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dc.contributor.author Suárez, Cecilia Ana
dc.contributor.author Diaz, Graciela Susana
dc.contributor.author Gonzalez Iglesias, Arturo
dc.contributor.author Vela, Jorge
dc.contributor.author Mladovan, Alejandro
dc.contributor.author Baldi, Alberto
dc.contributor.author Becu, Damasia
dc.date.available 2017-12-28T20:58:20Z
dc.date.issued 2003-09-01
dc.identifier.citation Becu, Damasia; Baldi, Alberto; Mladovan, Alejandro; Vela, Jorge; Gonzalez Iglesias, Arturo; Diaz, Graciela Susana; et al.; Angiotensin II phosphorylation of extracellular signal regulated kinases in rat anterior pituitary cells; American Physiological Society; American Journal Of Physiology-endocrinology And Metabolism; 285; 3; 1-9-2003; E645-E653
dc.identifier.issn 0193-1849
dc.identifier.uri http://hdl.handle.net/11336/31899
dc.description.abstract We studied the effects of ANG II on extracellular signal-regulated kinase (ERK)1/2 phosphorylation in rat pituitary cells. ANG II increased ERK phosphorylation in a time- and concentration-dependent way. Maximum effect was obtained at 5 min at a concentration of 10-100 nM. The effect of 100 nM ANG II was blocked by the AT1 antagonist DUP-753, by the phospholipase C (PLC) inhibitor U-73122, and by the MAPK kinase (MEK) antagonist PD-98059. The ANG II-induced increase in phosphorylated (p)ERK was insensitive to pertussis toxin blockade and PKC depletion or inhibition. The effect was also abrogated by chelating intracellular calcium with BAPTA-AM or TMB-8 by depleting intracellular calcium stores with a 30-min pretreatment with EGTA and by pretreatment with herbimycin A and PP1, two c-Src tyrosine kinase inhibitors. It was attenuated by AG-1478, an inhibitor of epidermal growth factor receptor (EGFR) activation. Therefore, in the rat pituitary, the increase of pERK is a Gq- and PLC-dependent process, which involves an increase in intracellular calcium and activation of a c-Src tyrosine kinase, transactivation of the EGFR, and the activation of MEK. Finally, the response of ERK activation by ANG II is altered in hyperplastic pituitary cells, in which calcium mobilization evoked by ANG II is also modified.
dc.format application/pdf
dc.language.iso eng
dc.publisher American Physiological Society
dc.rights info:eu-repo/semantics/restrictedAccess
dc.rights.uri https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.subject ANGIOTENSIN
dc.subject CALCIUM
dc.subject HYPERPLASIA
dc.subject PITUITARY GLAND
dc.subject PROTEIN KINASE
dc.subject.classification Bioquímica y Biología Molecular
dc.subject.classification Medicina Básica
dc.subject.classification CIENCIAS MÉDICAS Y DE LA SALUD
dc.title Angiotensin II phosphorylation of extracellular signal regulated kinases in rat anterior pituitary cells
dc.type info:eu-repo/semantics/article
dc.type info:ar-repo/semantics/artículo
dc.type info:eu-repo/semantics/publishedVersion
dc.date.updated 2017-12-28T13:40:52Z
dc.identifier.eissn 1522-1555
dc.journal.volume 285
dc.journal.number 3
dc.journal.pagination E645-E653
dc.journal.pais Estados Unidos
dc.journal.ciudad Bethesda
dc.description.fil Fil: Suárez, Cecilia Ana. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biología y Medicina Experimental. Fundación de Instituto de Biología y Medicina Experimental. Instituto de Biología y Medicina Experimental; Argentina
dc.description.fil Fil: Diaz, Graciela Susana. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biología y Medicina Experimental. Fundación de Instituto de Biología y Medicina Experimental. Instituto de Biología y Medicina Experimental; Argentina
dc.description.fil Fil: Gonzalez Iglesias, Arturo. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biología y Medicina Experimental. Fundación de Instituto de Biología y Medicina Experimental. Instituto de Biología y Medicina Experimental; Argentina
dc.description.fil Fil: Vela, Jorge. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biología y Medicina Experimental. Fundación de Instituto de Biología y Medicina Experimental. Instituto de Biología y Medicina Experimental; Argentina
dc.description.fil Fil: Mladovan, Alejandro. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biología y Medicina Experimental. Fundación de Instituto de Biología y Medicina Experimental. Instituto de Biología y Medicina Experimental; Argentina
dc.description.fil Fil: Baldi, Alberto. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biología y Medicina Experimental. Fundación de Instituto de Biología y Medicina Experimental. Instituto de Biología y Medicina Experimental; Argentina
dc.description.fil Fil: Becu, Damasia. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biología y Medicina Experimental. Fundación de Instituto de Biología y Medicina Experimental. Instituto de Biología y Medicina Experimental; Argentina
dc.journal.title American Journal Of Physiology-endocrinology And Metabolism
dc.relation.alternativeid info:eu-repo/semantics/altIdentifier/url/http://www.physiology.org/doi/full/10.1152/ajpendo.00015.2003
dc.relation.alternativeid info:eu-repo/semantics/altIdentifier/doi/http://dx.doi.org/10.1152/ajpendo.00015.2003
dc.relation.alternativeid info:eu-repo/semantics/altIdentifier/pmid/12759218
dc.conicet.fuente individual


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