Artículo
The glycan structure in recombinant human FSH affects endocrine activity and global gene expression in human granulosa cells
Loreti, Rosana Nazareth
; Fresno Rodríguez, Cristóbal
; Barrera Cano, Juan David; Andreone, Luz
; Lira Albarran, Saúl; Fernandez, Elmer Andres
; Larrea, Fernando; Campo, Stella Maris
Fecha de publicación:
05/12/2013
Editorial:
Elsevier
Revista:
Molecular and Cellular Endocrinology
ISSN:
0303-7207
Idioma:
Inglés
Tipo de recurso:
Artículo publicado
Clasificación temática:
Resumen
The aim of this study was to analyse the biological response to different recombinant human FSH (rhFSH) glycosylation variants on the endocrine activity and gene expression at whole-genome scale in human granulosa-like tumor cell line, KGN. The effects of differences in rhFSH sialylation and oligosaccharide complexity were determined on steroid hormone and inhibin production. A microarray approach was used to explore gene expression patterns induced by rhFSH glycosylation variants. Set enrichment analysis revealed that hormone sialylation and oligosaccharide complexity in rhFSH differentially affected the expression of genes involved in essential biological processes and molecular functions of KGN cells. The relevance of rhFSH oligosaccharide structure on steroidogenesis was confirmed assessing gene expression by real time-PCR. The results demonstrate that FSH oligosaccharide structure affects expression of genes encoding proteins, growth factors and hormones essential for granulosa cells function.
Palabras clave:
FSH
,
GENE EXPRESSION
,
GLYCOSYLATION VARIANT
,
HUMAN GRANULOSA CELL
,
INHIBIN
,
STEROID
Archivos asociados
Licencia
Identificadores
Colecciones
Articulos(CEDIE)
Articulos de CENTRO DE INVESTIGACIONES ENDOCRINOLOGICAS "DR. CESAR BERGADA"
Articulos de CENTRO DE INVESTIGACIONES ENDOCRINOLOGICAS "DR. CESAR BERGADA"
Citación
Loreti, Rosana Nazareth; Fresno Rodríguez, Cristóbal; Barrera Cano, Juan David; Andreone, Luz; Lira Albarran, Saúl; et al.; The glycan structure in recombinant human FSH affects endocrine activity and global gene expression in human granulosa cells; Elsevier; Molecular and Cellular Endocrinology; 366; 1; 5-12-2013; 68-80
Compartir
Altmétricas