Artículo
Studies on PVA pectin cryogels containing crosslinked enzyme aggregates of keratinase
Martinez, Yanina N.; Cavello, Ivana Alejandra
; Cavalitto, Sebastian Fernando
; Illanes, Andres; Castro, Guillermo Raul
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Fecha de publicación:
02/2014
Editorial:
Elsevier Science
Revista:
Colloids and Surfaces B: Biointerfaces
ISSN:
0927-7765
Idioma:
Inglés
Tipo de recurso:
Artículo publicado
Clasificación temática:
Resumen
Polyvinyl alcohol-pectin (PVA-P) films containing enrofloxacin and keratinase were developed to treat wounds and scars produced by burns and skin injuries. However, in order to prevent enzyme inactivation at the interface between the patch and the scars, crosslinked enzyme aggregates (CLEAs) from a crude extract of keratinase produced by Paecilomyces lilacinus (LPSC#876) were synthesized by precipitation with acetone and crosslinking with glutaraldehyde. Soluble vs. CLEA keratinase (K-CLEA) activities were tested in 59% (v/v) hydrophobic (isobutanol and n-hexane) and hydrophilic (acetone and dimethylsulfoxide) solvents mixtures. K-CLEA activity was 1.4, 1.7 and 6.6 times higher in acetone, n-hexane and isobutanol than the soluble enzyme at 37 °C after 1 h of incubation, respectively. K-CLEA showed at least 45% of enzyme residual activity in the 40–65 °C range, meanwhile the soluble biocatalyst was fully inactivated at 65 °C after 1 h incubation. Also, the soluble enzyme was completely inactivated after 12 h at pH 7.4 and 45 °C, even though K-CLEA retained full activity. The soluble keratinase was completely inactivated at 37 °C after storage in buffer solution (pH 7.4) for 2 months, meanwhile K-CLEAs kept 51% of their activity. K-CLEA loaded into polyvinyl alcohol (PVA) and PVA-P cryogels showed six times lower release rate compared to the soluble keratinase at skin pH (5.5). Small angle X-ray scattering (SAXS) analysis showed that K-CLEA bound to pectin rather than to PVA in the PVA-P matrix.
Palabras clave:
Clea
,
Pva
,
Keratinase
,
Controlled Release
,
Cryogel
,
Autolysis
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Identificadores
Colecciones
Articulos(CINDEFI)
Articulos de CENT.DE INV EN FERMENTACIONES INDUSTRIALES (I)
Articulos de CENT.DE INV EN FERMENTACIONES INDUSTRIALES (I)
Citación
Castro, Guillermo Raul; Illanes, Andres; Cavalitto, Sebastian Fernando; Cavello, Ivana Alejandra; Martinez, Yanina N.; Studies on PVA pectin cryogels containing crosslinked enzyme aggregates of keratinase; Elsevier Science; Colloids and Surfaces B: Biointerfaces; 117; 2-2014; 284-289
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