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dc.contributor.author
Correa Garcia, Susana Raquel
dc.contributor.author
Bermudez Moretti, Mariana
dc.contributor.author
Travo, Adrian
dc.contributor.author
Deleris, Gerard
dc.contributor.author
Forfar, Isabelle
dc.date.available
2017-12-21T20:05:29Z
dc.date.issued
2014-02
dc.identifier.citation
Correa Garcia, Susana Raquel; Bermudez Moretti, Mariana; Travo, Adrian; Deleris, Gerard; Forfar, Isabelle; FTIR spectroscopic metabolome analysis of lyophilized and fresh Saccharomyces cerevisiae yeast cells; Royal Society of Chemistry; Analytical Methods; 6; 6; 2-2014; 1855-1861
dc.identifier.issn
1759-9660
dc.identifier.uri
http://hdl.handle.net/11336/31287
dc.description.abstract
The yeast Saccharomyces cerevisiae is widely used as a biological eukaryotic model and also serves as a production organism in biotechnology. One of the methods used to avoid degradation of the yeast cell content is lyophilization. The use of lyophilized yeast cells has several advantages over fresh ones: samples can be easily transported and/or stored and variations of their metabolomic profiles do not occur during transport or storage. Fourier transform infrared (FTIR) spectroscopy is one of the most emerging approaches in modern biology that permits operation on very small quantities of whole cells without the need for extractions or purifications. This technique is very sensitive and not only allows the discrimination between different cell genotypes but also between different growth conditions. FTIR spectra provide interesting data on the metabolic status of the whole cell. Modern multivariate data processing was applied to analyse live fresh or lyophilized S. cerevisiae cells from different growth media. This study clearly demonstrates that yeast cells coming from an identical biological medium can be used indiscriminately for FTIR analysis whether they are analysed directly as live fresh cells or after lyophilization which is a freeze-drying process. Moreover, FTIR data obtained using lyophilized cells showed less variability.
dc.format
application/pdf
dc.language.iso
eng
dc.publisher
Royal Society of Chemistry
dc.rights
info:eu-repo/semantics/openAccess
dc.rights.uri
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.subject
Yeast
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Ftir
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Freeze Drying
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Pls-Da
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Otras Ciencias Biológicas
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Ciencias Biológicas
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CIENCIAS NATURALES Y EXACTAS
dc.title
FTIR spectroscopic metabolome analysis of lyophilized and fresh Saccharomyces cerevisiae yeast cells
dc.type
info:eu-repo/semantics/article
dc.type
info:ar-repo/semantics/artículo
dc.type
info:eu-repo/semantics/publishedVersion
dc.date.updated
2017-12-12T18:50:15Z
dc.journal.volume
6
dc.journal.number
6
dc.journal.pagination
1855-1861
dc.journal.pais
Reino Unido
dc.description.fil
Fil: Correa Garcia, Susana Raquel. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Instituto de Química Biológica de la Facultad de Ciencias Exactas y Naturales. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Instituto de Química Biológica de la Facultad de Ciencias Exactas y Naturales; Argentina
dc.description.fil
Fil: Bermudez Moretti, Mariana. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Instituto de Química Biológica de la Facultad de Ciencias Exactas y Naturales. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Instituto de Química Biológica de la Facultad de Ciencias Exactas y Naturales; Argentina
dc.description.fil
Fil: Travo, Adrian. Universite de Bordeaux; Francia
dc.description.fil
Fil: Deleris, Gerard. Universite de Bordeaux; Francia
dc.description.fil
Fil: Forfar, Isabelle. Universite de Bordeaux; Francia
dc.journal.title
Analytical Methods
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/doi/http://dx.doi.org/10.1039/c3ay42322k
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/url/http://pubs.rsc.org/en/Content/ArticleLanding/2014/AY/c3ay42322k#!divAbstract
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