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dc.contributor.author Elola, Maria Teresa
dc.contributor.author Capurro, Mariana Isabel
dc.contributor.author Barrio, Maria Marcela
dc.contributor.author Coombs, Peter J.
dc.contributor.author Taylor, Maureen E.
dc.contributor.author Drickamer, Kurt
dc.contributor.author Mordoh, Jose
dc.date.available 2018-01-03T20:52:24Z
dc.date.issued 2007-01
dc.identifier.citation Barrio, Maria Marcela; Capurro, Mariana Isabel; Elola, Maria Teresa; Mordoh, Jose; Drickamer, Kurt; Taylor, Maureen E.; et al.; Lewis X antigen mediates adhesion of human breast carcinoma cells to activated endothelium. Possible involvement of the endothelial scavenger receptor C-Type lectin; Springer; Breast Cancer Research and Treatment; 101; 2; 1-2007; 161-174
dc.identifier.issn 0167-6806
dc.identifier.uri http://hdl.handle.net/11336/32221
dc.description.abstract Lewis x (Lex, CD15), also known as SSEA-1 (stage specific embryonic antigen-1), is a trisaccharide with the structure Galβ(1–4)Fucα(1–3)GlcNAc, which is expressed on glycoconjugates in human polymorphonuclear granulocytes and various tumors such as colon and breast carcinoma. We have investigated the role of Lex in the adhesion of MCF-7 human breast cancer cells and PMN to human umbilical endothelial cells (HUVEC) and the effects of two different anti-Lex mAbs (FC-2.15 and MCS-1) on this adhesion. We also analyzed the cytolysis of Lex+-cells induced by anti-Lex mAbs and complement when cells were adhered to the endothelium, and the effect of these antibodies on HUVEC. The results indicate that MCF-7 cells can bind to HUVEC, and that MCS-1 but not FC-2.15 mAb inhibit this interaction. Both mAbs can efficiently lyse MCF-7 cells bound to HUVEC in the presence of complement without damaging endothelial cells. We also found a Lex-dependent PMN interaction with HUVEC. Although both anti-Lex mAbs lysed PMN in suspension and adhered to HUVEC, PMN aggregation was only induced by mAb FC-2.15. Blotting studies revealed that the endothelial scavenger receptor C-type lectin (SRCL), which binds Lex-trisaccharide, interacts with specific glycoproteins of Mr␣∼␣28 kD and 10 kD from MCF-7 cells. The interaction between Lex+-cancer cells and vascular endothelium is a potential target for cancer treatment.
dc.format application/pdf
dc.language.iso eng
dc.publisher Springer
dc.rights info:eu-repo/semantics/openAccess
dc.rights.uri https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.subject.classification Medicina Critica y de Emergencia
dc.subject.classification Medicina Clínica
dc.subject.classification CIENCIAS MÉDICAS Y DE LA SALUD
dc.title Lewis X antigen mediates adhesion of human breast carcinoma cells to activated endothelium. Possible involvement of the endothelial scavenger receptor C-Type lectin
dc.type info:eu-repo/semantics/article
dc.type info:ar-repo/semantics/artículo
dc.type info:eu-repo/semantics/publishedVersion
dc.date.updated 2017-12-12T20:03:47Z
dc.journal.volume 101
dc.journal.number 2
dc.journal.pagination 161-174
dc.journal.pais Alemania
dc.journal.ciudad Berlín
dc.description.fil Fil: Elola, Maria Teresa. Fundación Instituto Leloir; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
dc.description.fil Fil: Capurro, Mariana Isabel. University of Toronto; Canadá
dc.description.fil Fil: Barrio, Maria Marcela. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Fundación para la Investigación, Docencia y Prevención del Cáncer; Argentina
dc.description.fil Fil: Coombs, Peter J.. Imperial College London; Reino Unido
dc.description.fil Fil: Taylor, Maureen E.. Imperial College London; Reino Unido
dc.description.fil Fil: Drickamer, Kurt. Imperial College London; Reino Unido
dc.description.fil Fil: Mordoh, Jose. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Fundación para la Investigación, Docencia y Prevención del Cáncer; Argentina
dc.journal.title Breast Cancer Research and Treatment
dc.relation.alternativeid info:eu-repo/semantics/altIdentifier/url/https://link.springer.com/article/10.1007/s10549-006-9286-9
dc.relation.alternativeid info:eu-repo/semantics/altIdentifier/doi/https://doi.org/10.1007/s10549-006-9286-9
dc.relation.alternativeid info:eu-repo/semantics/altIdentifier/url/https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2288708/


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  • Articulos(IIBBA) [753]
    Articulos de INST.DE INVEST.BIOQUIMICAS DE BS.AS(I)
  • Articulos(IQUIFIB) [328]
    Articulos de INST.DE QUIMICA Y FISICO-QUIMICA BIOLOGICAS "PROF. ALEJANDRO C. PALADINI"

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info:eu-repo/semantics/openAccess Excepto donde se diga explícitamente, este item se publica bajo la siguiente descripción: Creative Commons Attribution-NonCommercial-ShareAlike 2.5 Unported (CC BY-NC-SA 2.5)