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Artículo

Mapping the distribution of conformational information throughout a protein sequence

Gebhard, Leopoldo GermanIcon ; Risso, Valeria AlejandraIcon ; Santos, JavierIcon ; Ferreyra, Raul GabrielIcon ; Noguera, Martín EzequielIcon ; Ermacora, Mario RobertoIcon
Fecha de publicación: 02/2006
Editorial: Elsevier
Revista: Journal Of Molecular Biology
ISSN: 0022-2836
Idioma: Inglés
Tipo de recurso: Artículo publicado

Resumen

The three-dimensional structure of protein is encoded in the sequence, but many amino acid residues carry no essential conformational information, and the identity of those that are structure-determining is elusive. By circular permutation and terminal deletion, we produced and purified 25 Bacillus licheniformis β-lactamase (ESBL) variants that lack 5–21 contiguous residues each, and collectively have 82% of the sequence and 92% of the non-local atom–atom contacts eliminated. Circular dichroism and size-exclusion chromatography showed that most of the variants form conformationally heterogeneous mixtures, but by measuring catalytic constants, we found that all populate, to a greater or lesser extent, conformations with the essential features of the native fold. This suggests that no segment of the ESBL sequence is essential to the structure as a whole, which is congruent with the notion that local information and modular organization can impart most of the tertiary fold specificity and cooperativity.
Palabras clave: Protein Folding , Beta-Lactamase , Conformational Information , Sequence Patterns , Folding Code , Folding Units
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info:eu-repo/semantics/restrictedAccess Excepto donde se diga explícitamente, este item se publica bajo la siguiente descripción: Creative Commons Attribution-NonCommercial-ShareAlike 2.5 Unported (CC BY-NC-SA 2.5)
Identificadores
URI: http://hdl.handle.net/11336/31748
URL: https://www.sciencedirect.com/science/article/pii/S0022283606001495
DOI: http://dx.doi.org/10.1016/j.jmb.2006.01.095
Colecciones
Articulos(IMBICE) [287]
Articulos de INST.MULTIDISCIPL.DE BIOLOGIA CELULAR (I)
Articulos(IIBBA) [918]
Articulos de INST.DE INVEST.BIOQUIMICAS DE BS.AS(I)
Articulos(IQUIFIB) [444]
Articulos de INST.DE QUIMICA Y FISICO-QUIMICA BIOLOGICAS "PROF. ALEJANDRO C. PALADINI"
Articulos(CCT - LA PLATA) [5590]
Articulos de CTRO.CIENTIFICO TECNOL.CONICET - LA PLATA
Articulos(SEDE CENTRAL) [20919]
Articulos de SEDE CENTRAL
Citación
Ermacora, Mario Roberto; Noguera, Martín Ezequiel; Ferreyra, Raul Gabriel; Santos, Javier; Risso, Valeria Alejandra; Gebhard, Leopoldo German; et al.; Mapping the distribution of conformational information throughout a protein sequence; Elsevier; Journal Of Molecular Biology; 358; 1; 2-2006; 280-288
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