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Artículo

Optimization and in vivo toxicity evaluation of G4.5 PAMAM dendrimer-risperidone complexes

Prieto, Maria JimenaIcon ; del Rio Zabala, Nahuel Eduardo; Marotta, Cristian HernánIcon ; Carreño Gutierrez, Hector; Arevalo Arevalo, Rosario; Chiaramoni, Nadia SilviaIcon ; Alonso, Silvia del ValleIcon
Fecha de publicación: 02/2014
Editorial: Public Library of Science
Revista: Plos One
ISSN: 1932-6203
Idioma: Inglés
Tipo de recurso: Artículo publicado
Clasificación temática:
Otras Ciencias Biológicas

Resumen

Risperidone is an approved antipsychotic drug belonging to the chemical class of benzisoxazole. This drug has low solubility in aqueous medium and poor bioavailability due to extensive first-pass metabolism and high protein binding (.90%). Since new strategies to improve efficient treatments are needed, we studied the efficiency of anionic G4.5 PAMAM dendrimers as nanocarriers for this therapeutic drug. To this end, we explored dendrimer-risperidone complexation dependence on<br />solvent concentration, pH and molar relationship. The best dendrimer-risperidone incorporation (46 risperidone molecules per dendrimer) was achieved with a mixture of chloroform:methanol 50:50 v/v solution pH 3. In addition, to explore the possible effects of this complex,in vivo studies were carried out in the zebrafish model. Changes in the development of dopaminergic neurons and motoneurons were studied using tyrosine hydroxylase and calretinin, respectively. Physiological<br />changes were studied through histological sections stained with hematoxylin-eosin to observe possible morphological brain changes. The most significant changes were observed when larvae were treated with free risperidone, and no changes were observed when larvae were treated with the complex.
Palabras clave: G4.5 Pamam , In Vivo Toxicity , Dendrimer-Risperidone Complexes , Zebrafish Morphological Changes
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info:eu-repo/semantics/openAccess Excepto donde se diga explícitamente, este item se publica bajo la siguiente descripción: Creative Commons Attribution 2.5 Unported (CC BY 2.5)
Identificadores
URI: http://hdl.handle.net/11336/31250
DOI: http://dx.doi.org/10.1371/journal.pone.0090393
DOI: https://doi.org/10.1371/journal.pone.0090393
Colecciones
Articulos(CCT - LA PLATA)
Articulos de CTRO.CIENTIFICO TECNOL.CONICET - LA PLATA
Articulos(IMBICE)
Articulos de INST.MULTIDISCIPL.DE BIOLOGIA CELULAR (I)
Citación
Alonso, Silvia del Valle; Chiaramoni, Nadia Silvia; Arevalo Arevalo, Rosario; Carreño Gutierrez, Hector; Marotta, Cristian Hernán; del Rio Zabala, Nahuel Eduardo; et al.; Optimization and in vivo toxicity evaluation of G4.5 PAMAM dendrimer-risperidone complexes; Public Library of Science; Plos One; 9; 2; 2-2014; 1-10; e90393
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