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dc.contributor.author
Zacarías, María Florencia  
dc.contributor.author
Reinheimer, Jorge Alberto  
dc.contributor.author
Forzani, Liliana Maria  
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Grangette, Corinne  
dc.contributor.author
Vinderola, Celso Gabriel  
dc.date.available
2017-12-27T18:09:03Z  
dc.date.issued
2014-03  
dc.identifier.citation
Vinderola, Celso Gabriel; Reinheimer, Jorge Alberto; Grangette, Corinne; Forzani, Liliana Maria; Zacarías, María Florencia; Mortality and translocation assay to study the protective capacity of Bifidobacterium lactis INL1 against Salmonella Typhimurium infection in mice; Wageningen Academic Publishers; Beneficial Microbes; 5; 4; 3-2014; 427-436  
dc.identifier.issn
1876-2883  
dc.identifier.uri
http://hdl.handle.net/11336/31677  
dc.description.abstract
The mouse has been largely used for the study of the protective capacity of probiotics against intestinal infections caused by Salmonella. In this work we aimed at comparing the mortality and translocation assay for the study of the protective capacity of the human breast milk-derived strain Bifidobacterium animalis subsp. lactis INL1 on a model of gut infection by Salmonella enterica subsp. enterica serovar Typhimurium. Different doses of S. Typhimurium FUNED and B. animalis subsp. lactis INL 1 were administered to Balb/c mice in a mortality or a translocation assay. The survival of the control group in the mortality assay resulted to be variable along experiments, and then we preferred to use a translocation assay where the preventive administration of 109 cfu of bifidobacteria/mouse for 10 consecutive days significantly reduced the number of infected animals and the levels of translocation to liver and spleen, with enhanced secretory immunoglobulin A and interleukin 10 production in the small and large intestine, respectively. Ten days of B. animalis subsp. lactis strain INL1 administration to mice significantly reduced both the incidence and the severity of Salmonella infection in a mouse model of translocation. This work provided the first evidence that a translocation assay, compared to a mortality assay, could be more useful to study the protective capacity of probiotics against Salmonella infection, as more information can be obtained from mice and less suffering is conferred to animals due to the fact that the mortality assay is shorter than the latter. These facts are in line with the guidelines of animal research recently established by the National Centre for the Replacement, Refinement & Reduction of Animals in Research.  
dc.format
application/pdf  
dc.language.iso
eng  
dc.publisher
Wageningen Academic Publishers  
dc.rights
info:eu-repo/semantics/openAccess  
dc.rights.uri
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/  
dc.subject
Probiotic  
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Mousse Model  
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Salmonella  
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Infection  
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Bifidobacteria  
dc.subject.classification
Alimentos y Bebidas  
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Otras Ingenierías y Tecnologías  
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INGENIERÍAS Y TECNOLOGÍAS  
dc.title
Mortality and translocation assay to study the protective capacity of Bifidobacterium lactis INL1 against Salmonella Typhimurium infection in mice  
dc.type
info:eu-repo/semantics/article  
dc.type
info:ar-repo/semantics/artículo  
dc.type
info:eu-repo/semantics/publishedVersion  
dc.date.updated
2017-12-26T19:51:31Z  
dc.identifier.eissn
1876-2891  
dc.journal.volume
5  
dc.journal.number
4  
dc.journal.pagination
427-436  
dc.journal.pais
Países Bajos  
dc.description.fil
Fil: Zacarías, María Florencia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe. Instituto de Lactología Industrial. Universidad Nacional del Litoral. Facultad de Ingeniería Química. Instituto de Lactología Industrial; Argentina  
dc.description.fil
Fil: Reinheimer, Jorge Alberto. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe. Instituto de Lactología Industrial. Universidad Nacional del Litoral. Facultad de Ingeniería Química. Instituto de Lactología Industrial; Argentina  
dc.description.fil
Fil: Forzani, Liliana Maria. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe. Instituto de Matemática Aplicada del Litoral. Universidad Nacional del Litoral. Instituto de Matemática Aplicada del Litoral; Argentina. Universidad Nacional del Litoral. Facultad de Ingeniería Química. Departamento de Matemáticas; Argentina  
dc.description.fil
Fil: Grangette, Corinne. Instituto Pasteur; Francia. Centre National de la Recherche Scientifique; Francia  
dc.description.fil
Fil: Vinderola, Celso Gabriel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe. Instituto de Lactología Industrial. Universidad Nacional del Litoral. Facultad de Ingeniería Química. Instituto de Lactología Industrial; Argentina  
dc.journal.title
Beneficial Microbes  
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/url/http://www.wageningenacademic.com/doi/abs/10.3920/BM2013.0086?url_ver=Z39.88-2003&rfr_id=ori%3Arid%3Acrossref.org&rfr_dat=cr_pub%3Dpubmed&  
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/doi/http://dx.doi.org/10.3920/BM2013.0086