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dc.contributor.author
Althabegoiti, Maria Julia  
dc.contributor.author
Ormeño Orrillo, Ernesto  
dc.contributor.author
Lozano, Luis  
dc.contributor.author
Torres Tejerizo, Gonzalo Arturo  
dc.contributor.author
Rogel, Marco Antonio  
dc.contributor.author
Mora, Jaime  
dc.contributor.author
Martinez Romero, Esperanza  
dc.date.available
2018-01-04T19:05:18Z  
dc.date.issued
2014-01  
dc.identifier.citation
Martinez Romero, Esperanza; Mora, Jaime; Rogel, Marco Antonio; Torres Tejerizo, Gonzalo Arturo; Lozano, Luis; Ormeño Orrillo, Ernesto; et al.; Characterization of Rhizobium grahamii extrachromosomal replicons and their transfer among rhizobia; BioMed Central; Bmc Microbiology; 14; 6; 1-2014; 1-10  
dc.identifier.issn
1471-2180  
dc.identifier.uri
http://hdl.handle.net/11336/32348  
dc.description.abstract
Background Rhizobium grahamii belongs to a new phylogenetic group of rhizobia together with Rhizobium mesoamericanum and other species. R. grahamii has a broad-host-range that includes Leucaena leucocephala and Phaseolus vulgaris, although it is a poor competitor for P. vulgaris nodulation in the presence of Rhizobium etli or Rhizobium phaseoli strains. This work analyzed the genome sequence and transfer properties of R. grahamii plasmids. Results Genome sequence was obtained from R. grahamii CCGE502 type strain isolated from Dalea leporina in Mexico. The CCGE502 genome comprises one chromosome and two extrachromosomal replicons (ERs), pRgrCCGE502a and pRgrCCGE502b. Additionally, a plasmid integrated in the CCGE502 chromosome was found. The genomic comparison of ERs from this group showed that gene content is more variable than average nucleotide identity (ANI). Well conserved nod and nif genes were found in R. grahamii and R. mesoamericanum with some differences. R. phaseoli Ch24-10 genes expressed in bacterial cells in roots were found to be conserved in pRgrCCGE502b. Regarding conjugative transfer we were unable to transfer the R. grahamii CCGE502 symbiotic plasmid and its megaplasmid to other rhizobial hosts but we could transfer the symbiotic plasmid to Agrobacterium tumefaciens with transfer dependent on homoserine lactones. Conclusion Variable degrees of nucleotide identity and gene content conservation were found among the different R. grahamii CCGE502 replicons in comparison to R. mesoamericanum genomes. The extrachromosomal replicons from R. grahamii were more similar to those found in phylogenetically related Rhizobium species. However, limited similarities of R. grahamii CCGE502 symbiotic plasmid and megaplasmid were observed in other more distant Rhizobium species. The set of conserved genes in R. grahamii comprises some of those that are highly expressed in R. phaseoli on plant roots, suggesting that they play an important role in root colonization.  
dc.format
application/pdf  
dc.language.iso
eng  
dc.publisher
BioMed Central  
dc.rights
info:eu-repo/semantics/openAccess  
dc.rights.uri
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/  
dc.subject
Genome Sequence  
dc.subject
Quorum Sensing  
dc.subject
Conjugative Transfer  
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Symbiotic Plasmid  
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Otras Ciencias Biológicas  
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Ciencias Biológicas  
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CIENCIAS NATURALES Y EXACTAS  
dc.title
Characterization of Rhizobium grahamii extrachromosomal replicons and their transfer among rhizobia  
dc.type
info:eu-repo/semantics/article  
dc.type
info:ar-repo/semantics/artículo  
dc.type
info:eu-repo/semantics/publishedVersion  
dc.date.updated
2018-01-03T19:05:12Z  
dc.journal.volume
14  
dc.journal.number
6  
dc.journal.pagination
1-10  
dc.journal.pais
Reino Unido  
dc.journal.ciudad
Londres  
dc.description.fil
Fil: Althabegoiti, Maria Julia. Universidad Nacional Autónoma de México; México. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina  
dc.description.fil
Fil: Ormeño Orrillo, Ernesto. Universidad Nacional Autónoma de México; México  
dc.description.fil
Fil: Lozano, Luis. Universidad Nacional Autónoma de México; México  
dc.description.fil
Fil: Torres Tejerizo, Gonzalo Arturo. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional Autónoma de México; México  
dc.description.fil
Fil: Rogel, Marco Antonio. Universidad Nacional Autónoma de México; México  
dc.description.fil
Fil: Mora, Jaime. Universidad Nacional Autónoma de México; México  
dc.description.fil
Fil: Martinez Romero, Esperanza. Universidad Nacional Autónoma de México; México  
dc.journal.title
Bmc Microbiology  
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/url/https://bmcmicrobiol.biomedcentral.com/articles/10.1186/1471-2180-14-6  
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/doi/http://dx.doi.org/10.1186/1471-2180-14-6