Artículo
A novel regulator of human villous trophoblast fusion: the Krüppel-like factor 6
Racca, Ana Cristina
; Ridano, Magali Evelin
; Camolotto, Soledad Andrea
; Genti de Raimondi, Susana
; Panzetta-Dutari, Graciela Maria del Valle
Fecha de publicación:
12/2014
Editorial:
Oxford University Press
Revista:
Molecular Human Reproduction
ISSN:
1360-9947
Idioma:
Inglés
Tipo de recurso:
Artículo publicado
Clasificación temática:
Resumen
Cell–cell fusion is an essential event during life. Throughout human pregnancy, the syncytiotrophoblast (STB) layer of the placenta is formed by continuous fusion of the underlying villous cytotrophoblasts, thus maintaining placental functionality. Defects in this process are associated with pathologies like pre-eclampsia and intrauterine growth restriction. Krüppel-like factor 6 (KLF6) is a transcription factor highly expressed in human and murine placenta. However, KLF6 functions in trophoblast cells remain largely unexplored. The aim of this work was to address the role of KLF6 during STB formation. KLF6 knockdown through small interfering RNA experiments hindered cell–cell fusion revealed by immunofluorescence microscopy in human primary villous cytotrophoblast as well as in the human placental-derived BeWo cell line. Furthermore, KLF6 silencing led to a decrease in the expression of the fusogenic protein Syncytin-1 and the cell cycle regulator p21Cip1/Waf1measured by quantitative RT-PCR and western blot assays. On the contrary, transcript levels of genes that encode for proteins involved in STB formation such as Syncytin-1, Syncytin-2, Connexin-43 and Zonula Occludens-1 increased when KLF6 was overexpressed in differentiating villous cytotrophoblasts and in non-fusing placental-derived JEG-3 cells. Interestingly, the expression of two trophoblast biochemical differentiation markers, βhCG and PSG3, were not reduced after KLF6 silencing in differentiating trophoblast cells. Present results support the notion that KLF6 is a relevant participant in cytotrophoblast fusion.
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Articulos(CIBICI)
Articulos de CENTRO DE INV.EN BIOQUI.CLINICA E INMUNOLOGIA
Articulos de CENTRO DE INV.EN BIOQUI.CLINICA E INMUNOLOGIA
Citación
Racca, Ana Cristina; Ridano, Magali Evelin; Camolotto, Soledad Andrea; Genti de Raimondi, Susana; Panzetta-Dutari, Graciela Maria del Valle; A novel regulator of human villous trophoblast fusion: the Krüppel-like factor 6; Oxford University Press; Molecular Human Reproduction; 21; 4; 12-2014; 347-358
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