Artículo
Identification of Giardia lamblia DHHC Proteins and the Role of Protein S-palmitoylation in the Encystation Process
Merino, Maria Cecilia
; Zamponi, Nahuel
; Vranych, Cecilia Verónica
; Touz, Maria Carolina
; Ropolo, Andrea Silvana
Fecha de publicación:
07/2014
Editorial:
Public Library of Science
Revista:
Neglected Tropical Diseases
ISSN:
1935-2735
Idioma:
Inglés
Tipo de recurso:
Artículo publicado
Clasificación temática:
Resumen
Protein S-palmitoylation, a hydrophobic post-translational modification, is performed by protein acyltransferases that have a common DHHC Cys-rich domain (DHHC proteins), and provides a regulatory switch for protein membrane association. In this work, we analyzed the presence of DHHC proteins in the protozoa parasite Giardia lamblia and the function of the reversible S-palmitoylation of proteins during parasite differentiation into cyst. Two specific events were observed: encysting cells displayed a larger amount of palmitoylated proteins, and parasites treated with palmitoylation inhibitors produced a reduced number of mature cysts. With bioinformatics tools, we found nine DHHC proteins, potential protein acyltransferases, in the Giardia proteome. These proteins displayed a conserved structure when compared to different organisms and are distributed in different monophyletic clades. Although all Giardia DHHC proteins were found to be present in trophozoites and encysting cells, these proteins showed a different intracellular localization in trophozoites and seemed to be differently involved in the encystation process when they were overexpressed. dhhc transgenic parasites showed a different pattern of cyst wall protein expression and yielded different amounts of mature cysts when they were induced to encyst. Our findings disclosed some important issues regarding the role of DHHC proteins and palmitoylation during Giardia encystation.
Palabras clave:
Palmitoylation
,
Transferase
,
Dhhc Motif
,
Giardia
Archivos asociados
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Identificadores
Colecciones
Articulos(INIMEC - CONICET)
Articulos de INSTITUTO DE INV. MEDICAS MERCEDES Y MARTIN FERREYRA
Articulos de INSTITUTO DE INV. MEDICAS MERCEDES Y MARTIN FERREYRA
Citación
Ropolo, Andrea Silvana; Vranych, Cecilia Verónica; Zamponi, Nahuel; Merino, Maria Cecilia; Touz, Maria Carolina; Identification of Giardia lamblia DHHC Proteins and the Role of Protein S-palmitoylation in the Encystation Process; Public Library of Science; Neglected Tropical Diseases; 8; 7; 7-2014; e2997-1-17
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