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dc.contributor.author
Raimunda, Daniel Cesar
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dc.contributor.author
Elso, Graciela Raquel
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dc.date.available
2017-12-27T13:46:23Z
dc.date.issued
2014-12
dc.identifier.citation
Elso, Graciela Raquel; Raimunda, Daniel Cesar; Functional characterization of the CDF transporter SMc02724 (SmYiiP) in Sinorhizobium meliloti: Roles in manganese homeostasis and nodulation; Elsevier Science; Biochimica et Biophysica Acta - Biomembranes; 1838; 12-2014; 3203-3211
dc.identifier.issn
0005-2736
dc.identifier.uri
http://hdl.handle.net/11336/31627
dc.description.abstract
In bacteria, membrane transporters of the cation diffusion facilitator (CDF) family participate in Zn2+,Fe2+,Mn2+,Co2+and Ni2+homeostasis. The functional role during infection processes for several members has been shown tobe linked to the specificity of transport.Sinorhizobium melilotihas two homologous CDF genes with unknowntransport specificity. Here we evaluate the role played by the CDF SMc02724 (SmYiiP). The deletion mutant strainof SmYiiP (ΔsmyiiP) showed reduced in vitro growthfitness only in the presence of Mn2+.IncubationofΔsmyiiPand WT cells with sub-lethal Mn2+concentrations resulted in a 2-fold increase of the metal only in the mutantstrain. Normal levels of resistance to Mn2+were attained by complementation with the gene SMc02724 underregulation of its endogenous promoter. In vitro, liposomes with incorporated heterologously expressed pure pro-tein accumulated several transition metals. However, only the transport rate of Mn2+was increased by imposing atransmembrane H+gradient. Nodulation assays in alfalfa plants showed that the strainΔsmyiiPinduced a lowernumber of nodules than in plants infected with the WT strain. Our results indicate that Mn2+homeostasis inS. melilotiis required for full infection capacity, or nodule function, and that the specificity of transport in vivo ofSmYiiP is narrowed down to Mn2+by a mechanism involving the proton motive force.
dc.format
application/pdf
dc.language.iso
eng
dc.publisher
Elsevier Science
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dc.rights
info:eu-repo/semantics/openAccess
dc.rights.uri
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.subject
Cation Diffusion Facilitator
dc.subject
Manganese Homeostasis
dc.subject
Manganese Efflux
dc.subject
Sinorhizobium Meliloti
dc.subject.classification
Otras Ciencias Biológicas
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dc.subject.classification
Ciencias Biológicas
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dc.subject.classification
CIENCIAS NATURALES Y EXACTAS
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dc.title
Functional characterization of the CDF transporter SMc02724 (SmYiiP) in Sinorhizobium meliloti: Roles in manganese homeostasis and nodulation
dc.type
info:eu-repo/semantics/article
dc.type
info:ar-repo/semantics/artículo
dc.type
info:eu-repo/semantics/publishedVersion
dc.date.updated
2017-12-26T20:39:15Z
dc.journal.volume
1838
dc.journal.pagination
3203-3211
dc.journal.pais
Países Bajos
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dc.journal.ciudad
Amsterdam
dc.description.fil
Fil: Raimunda, Daniel Cesar. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Investigación Médica Mercedes y Martín Ferreyra. Universidad Nacional de Córdoba. Instituto de Investigación Médica Mercedes y Martín Ferreyra; Argentina
dc.description.fil
Fil: Elso, Graciela Raquel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Investigación Médica Mercedes y Martín Ferreyra. Universidad Nacional de Córdoba. Instituto de Investigación Médica Mercedes y Martín Ferreyra; Argentina
dc.journal.title
Biochimica et Biophysica Acta - Biomembranes
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dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/doi/http://dx.doi.org/10.1016/j.bbamem.2014.09.005
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/url/http://www.sciencedirect.com/science/article/pii/S0005273614003204
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