Artículo
VIP contribution to the decidualization program: regulatory T cell recruitment
Grasso, Esteban Nicolas
; Paparini, Daniel Esteban
; Agüero, Mariana; Mor, Gil; Perez Leiros, Claudia
; Ramhorst, Rosanna Elizabeth
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Fecha de publicación:
04/2014
Editorial:
BioScientifica
Revista:
Journal of Endocrinology
ISSN:
0022-0795
Idioma:
Inglés
Tipo de recurso:
Artículo publicado
Clasificación temática:
Resumen
During early pregnancy, the human uterus undergoes profound tissue remodeling characterized by leukocyte invasion and production of proinflammatory cytokines, followed by tissue repair and tolerance maintenance induction. Vasoactive intestinal peptide (VIP) is produced by trophoblast cells and modulates the maternal immune response toward a tolerogenic profile. Here, we evaluated the contribution of the VIP/VPAC to endometrial renewal, inducing decidualization and the recruitment of induced regulatory T cells (iTregs) that accompany the implantation period. For that purpose, we used an in vitro model of decidualization with a human endometrial stromal cell line (HESC) stimulated with progesterone (P4) and lipopolysaccharide (LPS) simulating the inflammatory response during implantation and human iTregs (CD4+CD25+FOXP3+) differentiated from naïve T cells obtained from peripheral blood mononuclear cells of fertile women. We observed that VIP and its receptor VPAC1 are constitutively expressed in HESCs and that P4 increased VIP expression. Moreover, in HESC VIP induced expression of RANTES (CCL5), one of the main chemokines involved in T cell recruitment, and this effect is enhanced by the presence of P4 and LPS. Finally, assays of the migration of iTregs toward conditioned media from HESCs revealed that endogenous VIP production induced by P4 and LPS and RANTES production were involved, as anti-RANTES neutralizing Ab or VIP antagonist prevented their migration. We conclude that VIP may have an active role in the decidualization process, thus contributing to recruitment of iTregs toward endometrial stromal cells by increasing RANTES expression in a P4-dependent manner.
Palabras clave:
Decidualization
,
Vip
,
Early Pregnancy
,
Human Endometrial Stromal Cells
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Colecciones
Articulos(IQUIBICEN)
Articulos de INSTITUTO DE QUIMICA BIOLOGICA DE LA FACULTAD DE CS. EXACTAS Y NATURALES
Articulos de INSTITUTO DE QUIMICA BIOLOGICA DE LA FACULTAD DE CS. EXACTAS Y NATURALES
Citación
Ramhorst, Rosanna Elizabeth; Perez Leiros, Claudia; Mor, Gil; Agüero, Mariana; Paparini, Daniel Esteban; Grasso, Esteban Nicolas; et al.; VIP contribution to the decidualization program: regulatory T cell recruitment; BioScientifica; Journal of Endocrinology; 221; 4-2014; 121-131
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