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Artículo

Progestin activation of nongenomic pathways via cross talk of progesterone receptor with estrogen receptor beta induces proliferation of endometrial stromal cells

Vallejo, GriseldaIcon ; Ballare, Cecilia; Barañao, Jose Lino SalvadorIcon ; Beato, Miguel; Saragueta, Patricia EstherIcon
Fecha de publicación: 12/2005
Editorial: Endocrine Society
Revista: Molecular Endocrinology
ISSN: 0888-8809
e-ISSN: 1944-9917
Idioma: Inglés
Tipo de recurso: Artículo publicado
Clasificación temática:
Fisiología; Biología Reproductiva

Resumen

Uterine decidualization is characterized by stromal cell proliferation and differentiation, which are controlled by ovarian hormones estradiol and progesterone. Here we report that the proliferative response of UIII rat uterine stromal cells to a short treatment with progestins requires active progesterone receptor (PR) and estrogen receptor beta (ERbeta) as well as a rapid and transient activation of Erk1-2 and Akt signaling. The optimal R5020 concentration for the proliferative response as well as for activation of the signaling cascades was between 10 and 100 pm. UIII cells are negative for ERalpha and have low levels of ERbeta and PR located mainly in the cytoplasm. Upon progestin treatment PR translocated to the cell nucleus where it colocalized with activated Erk1-2. Neither progestins nor estradiol transactivated the corresponding transfected reporter genes, suggesting that endogenous PR and ERbeta are transcriptionally incompetent. A fraction of endogenous PR and ERbeta form a complex as demonstrated by coimmunoprecipitation. Taken together, our results suggest that the proliferative response of uterine stromal cells to picomolar concentrations of progestins does not require direct transcriptional effects and is mediated by activation of the Erk1-2 and Akt signaling pathways via cross talk between PR and ERbeta.
Palabras clave: Cell Proliferation , Cytoplasm , Endometrium , Estrogen Receptor , Mitogen-Activated Protein Kinase 1 , Mitogen-Activated Protein Kinase 3 , Signal Transduction , Stromal Cells , Transcriptional Activation
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info:eu-repo/semantics/openAccess Excepto donde se diga explícitamente, este item se publica bajo la siguiente descripción: Creative Commons Attribution-NonCommercial-ShareAlike 2.5 Unported (CC BY-NC-SA 2.5)
Identificadores
URI: http://hdl.handle.net/11336/31464
DOI: http://dx.doi.org/10.1210/me.2005-0016
Colecciones
Articulos(IBYME)
Articulos de INST.DE BIOLOGIA Y MEDICINA EXPERIMENTAL (I)
Citación
Vallejo, Griselda; Ballare, Cecilia; Barañao, Jose Lino Salvador; Beato, Miguel; Saragueta, Patricia Esther; Progestin activation of nongenomic pathways via cross talk of progesterone receptor with estrogen receptor beta induces proliferation of endometrial stromal cells; Endocrine Society; Molecular Endocrinology; 19; 12; 12-2005; 3023-3037
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