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Artículo

Neutrophils exhibit differential requirements for homing molecules in their lymphatic and blood trafficking into draining lymph nodes

Gorlino, Carolina VirginiaIcon ; Ranocchia, Romina PaolaIcon ; Harman, María FlorenciaIcon ; García, Iris AlejandraIcon ; Crespo, Maria InesIcon ; Moron, Victor GabrielIcon ; Maletto, Belkys Angélica; Pistoresi, Maria CristinaIcon
Fecha de publicación: 07/2014
Editorial: American Association of Immunologists
Revista: Journal of Immunology
ISSN: 0022-1767
Idioma: Inglés
Tipo de recurso: Artículo publicado
Clasificación temática:
Otras Ciencias de la Salud

Resumen

Although much is described about the molecules involved in neutrophil migration from circulation into tissues, less is known about the molecular mechanisms that regulate neutrophil entry into lymph nodes (LNs) draining a local inflammatory site. In this study, we investigated neutrophil migration toward LNs in a context of inflammation induced by immunization of BALB/c mice with OVA emulsified in CFA. We demonstrated that neutrophils can enter LNs of OVA/CFA-immunized mice not only via lymphatic vessels but also from blood, across high endothelial venules. By adoptive transfer experiments, we showed that this influx was dependent on an inflammatory-state condition and previous neutrophil stimulation with OVA/anti-OVA immune complexes. Importantly, we have demonstrated that, in the migratory pattern to LNs, neutrophils used L-selectin and P-selectin glycoprotein ligand-1, macrophage-1 Ag and LFA-1 integrins, and CXCR4 to get access across high endothelial venules, whereas macrophage-1 Ag, LFA-1, and CXCR4 were involved in their trafficking through afferent lymphatics. Strikingly, we found that stimulation with immune complexes significantly upregulated the expression of sphingosine-1-phosphate receptor 4 on neutrophils, and that treatment with the sphingosine-1-phosphate agonist FTY720 altered neutrophil LN-homing ability. These findings summarized in this article disclose the molecular pattern that controls neutrophil recruitment to LNs.
Palabras clave: Neutrophils , Cell Trafficking , Lymph Nodes
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info:eu-repo/semantics/openAccess Excepto donde se diga explícitamente, este item se publica bajo la siguiente descripción: Creative Commons Attribution-NonCommercial-ShareAlike 2.5 Unported (CC BY-NC-SA 2.5)
Identificadores
URI: http://hdl.handle.net/11336/32157
URL: http://www.jimmunol.org/content/193/4/1966.long
URL: http://www.jimmunol.org/content/193/4/1966
Colecciones
Articulos(CIBICI)
Articulos de CENTRO DE INV.EN BIOQUI.CLINICA E INMUNOLOGIA
Articulos(IMIBIO-SL)
Articulos de INST. MULTIDICIPLINARIO DE INV. BIO. DE SAN LUIS
Citación
Gorlino, Carolina Virginia; Ranocchia, Romina Paola; Harman, María Florencia; García, Iris Alejandra; Crespo, Maria Ines; et al.; Neutrophils exhibit differential requirements for homing molecules in their lymphatic and blood trafficking into draining lymph nodes; American Association of Immunologists; Journal of Immunology; 193; 4; 7-2014; 1966-1974
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