pappalardo, Juan Sebastian; Levchenko, Tatyana S.; Quattrocchi, Valeria; Hartner, William C.; et al.; In vitro transfection of bone marrow-derived dendritic cells with TATp-liposomes; Dove Press; International Journal of Nanomedicine; 9; 1; 2-2014; 963-973
dc.identifier.issn
1176-9114
dc.identifier.uri
http://hdl.handle.net/11336/33327
dc.description.abstract
Dendritic cells (DC) are antigen presenting cells (APC) uniquely capable of priming naïve T cells and cross-presenting antigens and determine the type of immune response elicited against an antigen. TAT peptide (TATp), is an amphipathic, arginine-rich, cationic peptide that promotes penetration and translocation of various molecules and nanoparticles into cells. TATp-liposomes (TATp-L) used for DC transfection were prepared using TATp derivatized with a lipid-terminated polymer capable of anchoring in the liposomal membrane. Here, we show that the addition of TATp to DNA-loaded liposomes increased the uptake of DNA in DC. DNA-loaded TATp-L increased the in vitro transfection efficiency in DC cultures as evidenced by an enhanced expression of the enhanced green fluorescent protein (EGFP) and bovine herpes virus type 1 (BoHV-1) glycoprotein D (gD). The de novo synthesized gD protein was immunologically stimulating when transfections were performed with TATp-L as indicated by the secretion of interleukin 6 (IL-6).
dc.format
application/pdf
dc.language.iso
eng
dc.publisher
Dove Press
dc.rights
info:eu-repo/semantics/openAccess
dc.rights.uri
https://creativecommons.org/licenses/by-nc/2.5/ar/
dc.subject
Tat Peptide
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Bovine Herpes Virus 1 Glycoprotein D
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Dendritic Cell Transfection
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Green Fluorescent Protein
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Nano-materiales
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Nanotecnología
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INGENIERÍAS Y TECNOLOGÍAS
dc.title
In vitro transfection of bone marrow-derived dendritic cells with TATp-liposomes
dc.type
info:eu-repo/semantics/article
dc.type
info:ar-repo/semantics/artículo
dc.type
info:eu-repo/semantics/publishedVersion
dc.date.updated
2018-01-11T13:48:07Z
dc.identifier.eissn
1178-2013
dc.journal.volume
9
dc.journal.number
1
dc.journal.pagination
963-973
dc.journal.pais
Nueva Zelanda
dc.journal.ciudad
Auckland
dc.description.fil
Fil: Pappalardo, Juan Sebastian. Northeastern University. Center for Pharmaceutical Biotechnology and Nanomedicine; Estados Unidos. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigación en Ciencias Veterinarias y Agronómicas. Instituto de Virología; Argentina
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Fil: Langellotti, Cecilia Ana. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
dc.description.fil
Fil: Di Giacomo, Sebastián. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigación en Ciencias Veterinarias y Agronómicas. Instituto de Virología; Argentina
dc.description.fil
Fil: Olivera, Valeria. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigación en Ciencias Veterinarias y Agronómicas. Instituto de Virología; Argentina
dc.description.fil
Fil: Quattrocchi, Valeria. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
dc.description.fil
Fil: Zamorano, Patricia Ines. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigación en Ciencias Veterinarias y Agronómicas. Instituto de Virología; Argentina
dc.description.fil
Fil: Hartner, William C.. Northeastern University. Center for Pharmaceutical Biotechnology and Nanomedicine; Estados Unidos
dc.description.fil
Fil: Levchenko, Tatyana S.. Northeastern University. Center for Pharmaceutical Biotechnology and Nanomedicine; Estados Unidos
dc.description.fil
Fil: Torchilin, Vladimir P.. Northeastern University. Center for Pharmaceutical Biotechnology and Nanomedicine; Estados Unidos
dc.journal.title
International Journal of Nanomedicine
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/url/https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3928453/
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info:eu-repo/semantics/altIdentifier/doi/http://dx.doi.org/10.2147/IJN.S53432
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/url/https://www.dovepress.com/in-vitro-transfection-of-bone-marrow-derived-dendritic-cells-with-tatp-peer-reviewed-article-IJN