Artículo
Development of lentiviral vectors for transient and stable protein overexpression in mammalian cells: A new strategy for recombinant human FVIII (rhFVIII) production
Mufarrege, Eduardo Federico
; Antuña, Sebastián; Marina Echeverrigaray; Kratje, Ricardo Bertoldo
; Prieto, Claudio
Fecha de publicación:
11/2013
Editorial:
Elsevier
Revista:
Protein Expression and Purification
ISSN:
1046-5928
Idioma:
Inglés
Tipo de recurso:
Artículo publicado
Clasificación temática:
Resumen
Background: Recombinant protein overexpression in mammalian cells constitutes a real challenge in therapeutic protein production. Following the discovery of intron functionality in gene expression, various expression vectors that include them in their sequences have been developed. In this study, the main goal was to develop new lentiviral vectors (LVs) carrying different promoter and intron-containing 50UTR (50 untranslated region) combinations and the design of LVs for rhFVIII production in Chinese hamster ovary (CHO) cells.
Results: By combining the human cytomegalovirus (CMV) or the elongation factor 1a (EF-1a) promoters along with different 50UTRs that included leader introns, between 2 and 12-fold increases were reached, when transient and stable expression of the enhanced green fluorescent protein (EGFP) and rhFVIII were analyzed. Also, new LVs provided with promoters and 50UTRs from high expression genes, according to a gene database, were designed. Three of them were shown to be superior to the EF-1a promoter in three widely used cell lines.
Conclusion: In the present work, LVs containing different promoters and 50UTRs were designed. In transient and stable assays some of these constructs have shown higher activity compared with commercial promoters and, therefore, constitute promising candidates for therapeutic protein production.
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Articulos(CCT - SANTA FE)
Articulos de CTRO.CIENTIFICO TECNOL.CONICET - SANTA FE
Articulos de CTRO.CIENTIFICO TECNOL.CONICET - SANTA FE
Citación
Antuña, Sebastián; Mufarrege, Eduardo Federico; Prieto, Claudio; Kratje, Ricardo Bertoldo; Marina Echeverrigaray; Development of lentiviral vectors for transient and stable protein overexpression in mammalian cells: A new strategy for recombinant human FVIII (rhFVIII) production; Elsevier; Protein Expression and Purification; 95; 11-2013; 50-56
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