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dc.contributor.author
Morvillo, V.
dc.contributor.author
Luthy, Isabel Alicia
dc.contributor.author
Bravo, A. I.
dc.contributor.author
Capurro, M. I.
dc.contributor.author
Portela, P.
dc.contributor.author
Calandra, Ricardo Saul
dc.contributor.author
Mordoh, Jose
dc.date.available
2017-12-17T00:55:32Z
dc.date.issued
2002-12
dc.identifier.citation
Morvillo, V.; Mordoh, Jose; Calandra, Ricardo Saul; Portela, P.; Capurro, M. I.; Bravo, A. I.; et al.; Androgen receptors in human melanoma cell lines IIB-MEL-LES and IIB-MEL-IAN and in human melanoma metastases; Lippincott Williams; Melanoma Research; 12; 6; 12-2002; 529-538
dc.identifier.issn
0960-8931
dc.identifier.uri
http://hdl.handle.net/11336/30847
dc.description.abstract
The presence and characteristics of androgen receptors (AR) have been des­cribed by our group in one human melanoma cell line. We now investigated their presence in two other human melanoma cell lines, IIB-MEL-LES and IIB-MEL-IAN, as well as in biopsies of human metastatic melanoma. Scatchard analysis revealed a single binding component for both cell lines, the apparent dissociation constant obtained being 15 nM, with a binding capacity of 280 fmol/mg total cell protein for IIB-MEL-LES cells and of 14 nM with a binding capacity of 206 fmol/mg total cell protein for IIB-MEL-IAN. When specifi­ci­ty was assessed, as seen before, not only androgen and anti­an­dro­gen, but also non-andro­ge­nic com­pounds were able to compete for [3H]-R1881 binding. When immu­nocyto­che­mis­try of IIB-MEL-LES and IIB-MEL-IAN cells was performed for AR, both cell lines were deeply stained in the nu­cleus whe­reas no staining was found for oestrogen and pro­ges­te­rone receptors. Every specimen of mela­no­ma metas­ta­ses tested for the presence of AR was deeply stained, and the intensity of the stai­ning was high in the majority. Several hormones and antihormones were tested for their ability to affect cell proli­fe­ration. On both cell lines, Testosterone, Dihydrotesterone, Oestradiol and Pro­ges­terone significantly stimulated cell proliferation, with reversion by Hydroxy-Flutamide, Casodex or Tamo­xi­fen.
dc.format
application/pdf
dc.language.iso
eng
dc.publisher
Lippincott Williams
dc.rights
info:eu-repo/semantics/restrictedAccess
dc.rights.uri
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.subject
Androgen
dc.subject
Androgen Receptor
dc.subject
Anti-Androgen
dc.subject
Anti-Estrogen
dc.subject
Flutamide
dc.subject
Melanoma
dc.subject
Tamoxifen
dc.subject.classification
Patología
dc.subject.classification
Medicina Básica
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CIENCIAS MÉDICAS Y DE LA SALUD
dc.title
Androgen receptors in human melanoma cell lines IIB-MEL-LES and IIB-MEL-IAN and in human melanoma metastases
dc.type
info:eu-repo/semantics/article
dc.type
info:ar-repo/semantics/artículo
dc.type
info:eu-repo/semantics/publishedVersion
dc.date.updated
2017-12-04T17:54:09Z
dc.identifier.eissn
1473-5636
dc.journal.volume
12
dc.journal.number
6
dc.journal.pagination
529-538
dc.journal.pais
Reino Unido
dc.journal.ciudad
Londres
dc.description.fil
Fil: Morvillo, V.. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
dc.description.fil
Fil: Luthy, Isabel Alicia. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biología y Medicina Experimental. Fundación de Instituto de Biología y Medicina Experimental. Instituto de Biología y Medicina Experimental; Argentina
dc.description.fil
Fil: Bravo, A. I.. Provincia de Buenos Aires. Ministerio de Salud. Hospital Interzonal de Agudos ; Argentina
dc.description.fil
Fil: Capurro, M. I.. Instituto Luis Federico Leloir;
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Fil: Portela, P.. Instituto Luis Federico Leloir;
dc.description.fil
Fil: Calandra, Ricardo Saul. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biología y Medicina Experimental. Fundación de Instituto de Biología y Medicina Experimental. Instituto de Biología y Medicina Experimental; Argentina. Universidad Nacional de La Plata. Facultad de Ciencias Exactas; Argentina
dc.description.fil
Fil: Mordoh, Jose. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Investigaciones Bioquímicas de Buenos Aires. Fundación Instituto Leloir. Instituto de Investigaciones Bioquímicas de Buenos Aires; Argentina
dc.journal.title
Melanoma Research
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/url/https://insights.ovid.com/pubmed?pmid=12459642
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/doi/http://dx.doi.org/10.1097/01.cmr.0000043153.28051.51
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/pmid/12459642


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