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Artículo

Water and Membrane Dynamics in Suspensions of Lipid Vesicles Functionalized with Poly(ethylene glycol)s

Clop, Eduardo MatiasIcon ; Chattah, Ana KarinaIcon ; Perillo, Maria AngelicaIcon
Fecha de publicación: 05/2014
Editorial: American Chemical Society
Revista: Journal of Physical Chemistry B
ISSN: 1520-6106
Idioma: Inglés
Tipo de recurso: Artículo publicado
Clasificación temática:
Otras Ciencias Biológicas

Resumen

The present work was aimed at studying the molecular dynamics at different levels of model membranes having a simulated glycoclix, with focus on the molecular crowding conditions at the lipid–water interfacial region. Thus, binary mixtures of dipalmitoylphosphatidylcholine (dpPC) and a poly(ethylene glycol) (PEGn) derivative of dipalmitoylphosphatidylethanolamine (PE) (where n = 350, 1000, and 5000, respectively, refer to PEG molecular masses) were submitted to 1H spin–lattice relaxation time (T1) and 31P NMR spectra analysis. 1H NMR relaxation times revealed two contributing components in each proton system (PEG, phospholipids, and water), for all the mixtures studied, exhibiting values of T1 with very different orders of magnitude. This allowed identifying confined and bulk water populations as well as PEG moieties becoming more disordered and independent from the phospholipid moiety as n increased. 31P spectra showed a typical broad bilayer signal for n = 350 and 1000, and an isotropic signal characteristic of micelles for n = 5000. Surface pressure (π)–molecular area isotherms and compressional modulus measurements provided further structural information. Moreover, epifluorescence microscopy data from monolayers at π ∼ 30 mN/m, the expected equilibrium π in lipid bilayers, allowed us to postulate that both 1H populations resolved through NMR in phospholipids and lipopolymers corresponded to different phase domains.
Palabras clave: Polymer Grafted Lipids , Pe-Peg , Water Dynamics
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info:eu-repo/semantics/openAccess Excepto donde se diga explícitamente, este item se publica bajo la siguiente descripción: Creative Commons Attribution-NonCommercial-ShareAlike 2.5 Unported (CC BY-NC-SA 2.5)
Identificadores
URI: http://hdl.handle.net/11336/32261
DOI: http://dx.doi.org/10.1021/jp410894x
URL: http://pubs.acs.org/doi/10.1021/jp410894x
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Articulos(IIBYT)
Articulos de INSTITUTO DE INVESTIGACIONES BIOLOGICAS Y TECNOLOGICAS
Citación
Perillo, Maria Angelica; Chattah, Ana Karina; Clop, Eduardo Matias; Water and Membrane Dynamics in Suspensions of Lipid Vesicles Functionalized with Poly(ethylene glycol)s; American Chemical Society; Journal of Physical Chemistry B; 118; 23; 5-2014; 6150-6158
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