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dc.contributor.author
Su, Bin
dc.contributor.author
Biedma, Marina Elizabeth
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dc.contributor.author
Lederle, Alexandre
dc.contributor.author
Peressin, Maryse
dc.contributor.author
Lambotin, Mélanie
dc.contributor.author
Proust, Alizé
dc.contributor.author
Decoville, Thomas
dc.contributor.author
Schmidt, Sylvie
dc.contributor.author
Laumond, Géraldine
dc.contributor.author
Moog, Christiane
dc.date.available
2017-12-21T18:32:39Z
dc.date.issued
2014-02
dc.identifier.citation
Moog, Christiane; Laumond, Géraldine; Schmidt, Sylvie; Decoville, Thomas; Proust, Alizé; Lambotin, Mélanie; et al.; Dendritic Cell-Lymphocyte Crosstalk Downregulates Host Restriction Factor SAMHD1 and Stimulates HIV-1 Replication in Dendritic Cells; American Society for Microbiology; Journal of Virology; 88; 9; 2-2014; 5109-5121
dc.identifier.issn
0022-538X
dc.identifier.uri
http://hdl.handle.net/11336/31243
dc.description.abstract
Human immunodeficiency virus type 1 (HIV-1) replication in dendritic cells (DCs) is restricted by SAMHD1. This factor is counteracted by the viral protein Vpx; Vpx is found in HIV-2 and simian immunodeficiency virus (SIV) from sooty mangabeys (SIVsm) or from macaques (SIVmac) but is absent from HIV-1. We previously observed that HIV-1 replication in immature DCs is stimulated by cocultivation with primary T and B lymphocytes, suggesting that HIV-1 restriction in DCs may be overcome under coculture conditions. Here, we aimed to decipher the mechanism of SAMHD1-mediated restriction in DC-lymphocyte coculture. We found that coculture with lymphocytes downregulated SAMHD1 expression and was associated with increased HIV-1 replication in DCs. Moreover, in infected DC-T lymphocyte cocultures, DCs acquired maturation status and secreted type 1 interferon (alpha interferon [IFN-α]). The blockade of DC-lymphocyte cross talk by anti-ICAM-1 antibody markedly inhibited the stimulation of HIV-1 replication and prevented the downregulation of SAMHD1 expression in cocultured DCs. These results demonstrate that, in contrast to purified DCs, cross talk with lymphocytes downregulates SAMHD1 expression in DCs, triggering HIV-1 replication and an antiviral immune response. Therefore, HIV-1 replication and immune sensing by DCs should be investigated in more physiologically relevant models of DC/lymphocyte coculture.
dc.format
application/pdf
dc.language.iso
eng
dc.publisher
American Society for Microbiology
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dc.rights
info:eu-repo/semantics/openAccess
dc.rights.uri
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.subject
Samhd-1
dc.subject
Dcs
dc.subject
Hiv-1
dc.subject.classification
Otras Ciencias Biológicas
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dc.subject.classification
Ciencias Biológicas
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dc.subject.classification
CIENCIAS NATURALES Y EXACTAS
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dc.title
Dendritic Cell-Lymphocyte Crosstalk Downregulates Host Restriction Factor SAMHD1 and Stimulates HIV-1 Replication in Dendritic Cells
dc.type
info:eu-repo/semantics/article
dc.type
info:ar-repo/semantics/artículo
dc.type
info:eu-repo/semantics/publishedVersion
dc.date.updated
2017-12-12T18:26:14Z
dc.journal.volume
88
dc.journal.number
9
dc.journal.pagination
5109-5121
dc.journal.pais
Estados Unidos
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dc.journal.ciudad
Washington
dc.description.fil
Fil: Su, Bin. Inserm; Francia
dc.description.fil
Fil: Biedma, Marina Elizabeth. Inserm; Francia. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
dc.description.fil
Fil: Lederle, Alexandre. Inserm; Francia
dc.description.fil
Fil: Peressin, Maryse. Inserm; Francia
dc.description.fil
Fil: Lambotin, Mélanie. Inserm; Francia
dc.description.fil
Fil: Proust, Alizé. Inserm; Francia
dc.description.fil
Fil: Decoville, Thomas. Inserm; Francia
dc.description.fil
Fil: Schmidt, Sylvie. Inserm; Francia
dc.description.fil
Fil: Laumond, Géraldine. Inserm; Francia
dc.description.fil
Fil: Moog, Christiane. Inserm; Francia
dc.journal.title
Journal of Virology
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dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/url/http://jvi.asm.org/content/88/9/5109.full
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/doi/http://dx.doi.org/10.1128/JVI.03057-13
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