Show simple item record

dc.contributor.author Gonzalez Deniselle, Maria Claudia
dc.contributor.author Garay, Laura Ines
dc.contributor.author Gonzalez, Susana Laura
dc.contributor.author Guennoun, Rachida
dc.contributor.author Schumacher, Michael
dc.contributor.author de Nicola, Alejandro Federico
dc.date.available 2017-12-23T19:44:58Z
dc.date.issued 2005-10
dc.identifier.citation de Nicola, Alejandro Federico; Schumacher, Michael; Guennoun, Rachida; Gonzalez, Susana Laura; Garay, Laura Ines; Gonzalez Deniselle, Maria Claudia; et al.; Progesterone restores retrograde labelling of cervical motoneurons in Wobbler mouse motoneuron disease; Academic Press Inc Elsevier Science; Experimental Neurology; 195; 2; 10-2005; 518-523
dc.identifier.issn 0014-4886
dc.identifier.uri http://hdl.handle.net/11336/31467
dc.description.abstract The Wobbler mouse, a mutant characterized by motoneuron degeneration in the cervical spinal cord, has been used to test the efficacy of novel treatments for human motoneuron diseases (HMD). Previous reports have shown that slow axonal transport is impaired in Wobblers and other models of HMD. Since progesterone (PROG) corrects some morphological, molecular, and functional abnormalities of Wobbler mice, we studied if steroid exposure for 8 weeks restored retrograde axonal transport by measuring motoneuron labeling after injection of fluorogold into the limb muscles. The dye was injected into forelimb biceps bracchii and flexor or into the rearlimb gastrocnemius muscles; 6 days later, the number of fluorescent motoneurons and the total number of cresyl violet stained motoneurons were counted in the cervical (C5-T1) or lumbar (L3-L5) spinal cord regions. A pronounced reduction (- 42.2%) of the percent of fluorescent motoneurons in Wobbler mice cervical cord was noted, which was significantly corrected after PROG treatment. In contrast, labeling of lumbar motoneurons was not reduced in Wobbler mice and was not affected by PROG treatment. In no case PROG showed an effect in control mice. Concomitantly, PROG slightly but significantly increased biceps weight of Wobbler mice. Behaviorally, PROG-treated Wobblers performed better on a motor test (hanging time from a horizontal rope) compared to untreated counterparts. We postulate a dual role for PROG in the Wobbler mouse, in part by prevention of motoneuron degeneration and also by enhancement of axonal transport. The latter mechanism could improve the traffic of neurotrophic factors from the forelimb muscles into the ailing motoneurons, improving neuromuscular function in this murine model of HMD.
dc.format application/pdf
dc.language.iso eng
dc.publisher Academic Press Inc Elsevier Science
dc.rights info:eu-repo/semantics/restrictedAccess
dc.rights.uri https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.subject WOBBLER
dc.subject AMYOTROPHIC LATERAL SCLEROSIS
dc.subject NEURODEGENERATION
dc.subject PROGESTERONE
dc.subject.classification Neurociencias
dc.subject.classification Medicina Básica
dc.subject.classification CIENCIAS MÉDICAS Y DE LA SALUD
dc.title Progesterone restores retrograde labelling of cervical motoneurons in Wobbler mouse motoneuron disease
dc.type info:eu-repo/semantics/article
dc.type info:ar-repo/semantics/artículo
dc.type info:eu-repo/semantics/publishedVersion
dc.date.updated 2017-12-19T18:38:04Z
dc.identifier.eissn 1090-2430
dc.journal.volume 195
dc.journal.number 2
dc.journal.pagination 518-523
dc.journal.pais Estados Unidos
dc.journal.ciudad Nueva York
dc.description.fil Fil: Gonzalez Deniselle, Maria Claudia. Universidad de Buenos Aires. Facultad de Medicina; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biología y Medicina Experimental. Fundación de Instituto de Biología y Medicina Experimental. Instituto de Biología y Medicina Experimental; Argentina
dc.description.fil Fil: Garay, Laura Ines. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biología y Medicina Experimental. Fundación de Instituto de Biología y Medicina Experimental. Instituto de Biología y Medicina Experimental; Argentina. Universidad de Buenos Aires. Facultad de Medicina. Departamento de Bioquímica Humana; Argentina
dc.description.fil Fil: Gonzalez, Susana Laura. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biología y Medicina Experimental. Fundación de Instituto de Biología y Medicina Experimental. Instituto de Biología y Medicina Experimental; Argentina. Universidad de Buenos Aires. Facultad de Medicina. Departamento de Bioquímica Humana; Argentina
dc.description.fil Fil: Guennoun, Rachida. Inserm; Francia
dc.description.fil Fil: Schumacher, Michael. Inserm; Francia
dc.description.fil Fil: de Nicola, Alejandro Federico. Universidad de Buenos Aires. Facultad de Medicina; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biología y Medicina Experimental. Fundación de Instituto de Biología y Medicina Experimental. Instituto de Biología y Medicina Experimental; Argentina. Instituto Universidad de la Fundación ; Argentina
dc.journal.title Experimental Neurology
dc.relation.alternativeid info:eu-repo/semantics/altIdentifier/url/http://www.sciencedirect.com/science/article/pii/S0014488605002232?via%3Dihub
dc.relation.alternativeid info:eu-repo/semantics/altIdentifier/doi/http://dx.doi.org/10.1016/j.expneurol.2005.06.015
dc.relation.alternativeid info:eu-repo/semantics/altIdentifier/pmid/16095593
dc.conicet.fuente unificacion


Archivos asociados

Icon
Blocked Acceso no disponible

This item appears in the following Collection(s)

  • Articulos(IBYME) [1050]
    Articulos de INST.DE BIOLOGIA Y MEDICINA EXPERIMENTAL (I)

Show simple item record

info:eu-repo/semantics/restrictedAccess Excepto donde se diga explícitamente, este item se publica bajo la siguiente descripción: Creative Commons Attribution-NonCommercial-ShareAlike 2.5 Unported (CC BY-NC-SA 2.5)