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dc.contributor.author
Nikoloff, Noelia
dc.contributor.author
Larramendy, Marcelo Luis
dc.contributor.author
Soloneski, Sonia Maria Elsa
dc.date.available
2018-01-10T19:01:15Z
dc.date.issued
2014-10
dc.identifier.citation
Nikoloff, Noelia; Larramendy, Marcelo Luis; Soloneski, Sonia Maria Elsa; Comparative EvaluationIn vitroof the HerbicideFlurochloridone by Cytokinesis-blockMicronucleus Cytome and Comet Assays; John Wiley & Sons Inc; Environmental Toxicology; 29; 8; 10-2014; 884-892
dc.identifier.issn
1520-4081
dc.identifier.uri
http://hdl.handle.net/11336/32872
dc.description.abstract
Thein-vitroeffects of flurochloridone and its formulations Twin Pack Gold1(25% a.i.) andRainbow1(25% a.i.) were evaluated in Chinese Hamster Ovary K1 (CHO-K1) cells. The cytokinesis-blockmicronucleus cytome (CBMN-cyt) and single-cell gel electrophoresis (SCGE) assays were used. Theactivities were tested within the range of final concentrations of 0.25–15lg flurochloridone/mL. Theresults demonstrated that both the flurochloridone and Rainbow1were not able to induce micronuclei(MN). On the other hand, Twin Pack Gold1only increased the frequency of MN at 5lg/mL. Furthermore,10 and 15lg/mL of both formulations resulted in a cellular cytotoxicity demonstrated by alterations in thenuclear division index and cellular death. SCGE assay appeared to be a more sensitive bioassay fordetecting primary DNA strand breaks at lower concentrations of flurochloridone than MN did. A markedincrease in the genetic damage index was observed when 5 and 15lg/mL of both flurochloridone andRainbow1but only when 15lg/mL of Twin Pack Gold1were used. This is the first report demonstratingthat flurochloridone and its two commercial formulations are able to induce single-strand DNA breaksinvitroon mammalian cells.
dc.format
application/pdf
dc.language.iso
eng
dc.publisher
John Wiley & Sons Inc
dc.rights
info:eu-repo/semantics/openAccess
dc.rights.uri
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.subject
Flurochloridone
dc.subject
Cbmn-Cyt Assay
dc.subject
Dna-Single Strands Breaks
dc.subject
Cho-K1 Cells
dc.subject
Commercial Formulations
dc.subject.classification
Otras Ciencias Biológicas
dc.subject.classification
Ciencias Biológicas
dc.subject.classification
CIENCIAS NATURALES Y EXACTAS
dc.title
Comparative EvaluationIn vitroof the HerbicideFlurochloridone by Cytokinesis-blockMicronucleus Cytome and Comet Assays
dc.type
info:eu-repo/semantics/article
dc.type
info:ar-repo/semantics/artículo
dc.type
info:eu-repo/semantics/publishedVersion
dc.date.updated
2018-01-10T16:51:34Z
dc.journal.volume
29
dc.journal.number
8
dc.journal.pagination
884-892
dc.journal.pais
Estados Unidos
dc.journal.ciudad
New York
dc.description.fil
Fil: Nikoloff, Noelia. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional de La Plata. Facultad de Ciencias Naturales y Museo. Cátedra de Citología; Argentina
dc.description.fil
Fil: Larramendy, Marcelo Luis. Universidad Nacional de La Plata. Facultad de Ciencias Naturales y Museo. Cátedra de Citología; Argentina
dc.description.fil
Fil: Soloneski, Sonia Maria Elsa. Universidad Nacional de La Plata. Facultad de Ciencias Naturales y Museo. Cátedra de Citología; Argentina
dc.journal.title
Environmental Toxicology
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/doi/http://dx.doi.org/10.1002/tox.21816
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/url/http://onlinelibrary.wiley.com/doi/10.1002/tox.21816/abstract
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