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dc.contributor.author
Morvillo, V.
dc.contributor.author
Luthy, Isabel Alicia
dc.contributor.author
Bravo, A. I.
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Capurro, M. I.
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Portela, P.
dc.contributor.author
Calandra, Ricardo Saul
dc.contributor.author
Mordoh, Jose
dc.date.available
2017-12-17T00:55:32Z
dc.date.issued
2002-12
dc.identifier.citation
Morvillo, V.; Mordoh, Jose; Calandra, Ricardo Saul; Portela, P.; Capurro, M. I.; Bravo, A. I.; et al.; Androgen receptors in human melanoma cell lines IIB-MEL-LES and IIB-MEL-IAN and in human melanoma metastases; Lippincott Williams; Melanoma Research; 12; 6; 12-2002; 529-538
dc.identifier.issn
0960-8931
dc.identifier.uri
http://hdl.handle.net/11336/30847
dc.description.abstract
The presence and characteristics of androgen receptors (AR) have been described by our group in one human melanoma cell line. We now investigated their presence in two other human melanoma cell lines, IIB-MEL-LES and IIB-MEL-IAN, as well as in biopsies of human metastatic melanoma. Scatchard analysis revealed a single binding component for both cell lines, the apparent dissociation constant obtained being 15 nM, with a binding capacity of 280 fmol/mg total cell protein for IIB-MEL-LES cells and of 14 nM with a binding capacity of 206 fmol/mg total cell protein for IIB-MEL-IAN. When specificity was assessed, as seen before, not only androgen and antiandrogen, but also non-androgenic compounds were able to compete for [3H]-R1881 binding. When immunocytochemistry of IIB-MEL-LES and IIB-MEL-IAN cells was performed for AR, both cell lines were deeply stained in the nucleus whereas no staining was found for oestrogen and progesterone receptors. Every specimen of melanoma metastases tested for the presence of AR was deeply stained, and the intensity of the staining was high in the majority. Several hormones and antihormones were tested for their ability to affect cell proliferation. On both cell lines, Testosterone, Dihydrotesterone, Oestradiol and Progesterone significantly stimulated cell proliferation, with reversion by Hydroxy-Flutamide, Casodex or Tamoxifen.
dc.format
application/pdf
dc.language.iso
eng
dc.publisher
Lippincott Williams
dc.rights
info:eu-repo/semantics/openAccess
dc.rights.uri
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.subject
Androgen
dc.subject
Androgen Receptor
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Anti-Androgen
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Anti-Estrogen
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Flutamide
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Melanoma
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Tamoxifen
dc.subject.classification
Patología
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Medicina Básica
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CIENCIAS MÉDICAS Y DE LA SALUD
dc.title
Androgen receptors in human melanoma cell lines IIB-MEL-LES and IIB-MEL-IAN and in human melanoma metastases
dc.type
info:eu-repo/semantics/article
dc.type
info:ar-repo/semantics/artículo
dc.type
info:eu-repo/semantics/publishedVersion
dc.date.updated
2017-12-04T17:54:09Z
dc.identifier.eissn
1473-5636
dc.journal.volume
12
dc.journal.number
6
dc.journal.pagination
529-538
dc.journal.pais
Reino Unido
dc.journal.ciudad
Londres
dc.description.fil
Fil: Morvillo, V.. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
dc.description.fil
Fil: Luthy, Isabel Alicia. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biología y Medicina Experimental. Fundación de Instituto de Biología y Medicina Experimental. Instituto de Biología y Medicina Experimental; Argentina
dc.description.fil
Fil: Bravo, A. I.. Provincia de Buenos Aires. Ministerio de Salud. Hospital Interzonal de Agudos ; Argentina
dc.description.fil
Fil: Capurro, M. I.. Instituto Luis Federico Leloir;
dc.description.fil
Fil: Portela, P.. Instituto Luis Federico Leloir;
dc.description.fil
Fil: Calandra, Ricardo Saul. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biología y Medicina Experimental. Fundación de Instituto de Biología y Medicina Experimental. Instituto de Biología y Medicina Experimental; Argentina. Universidad Nacional de La Plata. Facultad de Ciencias Exactas; Argentina
dc.description.fil
Fil: Mordoh, Jose. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Investigaciones Bioquímicas de Buenos Aires. Fundación Instituto Leloir. Instituto de Investigaciones Bioquímicas de Buenos Aires; Argentina
dc.journal.title
Melanoma Research
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/url/https://insights.ovid.com/pubmed?pmid=12459642
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/doi/http://dx.doi.org/10.1097/01.cmr.0000043153.28051.51
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/pmid/12459642
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