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Artículo

Modeling of second-order spectrophotometric data generated by a pH-gradient flow injection technique for the determination of doxorubicin in human plasma

Alcaraz, Mirta RaquelIcon ; Schenone, Agustina VioletaIcon ; Culzoni, Maria JuliaIcon ; Goicoechea, Hector CasimiroIcon
Fecha de publicación: 09/2013
Editorial: Elsevier Science
Revista: Microchemical Journal
ISSN: 0026-265X
Idioma: Inglés
Tipo de recurso: Artículo publicado
Clasificación temática:
Otras Ciencias Químicas

Resumen

In this paper, the development of a pH-gradient flow-injection method with diode-array spectrophotometric detection combined with extended multivariate curve resolution–alternating least-squares (MCR–ALS) to quantitate doxorubicin in the presence of rifampicin in plasma samples is presented. Quantitation of doxorubicin was based on acid–base and spectral differences between its species and those for the interference. Overlapped profiles among the analyte and the interference were mathematically solved by data modeling with the well-known extended MCR–ALS algorithm. The method, which involves a simple sample pretreatment consisting in the addition of trichloroacetic acid followed by centrifugation, was applied to resolve validation mixtures composed of human plasma spiked with doxorubicin. Acceptable analytical figures of merit were obtained, i.e. recoveries from 96 to 107%, coefficients of variation between 0.2% and 7.8%, and limits of detection and quantification of 0.77 and 2.32 μg L− 1, respectively.
Palabras clave: Multivariate Curve Resolution , Doxorubicin , Human Plasma , Second-Order Calibration
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info:eu-repo/semantics/openAccess Excepto donde se diga explícitamente, este item se publica bajo la siguiente descripción: Atribución-NoComercial-SinDerivadas 2.5 Argentina (CC BY-NC-ND 2.5 AR)
Identificadores
URI: http://hdl.handle.net/11336/31033
DOI: http://dx.doi.org/10.1016/j.microc.2013.09.012
URL: http://www.sciencedirect.com/science/article/pii/S0026265X13001677
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Articulos(CCT - SANTA FE)
Articulos de CTRO.CIENTIFICO TECNOL.CONICET - SANTA FE
Citación
Goicoechea, Hector Casimiro; Culzoni, Maria Julia; Schenone, Agustina Violeta; Alcaraz, Mirta Raquel; Modeling of second-order spectrophotometric data generated by a pH-gradient flow injection technique for the determination of doxorubicin in human plasma; Elsevier Science; Microchemical Journal; 112; 9-2013; 25-33
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