Artículo
Mapping the distribution of conformational information throughout a protein sequence
Gebhard, Leopoldo German
; Risso, Valeria Alejandra
; Santos, Javier
; Ferreyra, Raul Gabriel
; Noguera, Martín Ezequiel
; Ermacora, Mario Roberto






Fecha de publicación:
02/2006
Editorial:
Elsevier
Revista:
Journal Of Molecular Biology
ISSN:
0022-2836
Idioma:
Inglés
Tipo de recurso:
Artículo publicado
Resumen
The three-dimensional structure of protein is encoded in the sequence, but many amino acid residues carry no essential conformational information, and the identity of those that are structure-determining is elusive. By circular permutation and terminal deletion, we produced and purified 25 Bacillus licheniformis β-lactamase (ESBL) variants that lack 5–21 contiguous residues each, and collectively have 82% of the sequence and 92% of the non-local atom–atom contacts eliminated. Circular dichroism and size-exclusion chromatography showed that most of the variants form conformationally heterogeneous mixtures, but by measuring catalytic constants, we found that all populate, to a greater or lesser extent, conformations with the essential features of the native fold. This suggests that no segment of the ESBL sequence is essential to the structure as a whole, which is congruent with the notion that local information and modular organization can impart most of the tertiary fold specificity and cooperativity.
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Articulos(IMBICE) [287]
Articulos de INST.MULTIDISCIPL.DE BIOLOGIA CELULAR (I)
Articulos de INST.MULTIDISCIPL.DE BIOLOGIA CELULAR (I)
Articulos(IIBBA) [918]
Articulos de INST.DE INVEST.BIOQUIMICAS DE BS.AS(I)
Articulos de INST.DE INVEST.BIOQUIMICAS DE BS.AS(I)
Articulos(IQUIFIB) [444]
Articulos de INST.DE QUIMICA Y FISICO-QUIMICA BIOLOGICAS "PROF. ALEJANDRO C. PALADINI"
Articulos de INST.DE QUIMICA Y FISICO-QUIMICA BIOLOGICAS "PROF. ALEJANDRO C. PALADINI"
Articulos(CCT - LA PLATA) [5590]
Articulos de CTRO.CIENTIFICO TECNOL.CONICET - LA PLATA
Articulos de CTRO.CIENTIFICO TECNOL.CONICET - LA PLATA
Articulos(SEDE CENTRAL) [20919]
Articulos de SEDE CENTRAL
Articulos de SEDE CENTRAL
Citación
Ermacora, Mario Roberto; Noguera, Martín Ezequiel; Ferreyra, Raul Gabriel; Santos, Javier; Risso, Valeria Alejandra; Gebhard, Leopoldo German; et al.; Mapping the distribution of conformational information throughout a protein sequence; Elsevier; Journal Of Molecular Biology; 358; 1; 2-2006; 280-288
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